Wavelength Range
340 to 850 nm
Wavelength Selection
Monochromator, tunable in 1-nm increments
Wavelength Bandwidth
± 2.0 nm full width half maximum
Wavelength Accuracy
± 1.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm across all optical channels
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy/Linearity
< ± 1.0% and ± 0.006 OD, 0.0-2.0 OD
Photometric Precision
< ± 1.0% and ± 0.003 OD, 0.0-2.0 OD
Stray Light
< 0.05% @ 340 nm
Light Source
Xenon Flash Lamp
Microplate Read Time ‚ Endpoint
9 seconds
Microplate Read Time ‚ Kinetic
9-second minimum interval
Temperature Range
Ambient + 4 to 45C
Temperature Uniformity
±0.5C @ 37C well-to-well
Fluorescence: 250 to 850 nm (Excitation & Emission)
Luminescence: 360 to 850 nm
Absorbance Specifications
Xenon flash lamp
Double monochromator
230 to 999 nm, in 1 nm increments
Bandpass
2 nm (230-285 nm), 4 nm (>285 nm)
Pathlength Correction
Yes
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
± 0.2 nm
Dynamic Range
0 to 4.0 OD
0.0001 OD
Photometric Accuracy
< 1% at 2.0 OD typical
Photometric Linearity
< 1% from 0 to 3.0 OD
Photometric Repeatability
< 0.5% at 2.0 OD
Reading Speed
96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity Specifications
Detector
Dedicated red shifted PMT
Double grating monochromators (top read only)
250 to 900 nm
5 decades
Sensitivity (Top Read Only)
Fluorescein 2 pM typical (0.2 fmol/well, 384-well plate)
Luminescence Specifications
< 6 decades
Sensitivity
10 amol ATP typical (flash)
Detection System
Low noise PMT
300 to 700 nm
Absorbance: 200 to 999 nm
Fluorescence: 300 to 650 nm (Excitation) / 300 to 700 nm (Emission)
Luminescence: 300 to 700 nm
Absorbance: Monochromator, ± 1 nm
Fluorescence: Filter Based
200 to 999 nm
Wavelength Bandpass
2.4 nm
Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates
Incubation
Temperature control range from Ambient +4º to 50ºC
Temperature variation ± 0.5°C at 37°C
Plate Shaking
Linear, Orbital Slow, and Orbital Fast
Read Modes
Normal and Sweep
Absorbance Measurement Range
Absorbance Accuracy
96-Well Plate, Normal Read Speed:0.000 to 2.000 OD ± 1.0% ± 0.010 OD2.000 to 3.000 OD ± 3.0% ± 0.010 OD384-Well Plate, Normal Read Speed:0.000 to 2.000 OD ± 2.0% ± 0.010 OD2.000 to 2.500 OD ± 3.0% ± 0.010 OD96-Well/384-Well Plate, Sweep Read Speed:0.000 to 1.000 OD ± 1% ± 0.010 OD
Absorbance: 200 to 1000 nm
Fluorescence: 250 to 850 nm (Excitation) / 360 to 850 nm (Emission)
Luminescence: 250 to 850 nm
Absorbance: ≤ 4.0 nm
Fluorescence: 9.0 nm
Fluorescence: Dual Monochromators, ± 1 nm
Fluorescence: 300 to 700 nm (Excitation & Emission)
Fluorescence: 9.0 nm (Excitation) / 15 nm (Emission)
Fluorescence: 250 to 850 nm (Excitation) / 250 to 850 nm (Emission)
