Agilent BioTek Synergy HTX Multi-Mode Microplate Reader

The Agilent BioTek Synergy HTX Multi-Mode Microplate Reader is a versatile and high-performing instrument designed for a variety of laboratory applications. It features UV-Vis absorbance, filter-based fluorescence, and luminescence capabilities, making it suitable for a variety of different applications. The reader’s unique dual-optics design includes a xenon flash lamp and monochromator for absorbance measurements across a wide wavelength range (200 to 999 nm) and a tungsten halogen lamp with interference filters for sensitive fluorescence detection. This compact system is further enhanced with the Agilent BioTek's unique 4-Zone incubation up to 50°C, the ability to add-on dual reagent injectors, and options for linear and orbital shaking, accommodating 6- to 384-well microplates. The Synergy HTX is compatible with the user-friendly Agilent BioTek Gen5 software, streamlining data collection, analysis, exporting, and reporting.

Additionally, the Synergy HTX is designed for integration with the BioTek BioStack microplate stacker for increased automation and throughput, capable of processing up to 50 microplates simultaneously. The reader is ideal for a range of applications, including nucleic acid and protein quantification, enzyme kinetics, biomarker quantification, ELISAs, genetic analysis, cell proliferation, cytotoxicity, drug absorption and metabolism, food safety, and environmental monitoring. It's also equipped for low volume (2 μL) nucleic acid quantification with Take3 and Take3 Trio plates, and features an advanced incubator design with Condensation Control to minimize plate lid condensation. The device is alpha assay capable, modular, upgradable, and compatible with third-party automation solutions, offering convenience, versatility, and affordability for various scientific research needs.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Agilent BioTek Synergy HTX Multi-Mode Microplate Reader Specifications

Absorbance Specifications
Light Source	Xenon flash lamp
Detector	Photodiode
Wavelength Selection	Monochromator
Wavelength Range	200 to 999 nm, in 1 nm increments
Monochromator Bandwidth	2.4 nm
Monochromator Wavelength Accuracy	± 2 nm
Monochromator Wavelength Repeatability	± 0.2 nm
Dynamic Range	0 to 4.0 OD
Photometric Resolution	0.0001 OD
Pathlength Correction	Yes
Photometric Linearity	< 1% from 0 to 3.0 OD
Photometric Repeatability	< 0.5% at 2.0 OD
Fluorescence Intensity Specifications
Light Source	Tungsten halogen
Detector	PMT
Wavelength Selection	Filter-based
Wavelength Range	300 to 700 nm
Dynamic Range	> 6 decades
Sensitivity (Top and Bottom)	Fluorescein 5 pM (1 fmol/well, 96-well plate)
Luminescence Specifications
Dynamic Range	< 6 decades
Sensitivity	30 amol ATP (flash)
Detection System	Low noise PMT
Wavelength Range	300 to 700 nm
Alpha Detection Specifications
Light Source	Tungsten halogen
Sensitivity	300 amol of biotinylated LCK-P peptide
Read Speed	2 minutes (96-well plate)
General Specifications
Microplate Formats	6, 12, 24, 48, 96, 384 wells
Read Methods	End point, kinetic, spectral scanning, well-area scanning
Plate Shaking	Linear and orbital
Temperature Control	4-Zone incubation to 50°C, ± 0.2°C at 37°C
Dimensions (H x W x D)	12 x 15 x 15 in. (30.5 x 38.1 x 38.1 cm)
Weight	27 lb. (12.3 kg.)
Electrical Requirements	100-240V, 50/60Hz

Computer Specifications
Model	Hewlett-Packard
Processor	Intel Core i5-7200U CPU
RAM	16.0 GB
Hard Drive	240GB Internal SSD
Operating System	Windows 10 Professional, 64-bit