Microplate Capacity
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence modes: plates up to 1.25” (31.75 mm) high
Environment
Operational temperature 18º to 40°C
Humidity
10% to 85% relative humidity (non-condensing)
Power Supply
24-volt external power supply compatible with
100-240 V~; +10% @50-60 Hz
Power Consumption
250W maximum
Incubation
Temperature control range from 4º over ambient to 50ºC.
Temperature variation ± 0.50°C across the plate @ 37°C (250 µl per well with the plate sealed)
Plate Shaking
Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user
Luminescence
50 amol/well flash ATP in a 96-well plate (low-noise #49984 PMT)
500 amol/well flash ATP in a 96-well plate (red-shifted #49721 PMT)
Wavelength Range
340—850 nm
Wavelength Selection
Monochromator, tunable in 1-nm increments
Wavelength Bandwidth
≤ 2.0 nm full width half maximum
Wavelength Accuracy
± 1.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm across all optical channels
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy/Linearity
< ± 1.0% and ± 0.006 OD, 0.0-2.0 OD
Photometric Precision
< ± 1.0% and ± 0.003 OD, 0.0-2.0 OD
Stray Light
< 0.05% @ 340 nm
Light Source
Xenon Flash Lamp
Microplate Read Time – Endpoint
9 seconds
Microplate Read Time – Kinetic
9-second minimum interval
Temperature Range
Ambient + 4° to 45°C
Temperature Uniformity
±0.5 °C @ 37 °C well-to-well
Wavelength Range:
Performance Specifications
190 - 850 nm
Monochromator, tunable 1.0 nm increments
2 nm
Wavelength Accuracy:
< ± 2.0 nm
± 0.2 nm
0.0 to 4.0 OD
Photometric Accuracy
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
< 0.05% @ 230 nm
Light Source:
Xenon flash lamp
Endpoint:
12 seconds
Kinetic:
9 second minimum interval
Ambient + 4 °C up to 45 °C
Temperature Uniformity:
± 0.5 °C @ 37 °C
Spectral Range
190-1000 nm, tunable in 1 nm increments
190-1000 nm
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
< ± 1.0 nm
Photometric Accuracy (plate)
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
9 nm
Wavelength
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Ambient + 4° up to 45°C
± 1° at 37°C
Fluorescence Photometric Performance
Dual Monochromators
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
Time Resolved Fluorescence
Data Collection
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
360-850nm
Detection Limit
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Detector
Photomultiplier (R-3896)
Read Time
96 < 15 sec, 384 < 45 sec
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C - 45°C
Luminescence/Fluorescence modes: plates up to 1.25” (31.75 mm) high Absorbance mode: plates up to 0.8” (20.30 mm) high
Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
100 VA max, 130 VA max with injectors
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
≤ 4.0 nm
± 2.0 nm
0 to 4.0 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
UV/Vis Photometric Performance:
200 – 1000 nm
Monochromator, tunable in 1.0 nm increments
0 – 4.0 OD
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments
EX 250 – 850 nm
EM 360 – 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells
UV/Vis Photometric Performance
200 - 1000 nm
0 - 4.0 OD
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
General Specifications:
96 well (Abs 18 sec, FI 15 sec) 384 well (Abs 49 sec, FI 45 sec)
Ambient +4 °C to 45°C
± 1 °C @ 37°C
± 0.5°C @ 37°C set point
Power
110 - 120 Vac 50/60 Hz
