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Molecular Devices SpectraMax 384 Plus Microplate Reader
The Molecular Devices SpectraMax 384 Plus is a UV/Vis
spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate
drawer. The instrument reads any standard cuvette, microcuvette, test tube or
96- and 384-well standard or UV transparent microplate over a full 190 to 1000 nm
wavelength range. This 384 Plus comes from a long history of reliable tools
built by Molecular Devices. This robust system is capable of reading 96 or 384
wells, each with its own sample beam and reference detector, for optical density measurement at a selected wavelength with endpoint (for a single point in time), kinetic (over a specific period of time), or spectral scan mode (over a specific wavelength range).
The high-precision nine channel system (8 for the microplate; 1 for the cuvette port) consists of
sample beams and reference beams that mimic a dual beam spectrophotometer. The filter-less system includes monochromator, allowing for up to 6 wavelengths within the visible spectrum at a time, saving time without the need to change out filters.
The PathCheck sensor eliminates optical pathlength as a factor (and corrects
pipetting variation), closing the gap between microplate readers and
spectrophotometers. This is accomplished by PathCheck, in conjunction with the SoftMax Pro software, automatically normalizing well absorbance, allowing for the elimination of standard curves, and correcting volume differences in the wells. The 384 Plus measures liquid depth and volumes down to 5µl in
each microplate well and scales the microplate data to standard 1 cm cuvette
values. The monochromator selects wavelengths in 1nm increments, and the system is equipped
with a built-in programmable linear and orbital plate shaking device.
Key applications include: DNA and RNA analysis; ELISAs and immunoassays; cell viability, proliferation, and cytotoxicity; endpoint analysis; enzyme kinetic assays; protein quantitation; endotoxin detection; and PathCheck.
The Molecular Devices SpectraMax 384 Plus Microplate Reader includes:
- > Windows 7 Data Station with Pre-Loaded SoftMax Pro Software 6.3.1
- > Interface Cable
- > Molecular Devices Absorbance Validation Report
- > Operator's Guide
- > Power Cord
- > Full 90 Day Parts and Labor Warranty
Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications
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Spectral Range
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190-1000 nm, tunable in 1 nm increments
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Wavelength Range
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190-1000 nm
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Wavelength Selection
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Monochromator, tunable 1.0nm increments
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Speed Read Mode
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Reads 96-well microplates in 5 seconds; Reads 384-well
microplates in 16 seconds
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Wavelength Bandwidth
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2 nm
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Wavelength Accuracy
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< ± 1.0 nm
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0.0 to 4.0 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (plate)
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< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
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Photometric Accuracy (cuvette)
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< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
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Photometric Precision
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< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
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Photometric Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Light Source
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Xenon Flash Lamp
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Temperature Range
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Ambient + 4°C up to 45°C
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Temperature Uniformity (plate)
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± 0.5 °C @ 37°C
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Dimensions
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22 cm (h) x 58 cm (w) x 38 cm (d)
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Weight
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30lbs
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Photometric Analysis Modes
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Standalone
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Single wavelength absorbance or %Transmittance reading of the cuvette
(or test tube)
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Using SoftMax Pro
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Express data as Absorbance or %Transmittance
Single wavelength reading of microplate and/or cuvette
Multiple wavelength (up to six) reading of microplate and/
or cuvette
Kinetic and kinetic graphics of microplate and/or cuvette
Spectral scan (190–1000 nm) of microplate and/or cuvette
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Measurement Time (calibration off)
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Read Time (endpoint) Standard Read Microplate
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96 wells in 9 seconds (single wavelength)
96 wells in 9*N seconds (N wavelengths)
384 wells in 29 seconds (single wavelength)
384 wells in 29*N seconds (N wavelengths)
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Read Time (endpoint) Speed Read Microplate
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96 wells in 5 seconds (single wavelength)
96 wells in 5*N seconds (N wavelengths)
384 wells in 16 seconds (single wavelength)
384 wells in 16*N seconds (N wavelengths)
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Kinetic Read Intervals Microplate
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96 wells, 9-second minimum interval between readings (single
wavelength)
1 column, 2-second minimum interval between readings (single
wavelength)
384 wells, 29-second minimum interval between readings (single
wavelength)
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Read Time Cuvette
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1 second (single wavelength)
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Kinetic Read Intervals Cuvette
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2-second minimum interval between readings
(single wavelength)
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Scan Speed
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Cuvette: Normal Scan
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45*K nm/min (K = wavelength interval)
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Cuvette: Speed Scan
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130*K nm/min
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Microplate: Normal Scan
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33*K nm/min (8-well strip); 21*K nm/min (16-well strip)
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Microplate: Speed Scan
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135*K nm/min (8-well strip); 77*K nm/min (16-well strip)
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Temperature Regulation
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Reading Chamber
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Isothermal when temperature regulation is not enabled
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Range
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4°C above ambient to 45°C when temperature regulation enabled.
The ambient temperature must be >20°C to achieve temperature regulation
at 45°C.
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Resolution
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Our technicians decontaminate and clean equipment upon arrival at our facility and upon your purchase to create a neutral environment for your labs use.
Preliminary testing is then conducted on our equipment to identify potential defects. Upon completion, we replace all faulty and/or under-performing parts with original manufacturer parts that perform up to specification.
Any parts known to fail or malfunction on the piece of equipment are removed and replaced to insure the extended lifetime of your equipment.
Any necessary preventative maintenance is performed alongside repairs, so your purchase will be ready to go as soon as it arrives.
All of the electronics and boards are tested for proper function and response by our industry trained technicians. Any electronic deficiencies detected are immediately repaired.
Final testing cycles ensure your equipment performs fully at manufacturer specifications, guaranteeing you receive a properly working product ready for use.
Once we finish our rigorous testing and decontamination procedures, your order will be packaged to best protect it during shipment and will include delivery insurance.
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Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.
All Lab Equipment is Fully Recertified and Tested
Most Pieces Ship within 2 to 5 Business Days
All Products Come with a Standard Warranty
Trained Customer and Technical Support
Industry Leading Overall Customer Satisfaction
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Easy to find what you need with clear specs, website ordering was simple, equipment arrived quickly, very well packaged and working as expected! I couldn't ask for more, I'll definitely use them again!
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They do a very high quality job worth every penny they charge. Customer service is fantastic, they can always be reached by phone or email, and the staff are very knowledgeable and ready to help.
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