Setable Flow Range

Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments

Flow Range

0.1 to 5 ml/min

Flow Precision

≤ 0.07% RSD or ≤ 0.02 min SD

Flow Accuracy

± 1% or 10 ul/min

Pressure

Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min

Pressure Pulsation

< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility Compensation

User-selectable, based on mobile phase compressibility

Hydraulic System

Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve

Recommended pH Range

1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel

Gradient Formation

High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure

Composition Range

1 to 99% or 5 ul/min per channel

Composition Precision

≤ 0.5% absolute

Composition Accuracy

≤ 0.15% RSD at 1 ml/min

Temperature Range

5°C to 550°C

Stirrer Speed

60 to 1,150 RPM

Platform Size

10 x 10 in (25.4 x 25.4 cm)

Electrical Requirements

120V, 60Hz, 9.3A, 1113W

Max Flow Rate

10 ml/min per channel

Reduced performance above 5 ml/min

Number of Channels

4

Internal Volume

1 ml/channel

Materials in Contact with Solvent

PTE, PEEK

pH Range

1 – 14

Analog Output (AUX)

For pressure monitoring, range 0 – 3 V

Dimensions (W x D x H)

13.5 x 17 x 3.1 in (345 x 435 x 80 mm)

Electrical Requirements

100-120 or 220-240 VAC, 50/60 Hz

Vacuum Reading Range

0 to 1400 Millibar

Hysteresis

Automatic or 1 – 500 mbar

Regulating range

1 mbar/torr to atmospheric pressure

Display

Digital LCD Display

Power Consumption

16 watts

Electrical Requirements

100-240 VAC, 50/60 Hz

Performance

Wavelength Range Excitation

250 to 850 nm

Wavelength Range Emission

250 to 850 nm

Wavelength Selections

Monochromator, tunable 1.0 nm increments

Wavelength Bandwidth

9 nm

Wavelength

< ± 2.0 nm

Top Read Sensitivity

3.0 fmol/well FITC 200 µl in 96 wells

Bottom Read Sensitivity

8.0 fmol/well FITC 200 µl in 96 wells

Light Source

Xenon flash lamp

Microplate Read Time

Normal Mode

96 wells in 15 seconds

384 wells in 45 seconds

Dynamic Range

6 orders of magnitude

Temperature Range

Ambient + 4° up to 45°C

Temperature Uniformity

± 1° at 37°C

Fluorescence Detection Wavelength

Bandpass 547 to 750 nm

Fluorescence Data Rates

80Hz

Light Source Warmup Time

30 seconds

Light Source Excitation/Emission Wavelengths

532 nm

Light Source Power Output

5 mW maximum continuous (CW)

Dimensions (H x W x D)

7.1 x 7.1 x 13 in (180 x 180 x 330 mm)

Chip Capacity

One 4-channel chip

Ventilation/Cooling

3” (77 mm) minimum space required around instrument for proper air flow

Operating Temperature

65° to 82°F (18° to 28°C)

Operating Humidity

10% to 70% RIH, noncondensing

Input Voltage

100-127/200-240V~ nominal

90-264V~ maximum operating range