Wavelength Range
340 to 900 nm
340, 405, 450, 490, and 630 nm
Wavelength Bandpass
10 nm
Standard 96-well microplates
Incubation
Temperature control up to 50ºC
Temperature uniformity ± 0.5°C at 37°C
Plate Shaking
Low, Medium, High, and Variable Speeds
Read Modes
Endpoint, Kinetic, and Linear Scan
Absorbance Measurement Range
0.000 to 4.000 OD (400 to 900 nm range) 0.000 to 3.000 OD (340 to 400 nm range)
Absorbance Accuracy
± 1.0% ± 0.010 OD from 0.000 to 2.500 OD @ 405 nm
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 340 nm
400 to 750 nm (Filter-Based)
Filters Included
405, 450, 490, and 630 nm
Microplate Capacity
Standard 6-, 12-, 24-, 48-, and 96-well microplates
N/A
Endpoint, Kinetic, and Well Area Scanning
0.000 to 3.000 OD
96-Well Plate, Normal Read Mode:
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD at 405 nm
96-Well Plate, Rapid Read Mode:
± 2.0% ± 0.020 OD from 0.000 to 2.000 OD at 405 nm
0.000 to 4.000 OD
2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates0.000 to 1.000 OD ± 1% ± 0.010 OD Sweep mode, 96- and 384-well plates
Temperature control range from Ambient +4°C to 50°C
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth
9 nm
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Light Source
Xenon flash lamp
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
Fluorescence Photometric Performance
Dual Monochromators
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
Time Resolved Fluorescence
Data Collection
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
360-850nm
Detection Limit
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Detector
Photomultiplier (R-3896)
Read Time
96 < 15 sec, 384 < 45 sec
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C - 45°C
Spectral Range
190-1000 nm, tunable in 1 nm increments
190-1000 nm
Wavelength Selection
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
2 nm
Wavelength Accuracy
< ± 1.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0.0 to 4.0 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (plate)
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Xenon Flash Lamp
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates Max Plate Height:
Luminescence/Fluorescence modes: plates up to 1.25" (31.75 mm) high
Absorbance mode: plates up to 0.8" (20.30 mm) high
Absorbance Specifications
Photodiode
Monochromator
200 to 999 nm, in 1 nm increments
Monochromator Bandwidth
2.4 nm
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
0 to 4.0 OD
0.0001 OD
Pathlength Correction
Yes
Photometric Linearity
< 1% from 0 to 3.0 OD
Photometric Repeatability
< 0.5% at 2.0 OD
Fluorescence Intensity Specifications
Tungsten halogen
PMT
Filter-based
300 to 700 nm
> 6 decades
Sensitivity (Top and Bottom)
Fluorescein 5 pM (1 fmol/well, 96-well plate)
Luminescence Specifications
< 6 decades
30 amol ATP (flash)
Detection System
Low noise PMT
Alpha Detection Specifications
300 amol of biotinylated LCK-P peptide
Read Speed
2 minutes (96-well plate)
6, 12, 24, 48, 96, 384 wells
Read Methods
End point, kinetic, spectral scanning, well-area scanning
Linear and orbital
4-Zone incubation to 50°C, ± 0.2°C at 37°C
Dimensions (H x W x D)
12 x 15 x 15 in. (30.5 x 38.1 x 38.1 cm)
Weight
27 lb. (12.3 kg.)
100-240V, 50/60Hz
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
± 4.0 nm
± 2.0 nm
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Ambient +4C to 45C
< 1C at 37C set point
Temperature Accuracy
±1C at 37C set point
≤ 4.0 nm
9 nm, 9 nm
± 1C at 37C set point
≤ 4.0 nm full width half maximum
± 2.0 nm across wavelength range
Fluorescence Intensity Performance
≤ 1 pM (fluorescein) in 96 wells≤ 1.5 pM (fluorescein) in 384 wells
< 5 pM (fluorescein) in 96 wells or cuvette< 20 pM in 384 wells
Luminescence Photometric Performance
< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
Crosstalk
< 0.5% in 96- and 384- well microplates
Optimized Sensitivity
≤ 43 pM ATP in 96 wells
≤ 75 pM ATP in 96 wells
Detectors
2 Photomultiplier tubes (PMT)
96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
Ambient +2°C to 60°C
< 1°C at 37°C set point
±1°C at 37°C set point
21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
16.4 kg (36 lbs)
UV/Vis Photometric Performance:
Monochromator, tunable in 1.0 nm increments
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments
EX 250 to 850 nm
EM 360 to 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells