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Molecular Devices SpectraMax 384 Plus Microplate Reader
Molecular Devices SpectraMax 384 Plus Microplate Reader

Our Price: $8,695.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 1 to 3 Business Days
Included Documentation: Molecular Devices Absorbance Validation Report

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Quantity in Stock:1

Product Code: MD-SM384

Recertification Protocol
Technical Specification Sheet

Molecular Devices SpectraMax 384 Plus Microplate Reader

Photometric Range
0.0 to 4.0 OD

Wavelength Range
190 to 1000 nm

Wavelength Bandwidth
2 nm

  • Integrates the precision of a UV/Vis spectrophotometer with a microplate reader for fast and reliable data reporting.
  • Accommodates a wide range of sample types to ensure the needs of your laboratory are met.
  • Offers endpoint, kinetic, and spectral scan modes for versatile data analysis.
  • Revolutionizes microplate reading by normalizing well absorbance and correcting pipetting variations.
  • Accurately measures liquid depths and volumes down to 5µl in each microplate well.
  • Built-in linear and orbital shaking options for thorough sample mixing.

The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000 nm wavelength range. This 384 Plus comes from a long history of reliable tools built by Molecular Devices. This robust system is capable of reading 96 or 384 wells, each with its own sample beam and reference detector, for optical density measurement at a selected wavelength with endpoint (for a single point in time), kinetic (over a specific period of time), or spectral scan mode (over a specific wavelength range).

The high-precision nine channel system (8 for the microplate; 1 for the cuvette port) consists of sample beams and reference beams that mimic a dual beam spectrophotometer. The filter-less system includes monochromator, allowing for up to 6 wavelengths within the visible spectrum at a time, saving time without the need to change out filters.

The PathCheck sensor eliminates optical pathlength as a factor (and corrects pipetting variation), closing the gap between microplate readers and spectrophotometers. This is accomplished by PathCheck, in conjunction with the SoftMax Pro software, automatically normalizing well absorbance, allowing for the elimination of standard curves, and correcting volume differences in the wells. The 384 Plus measures liquid depth and volumes down to 5ul in each microplate well and scales the microplate data to standard 1 cm cuvette values. The monochromator selects wavelengths in 1nm increments, and the system is equipped with a built-in programmable linear and orbital plate shaking device.

Key applications include: DNA and RNA analysis; ELISAs and immunoassays; cell viability, proliferation, and cytotoxicity; endpoint analysis; enzyme kinetic assays; protein quantitation; endotoxin detection; and PathCheck.

The Molecular Devices SpectraMax 384 Plus Microplate Reader includes:
  • > Windows 10 Data Station with Pre-Loaded SoftMax Pro Software 6.5
  • > Interface Cable
  • > Molecular Devices Absorbance Validation Report
  • > Operator's Guide
  • > Power Cord
  • > Full 180 Day Parts and Labor Warranty

Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications

Spectral Range

190-1000 nm, tunable in 1 nm increments

Wavelength Range

190-1000 nm

Wavelength Selection

Monochromator, tunable 1.0nm increments

Speed Read Mode

Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds

Wavelength Bandwidth

2 nm

Wavelength Accuracy

< ± 1.0 nm

Wavelength Repeatability

± 0.2 nm

Photometric Range

0.0 to 4.0 OD

Photometric Resolution

0.001 OD

Photometric Accuracy (plate)

< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Accuracy (cuvette)

< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Precision

< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Baseline Flatness

< 0.001 OD

Stray Light

< 0.05% @ 230 nm

Light Source

Xenon Flash Lamp

Temperature Range

Ambient + 4C up to 45C

Temperature Uniformity (plate)

± 0.5C @ 37C


22 cm (h) x 58 cm (w) x 38 cm (d)



Photometric Analysis Modes


Single wavelength absorbance or %Transmittance reading of the cuvette (or test tube)

Using SoftMax Pro

Express data as Absorbance or %Transmittance

Single wavelength reading of microplate and/or cuvette

Multiple wavelength (up to six) reading of microplate and/

or cuvette

Kinetic and kinetic graphics of microplate and/or cuvette

Spectral scan (190 to 1000 nm) of microplate and/or cuvette

Measurement Time (calibration off)

Read Time (endpoint) Standard Read Microplate

96 wells in 9 seconds (single wavelength)

96 wells in 9*N seconds (N wavelengths)

384 wells in 29 seconds (single wavelength)

384 wells in 29*N seconds (N wavelengths)

Read Time (endpoint) Speed Read Microplate

96 wells in 5 seconds (single wavelength)

96 wells in 5*N seconds (N wavelengths)

384 wells in 16 seconds (single wavelength)

384 wells in 16*N seconds (N wavelengths)

Kinetic Read Intervals Microplate

96 wells, 9-second minimum interval between readings (single wavelength)

1 column, 2-second minimum interval between readings (single wavelength)

384 wells, 29-second minimum interval between readings (single wavelength)

Read Time Cuvette

1 second (single wavelength)

Kinetic Read Intervals Cuvette

2-second minimum interval between readings

(single wavelength)

Scan Speed

Cuvette: Normal Scan

45*K nm/min (K = wavelength interval)

Cuvette: Speed Scan

130*K nm/min

Microplate: Normal Scan

33*K nm/min (8-well strip); 21*K nm/min (16-well strip)

Microplate: Speed Scan

135*K nm/min (8-well strip); 77*K nm/min (16-well strip)

Temperature Regulation

Reading Chamber

Isothermal when temperature regulation is not enabled


4°C above ambient to 45°C when temperature regulation enabled.

The ambient temperature must be >20°C to achieve temperature regulation at 45°C.


± 0.1°C


± 1.0°C for microplate and cuvette chamber

Temperature Uniformity at Equilibrium

± 0.5°C at 37°C

Chamber Warm-Up Time

15 to 30 minutes (measured on air) after initiation of temperature regulation


± 0.2°C (regulated)

Computer Specifications




Intel Core i5-7200U CPU


16.0 GB

Hard Drive

240GB Internal SSD

Operating System

Windows 10 Professional, 64-bit


Why Buy From Marshall Scientific?

Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.

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Average Rating: Average Rating: 4.5 of 5 4.5 of 5 Total Reviews: 2 Write a review »

  4 of 4 people found the following review helpful:
4 of 5 Honest Company January 23, 2021
Reviewer: Mable Barton from Phoenix, AZ  
I would buy from Marshall Scientific again. They offer quality machines at great prices and are completely transparent.

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  7 of 7 people found the following review helpful:
5 of 5 System meets my needs November 9, 2020
Reviewer: Lee Alvarado from Henderson, Nevada  
Microplate reader works perfectly and interfaces without problems with the included laptop. System looks pretty clean and the laptop looks practically brand new.

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