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Molecular Devices SpectraMax 384 Plus Microplate Reader
Molecular Devices SpectraMax 384 Plus Microplate Reader
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Warranty: 90-Day Full Parts and Labor (Click for Info)
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Calibration: Molecular Devices Absorbance Validation Report

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Product Code: MD-SM384
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Description
Refurbishment Protocol
 		Technical Specification Sheet
 

Molecular Devices SpectraMax 384 Plus Microplate Reader


The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000 nm wavelength range. This 384 Plus comes from a long history of reliable tools built by Molecular Devices. This robust system is capable of reading 96 or 384 wells, each with its own sample beam and reference detector, for optical density measurement at a selected wavelength with endpoint (for a single point in time), kinetic (over a specific period of time), or spectral scan mode (over a specific wavelength range).

The high-precision nine channel system (8 for the microplate; 1 for the cuvette port) consists of sample beams and reference beams that mimic a dual beam spectrophotometer. The filter-less system includes monochromator, allowing for up to 6 wavelengths within the visible spectrum at a time, saving time without the need to change out filters.

The PathCheck sensor eliminates optical pathlength as a factor (and corrects pipetting variation), closing the gap between microplate readers and spectrophotometers. This is accomplished by PathCheck, in conjunction with the SoftMax Pro software, automatically normalizing well absorbance, allowing for the elimination of standard curves, and correcting volume differences in the wells. The 384 Plus measures liquid depth and volumes down to 5µl in each microplate well and scales the microplate data to standard 1 cm cuvette values. The monochromator selects wavelengths in 1nm increments, and the system is equipped with a built-in programmable linear and orbital plate shaking device.

Key applications include: DNA and RNA analysis; ELISAs and immunoassays; cell viability, proliferation, and cytotoxicity; endpoint analysis; enzyme kinetic assays; protein quantitation; endotoxin detection; and PathCheck.

Includes: > Windows 7 Data Station with Pre-Loaded SoftMax Pro Software 6.3.1 > Calibration Report > Power Cord > Full 90 Day Parts and Labor Warranty

Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications

Spectral Range

190-1000 nm, tunable in 1 nm increments

Wavelength Range

190-1000 nm

Wavelength Selection

Monochromator, tunable 1.0nm increments

Speed Read Mode

Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds

Wavelength Bandwidth

2 nm

Wavelength Accuracy

< ± 1.0 nm

Wavelength Repeatability

± 0.2 nm

Photometric Range

0.0 to 4.0 OD

Photometric Resolution

0.001 OD

Photometric Accuracy (plate)

< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Accuracy (cuvette)

< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Precision

< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Baseline Flatness

< 0.001 OD

Stray Light

< 0.05% @ 230 nm

Light Source

Xenon Flash Lamp

Temperature Range

Ambient + 4°C up to 45°C

Temperature Uniformity (plate)

± 0.5 °C @ 37°C

Dimensions

22 cm (h) x 58 cm (w) x 38 cm (d)

Weight

30lbs


Photometric Analysis Modes

Standalone

Single wavelength absorbance or %Transmittance reading of the cuvette (or test tube)

Using SoftMax Pro

Express data as Absorbance or %Transmittance

Single wavelength reading of microplate and/or cuvette

Multiple wavelength (up to six) reading of microplate and/

or cuvette

Kinetic and kinetic graphics of microplate and/or cuvette

Spectral scan (190–1000 nm) of microplate and/or cuvette


Measurement Time (calibration off)

Read Time (endpoint) Standard Read Microplate

96 wells in 9 seconds (single wavelength)

96 wells in 9*N seconds (N wavelengths)

384 wells in 29 seconds (single wavelength)

384 wells in 29*N seconds (N wavelengths)

Read Time (endpoint) Speed Read Microplate

96 wells in 5 seconds (single wavelength)

96 wells in 5*N seconds (N wavelengths)

384 wells in 16 seconds (single wavelength)

384 wells in 16*N seconds (N wavelengths)

Kinetic Read Intervals Microplate

96 wells, 9-second minimum interval between readings (single wavelength)

1 column, 2-second minimum interval between readings (single wavelength)

384 wells, 29-second minimum interval between readings (single wavelength)

Read Time Cuvette

1 second (single wavelength)

Kinetic Read Intervals Cuvette

2-second minimum interval between readings

(single wavelength)


Scan Speed

Cuvette: Normal Scan

45*K nm/min (K = wavelength interval)

Cuvette: Speed Scan

130*K nm/min

Microplate: Normal Scan

33*K nm/min (8-well strip); 21*K nm/min (16-well strip)

Microplate: Speed Scan

135*K nm/min (8-well strip); 77*K nm/min (16-well strip)


Temperature Regulation

Reading Chamber

Isothermal when temperature regulation is not enabled

Range

4°C above ambient to 45°C when temperature regulation enabled.

The ambient temperature must be >20°C to achieve temperature regulation at 45°C.

Resolution

± 0.1°C </



Spectramax 384 Plus Manual

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Preliminary testing is conducted to identify potential product defects, upon completion, we replace all faulty and/or under-performing parts with original manufacturer parts that perform up to specification.

Any parts known to fail or malfunction from excessive use are removed and replaced to insure the extended lifetime of your equipment.

Any necessary preventative maintenance is performed alongside repairs, so your purchase will be ready to go as soon as it arrives.

All electronics and boards are tested for proper function and response by our industry trained technicians. Any electronic deficiencies detected are immediately repaired.

Final testing cycles ensure your instrument performs fully at manufacturer specifications, guaranteeing you receive a properly working product ready for use.

Once we finish our rigorous testing and decontamination procedures, your order will be packaged to best protect it during shipment and will include delivery insurance.


Average Rating: 4.5 of 5 Total Reviews: 2 Write a review »

  0 of 0 people found the following review helpful:
 
System meets my needs August 29, 2018
Reviewer: Lee Alvarado from Henderson, Nevada  
Microplate reader works perfectly and interfaces without problems with the included laptop. System looks pretty clean and the laptop looks practically brand new.

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  0 of 0 people found the following review helpful:
 
Honest Company August 29, 2018
Reviewer: Mable Barton from Phoenix, AZ  
I would buy from Marshall Scientific again. They offer quality machines at great prices and are completely transparent.

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