Microplate Capacity
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence modes: plates up to 1.25” (31.75 mm) high
Environment
Operational temperature 18º to 40°C
Humidity
10% to 85% relative humidity (non-condensing)
Power Supply
24-volt external power supply compatible with
100-240 V~; +10% @50-60 Hz
Power Consumption
250W maximum
Incubation
Temperature control range from 4º over ambient to 50ºC.
Temperature variation ± 0.50°C across the plate @ 37°C (250 µl per well with the plate sealed)
Plate Shaking
Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user
Luminescence
50 amol/well flash ATP in a 96-well plate (low-noise #49984 PMT)
500 amol/well flash ATP in a 96-well plate (red-shifted #49721 PMT)
Sample Size
1 microliter
Path Length
1mm (with auto-ranging to 0.2 mm)
Light Source
Xenon flash lamp
Detector Type
2048-element linear silicon CCD array
Wavelength Range
220 to 750 nm
Wavelength Accuracy
1 nm
Wavelength Resolution
3 nm (FWHM at Hg 546 nm)
Absorbance Precision
0.003 absorbance (1mm path)
Absorbance Range
0.02 to 75 (10 mm equivalent absorbance)
Detection Limit
2 ng/microliter (dsDNA)
Maximum Concentration
3700 ng/microliter (dsDNA)
Measurement Cycle Time
10 seconds
Dimensions
14 x 20 cm
Wavelength Range:
340—850 nm
Wavelength Selection
Monochromator, tunable in 1-nm increments
Wavelength Bandwidth
≤ 2.0 nm full width half maximum
± 1.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm across all optical channels
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy/Linearity
< ± 1.0% and ± 0.006 OD, 0.0-2.0 OD
Photometric Precision
< ± 1.0% and ± 0.003 OD, 0.0-2.0 OD
Stray Light
< 0.05% @ 340 nm
Xenon Flash Lamp
Microplate Read Time – Endpoint
9 seconds
Microplate Read Time – Kinetic
9-second minimum interval
Temperature Range
Ambient + 4° to 45°C
Temperature Uniformity
±0.5 °C @ 37 °C well-to-well
Performance Specifications
190 - 850 nm
Monochromator, tunable 1.0 nm increments
2 nm
Wavelength Accuracy:
< ± 2.0 nm
± 0.2 nm
0.0 to 4.0 OD
Photometric Accuracy
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
< 0.05% @ 230 nm
Light Source:
Endpoint:
12 seconds
Kinetic:
9 second minimum interval
Ambient + 4 °C up to 45 °C
Temperature Uniformity:
± 0.5 °C @ 37 °C
Standard 96- and 384-well microplates
Dimensions (D x W x H)
16 x 8.5 x 8.5 in (40.6 x 21.6 x 21.6 cm)
Weight
24 lb. (10.9 kg)
Absorbance: Xenon flash light source
100 VA max
Temperature control range from 4º over ambient to 50ºC
Temperature variation ± 0.50°C across the plate @ 37°C (250 µL per well with the plate sealed)
Low, Medium, High and Variable shaking speeds. Duration is programmable by the user
Read Modes
Normal, Rapid and Sweep
Absorbance Measurement Range
Absorbance Accuracy
2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates0.000 to 1.000 OD ± 1% ± 0.010 OD Sweep mode, 96- and 384-well plates
Temperature control range from Ambient +4°C to 50°C
3% (at 0.74 Abs at 350nm)
Pedestal: 0-300 ABS Cuvette: 0 - 1.5 ABS
Accuracy
0.002
Accuracy (Absorption)
3% at (0.74 at 350nm)
Compatibility
Microsoft™ Windows™ XP (32-bit) w/Service Pack (SP) 2 or later, Vista™ (32-bit), Windows 7 (32 bit and 64 bit), Windows 10 (64 bit)
Concentration
2ng/µl-15,000ng/µl (dsDNA) ng/µl
Detection Limits
Pedestal: 2ng/µL (dsDNA), 0.10mg/mL (BSA) Cuvette: 0.4ng/µL (dsDNA), 0.01 mg/mL (BSA)
Detection Range
Pedestal: 2-15,000ng/µL (dsDNA) , 0.10 - 400mg/mL (BSA)
14 x 20cm
Heating Range
37°; ±0.5°C
Lamp
Xenon Flash
Measurement Time
<3 sec.
No. of Samples
1
Pathlength (Metric)
10; 5; 2; 1mm
12VDC, 5W
Sample Volume (Metric)
1000, 0.5-2.0µL
Spectral Resolution
=1.8nm (FWHM at Hg 253.7)
System Requirements
Microsoft Windows 7 Professional (32-bit and 64-bit), Windows 8 (32- and 64-bit), and Windows 10 Pro (64-bit)
±1nm
190-840nm
Spectral Range
190-1000 nm, tunable in 1 nm increments
190-1000 nm
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
< ± 1.0 nm
Photometric Accuracy (plate)
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
9 nm
Wavelength
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Ambient + 4° up to 45°C
± 1° at 37°C
Fluorescence Photometric Performance
Dual Monochromators
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
Time Resolved Fluorescence
Data Collection
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
360-850nm
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Detector
Photomultiplier (R-3896)
Read Time
96 < 15 sec, 384 < 45 sec
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C - 45°C
Luminescence/Fluorescence modes: plates up to 1.25” (31.75 mm) high Absorbance mode: plates up to 0.8” (20.30 mm) high
Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
100 VA max, 130 VA max with injectors
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
≤ 4.0 nm
± 2.0 nm
0 to 4.0 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
≤ 4.0 nm full width half maximum
± 2.0 nm across wavelength range
Fluorescence Intensity Performance
≤ 1 pM (fluorescein) in 96 wells≤ 1.5 pM (fluorescein) in 384 wells
< 5 pM (fluorescein) in 96 wells or cuvette< 20 pM in 384 wells
Luminescence Photometric Performance
< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
Crosstalk
< 0.5% in 96- and 384- well microplates
Optimized Sensitivity
≤ 43 pM ATP in 96 wells
≤ 75 pM ATP in 96 wells
Detectors
2 Photomultiplier tubes (PMT)
96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
Ambient +2°C to 60°C
Dimensions (H x W x D)
21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
16.4 kg (36 lbs)
UV/Vis Photometric Performance:
200 – 1000 nm
Monochromator, tunable in 1.0 nm increments
0 – 4.0 OD
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments
EX 250 – 850 nm
EM 360 – 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells
UV/Vis Photometric Performance
200 - 1000 nm
0 - 4.0 OD
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
General Specifications:
96 well (Abs 18 sec, FI 15 sec) 384 well (Abs 49 sec, FI 45 sec)
Ambient +4 °C to 45°C
± 1 °C @ 37°C
± 0.5°C @ 37°C set point
Power
110 - 120 Vac 50/60 Hz
