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Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader
The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection
microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have optical performance comparable to a
top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint,
kinetic, spectrum and area-well scanning read types and PathCheck allow
homogeneous and heterogeneous microplate assays to be performed in this
flexible system. This FI, Abs, and Lumi microplate reader with a built-in cuvette port
allows for easy conversion and optimization of very-low-throughput assays into
medium-high-throughput microplate assays.
The SpectraMax M3 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The SpectraMax M3 features top-read and bottom-read modes, resulting in greater sensitivity for solution and cell-based assays. Within endpoint and kinetic measurement protocols, up to four wavelength pairs can be read at a time, allowing for improved efficiency for FRET assays. For Luminescence, the SpectraMax M3 utilizes a dedicated luminescence photomultiplier tube (PMT), providing the maximum signal and lowest background noise as possible for glow luminescence report gene assays. Key Features: dual mode cuvette port, robotics compatible, multilabel, dual monochromators, and PathCheck.
Applications include: DNA, RNA, and protein quantitation and purity; PicoGreen, NanoOrange, and Bradford; ELISAs and enzyme kinetics; drug dissolution profiles; live or dead viability or cytotoxicity assays; Caspase-3 and protease assays; cAMP assays using CatchPoint Assay Kits; and low volume applications.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader includes:
- > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 6.5
- > Interface Cable
- > Molecular Devices Absorbance and Fluorescence Validation Report
- > Top Read Plate
- > Power Cord
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Specifications
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Absorbance Photometric Performance
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Wavelength Selection
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Monochromator, tunable in 1 nm increments
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Wavelength Range
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200 to 1000 nm
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Wavelength Bandwidth
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≤ 4.0 nm full width half maximum
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Wavelength Accuracy
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± 2.0 nm across wavelength range
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0.000 to 4.000 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (Microplate)
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< ± 0.006 OD ± 1%, 0 to 2 OD
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Photometric Accuracy (Cuvette)
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< ± 0.005 OD ± 1%, 0 to 2 OD
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Photometric Precision
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< ± 0.003 OD ± 1%, 0 to 2 OD
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Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Fluorescence Intensity Performance
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Dual Monochromators
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1 nm increment selection
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EX Wavelength Range
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250 to 850 nm
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EM Wavelength Range
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250 to 850 nm
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Wavelength Bandwidth (EX, EM)
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9 nm
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Top-Read Optimized Sensitivity
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≤ 1 pM (fluorescein) in 96 wells
≤ 1.5 pM (fluorescein) in 384 wells
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Top-Read Guaranteed Sensitivity
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< 5 pM (fluorescein) in 96 wells or cuvette
< 20 pM in 384 wells
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Luminescence Photometric Performance
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Sensitivity
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< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
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Wavelength Range
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250 to 850 nm
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Crosstalk
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< 0.5% in 96- and 384- well microplates
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Dynamic Range
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Optimized Sensitivity
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≤ 43 pM ATP in 96 wells
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Guaranteed Sensitivity
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≤ 75 pM ATP in 96 wells
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General Photometric Performance
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Plate Formats
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6, 12, 24, 48, 96, 384 wells
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Light Source
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Xenon flash lamp (1 joule/flash)
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Detectors
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2 Photomultiplier tubes (PMT)
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Read Time
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96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
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Shaker Time
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0 to 999 seconds
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Temperature Control
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Ambient +2°C to 60°C
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Temperature Uniformity
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< 1°C at 37°C set point
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Temperature Accuracy
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± 1°C at 37°C set point
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General Specifications
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Dimensions (H x W x D)
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21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
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Weight
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16.4 kg (36 lbs)
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Computer Specifications
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Model
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Hewlett-Packard
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Processor
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Intel Core i5-4300U CPU
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RAM
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8GB
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Hard Drive
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240GB Internal SSD
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Operating System
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Windows 10 Professional, 64-bit
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- Confirm PCB And Power Supply Are Outputting Proper Voltages
- Inspect Display and Replace Any Dim/Dead Pixels
- Inspect Buttons and Confirm Full Functionality
- Check Functionality and Clearance of Plate Drawer
- Confirm Communication Between Plate Reader and Computer Software
- Inspect Internal Wiring and Electrical Connection
- Inspect Lamp and Replace as Needed
- Perform Appropriate Validation Protocol
- Perform All Current Updates for Data Station And Operating System
- Ensure Cosmetics Are Acceptable
To View More of Our Recertification Protocol Click Here
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Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.
All Lab Equipment is Fully Recertified and Tested
Most Pieces Ship within 1 to 3 Business Days
All Products are Fully Warrantied
Trained Customer and Technical Support
Industry Leading Overall Customer Satisfaction
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My recently purchased microplate reader from Marshall Scientific has been a much needed addition to my lab. The reader is easy to use and provides accurate and reliable results. I have used it for various experiments including ELISA, cell viability assays, and protein quantification. The reader has greatly improved the efficiency and accuracy of my experiments. Thanks again!
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