Flow Channel Diameter
1.2 millimeters
Maximum Pressure
2 MPa, 20 bar, 290 psi
Valve type
Motorized rotary 8-way valve
Flow rate
Up to 100 milliliters per minute
Max Flow Rate
100 ml/min
Max Pressure
25 MPa (250 bar, 3600 psi)
Back Pressure
< 40 kPa at 100 ml/min with water
Leakage
<0.1 ul/min at 25 MPa
pH Stability Range
1-13, 1-14 (<1 day exposure)
Viscosity
Max 5 cP
Switch Time
< 260 ms between two adjacent positions
Operating Life Time
> 50,000 cycles, two adjacent positions
1.2 mm
<50 kPa at 100 ml/min with water
<260 ms between two adjacent positions
0.001 ‚ 100 ml/min
12 ml chamber: 10 MPa at 0.1 ‚ 100 ml/min (100 bar, 1450 psi)0.6, 2, and 5 ml chambers: 25 MPa at 0.001 ‚ 100 ml/min (250 bar, 3625 psi)90 µl and 0.2 ml chambers: 35 MPa at 0.001 ‚ 5 ml/min (350 bar, 5075 psi)
pH Range
1 ‚ 13, 1 ‚ 14 (< 1 day exposure)
Internal Volume
6 interchangeable mixing chambers: 90 µl, 0.2, 0.6, 2, 5, and 12 ml
1 static chamber and 1 dynamic chamber
95 fractions in 10-18 mm tubes
< 100 ml/min
pH Stability Long Term
1 ‚ 13
pH Stability Short Term
1 ‚ 14
Program Memory
99 programs
95 x 10-18 mm (Standard)
175 x 12 mm and 40 x 30 mm (Optional)
Time Mode
0.01 ‚ 9.999 min
Volume Mode
0.1 ‚ 999 ml
Voltage
100 ‚ 240 V
Flow Rate
Up to 50 ml
20 bar (2 MPa, 290 psi)
Operating Temperature
4° to 40°C
Dimensions (H x W x D)
158 x 148 x 99 mm
0.1 to 99999.99 ml
Height
480 mm
pH Stability range
1 to 13, 1 to 14 (<1 day exposure)
Operating Pressure Max.
50 bar (5 MPa) (725 psi)
1 µS/cm-999.9 mS/cm
2-12
260 x 370 x 100 mm
Accuracy
The greater of ± 2% of full scale or ± 10 µS/cm in the range 1 µS/cm-300 mS/cm ± 0.1 pH unit, temperature compensated Max. 0.1 pH units within 0‚10 ml/min (pH measurement)
Baseline noise
± 0.5% of full scale calibrated range
Drift
Max. 0.02 pH/h (measured at pH 4.0) ±0.5 °C per 10 h 0.1 pH unit deviation/10 h
Cell Constant
50 ± 20 cm-1
Response Time
Max. 10 s (0‚95% of step) (pH measurements) Max. 3 s (0‚95% of step) (Conductivity measurement)
100-120/220-240 VAC
Absorbance range
0.01 - 5 AU (full scale)
Autozero range
-0.2 - 2.0 AU
Baseline adjust Adjustable
0-100% of full scale
Wavelengths
Hg lamp, fixed: 254 and 280
by changing filter:313, 365, 405, 436 and 546 nm
Zn lamp: 214 nm
Linearity, 5 mm cell
<3% up to 2 AU at 254 nm
<5% up to 1 AU at 280 nm
Static noise
short term: 40x10-6 AU at 254 nm
(typically 6x10-6 AU at 254 nm)
long term: 40x10-6 AU at 254 nm
Static Drift
±100x10-6 AU/h at 254 nm
Flow Sensitivity
2x10-4 AU min/ml
Sample Capacity
Direct filling or 96 micro vials (96-well microplate)
Direct filling or 384 micro vials (384-well microplate)
48 vials (48-vial adapter)
Reproducibility
RSD ≤ 0.3% for flushed loop injections
RSD ≤ 0.5% for partial loopfill injections, injection volumes < 5 ul (5 ul sample, 100 ul sample loop, ≤ 2.5 MPa)
RSD ≤ 1.0% for ul pick-up injections, injection volumes > 5 ul
Memory Effect
0.1% with programmable needle wash
Cooling Capacity
Maximum of Ambient -12°C for 16°C < T < 40°C
Maximum Plate Height
Minimum Plate Height
Loop Volume
Dispenser Syringe
Dimensions (W x D x H)
11 x 15.7 x 17.3 in. (28 x 40 x 44 cm)
Weight
Electrical Requirements
0.5 to 5 ml/min (operating range)
10 ml/min (wash flow)
Flow Rate Accuracy
Flow rate ≤ 1 ml/min: ± 15%
Flow rate > 1 ml/min: ± 10%
Pressure Range
Pressure Accuracy
Viscosity Range
Gradient Flow Range
Wavelength Range
280 nm ± 3 nm single wavelength
Absorbance Range
Linearity
Flow Cell
Conductivity Range
Conductivity Accuracy
Number of Fractions
Fraction Volumes
Wavelength range
190‚700 nm in steps of 1 nm,
3 wavelengths simultaneously
Bandwidth
4 nm
Wavelength accuracy
±2 nm
Wavelength reproducibility
< 500 ms (one cycle from 214 nm to
254 nm and back to 214 nm)
< 2% deviation up to 2 AU at 260 nm with
Uracil at pH 2
short term: < 6x10-5 AU at 230 nm
long term: < 6x10-5 AU at 230 nm
< 2x10-4 AU/h at 254 nm
0.1mL/min to 50mL/min
Maximum Operating Pressure
1 MPa (145 psi)
Tubing ID (Flow Path)
0.75 mm
Single Wavelength Detection
Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436, and 546 nm (through manufacturer). Optional zink lamp with 214 nm filter (through manufacturer).
Flow Cell Path Length
2 mm (optional 5 mm through manufacturer)
Buffer Selection
2 inlets
Relative Humidity
20% to 95% (non-condensing)
2 to 12
Max 10 cP (< 10 ml/min), max 5 cP (>10 ml/min)
Dimensions (W x H x D)
400 x 530 x 250 mm
Pump P-920
Flow rate range isocratic mode gradient mode
0.05‚20 ml/min in steps of 10 µl/min 0.1‚20 ml/min in steps of 10 µl/min
Pressure range
0‚5 MPa (50 bar, 725 psi)
Pressure pulsation
Max. 6% (dP/P) during pump stroke
pH stability range
1‚13 (1‚14 < 1 day exposure)
< 10 ml/min
> 10 ml/min
Max. 10cP
Max. 5 cP
Flow rate reproducibility
flow rate 0.5‚10 ml/min
flow rate 10‚20 ml/min
rsd < 0.2%
rsd < 0.5%
Gradient composition
accuracy between turnings
accuracy during turnings
reproducibility
± 2% at 0.5‚5 ml/min and < 5 MPa
± 2% at 0.5‚5 ml/min and 0.5‚2.0 MPa
rsd < 0.5% at 0.5‚20 ml/min and < 5 MPa
< 0.5 µl/min (pump module A and B each)
P-900 Specifications
Flow Rate Range
Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min
Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min
Double mode: 0.01 to 200 ml/min in steps of 10 ul/min
0 to 10 MPa (100 bar, 1450 psi)
1 to 13 (1 to 14 with <1 day exposure)
ƒš± 2% or 20 ul/min, whichever is greater
Flow Rate Reproducibility
Flow rate ƒ‚°¥ 0.5 ml/min: rsd < 0.5%
Gradient Composition
Accuracy: < ƒš±1% at 0.05 to 10 ml/min
Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min
Pressure Sensor
Range: 0 to 27.5 MPa
Offset error: < 0.05 MPa
Scale error: < ƒš±2%
Total Volume Between Inlet and Outlet
< 800 ul/module
Isocratic mode: 0.001 to 10 ml/min in steps of 1 ul/min
Gradient mode: 0.001 to 10 ml/min in steps of 1 ul/min
Double mode: 0.001 to 20 ml/min in steps of 1 ul/min
0 to 25 MPa (100 bar, 1450 psi)
0.001 to 2 ml/min
190 to 700 nm
Solvent Compatibility
Peak Tubing Dimension
Isocratic mode: 0.001 to 10 ml/min in steps of 10 ul/min
Gradient mode: 0.001 to 10 ml/min in steps of 10 ul/min
Frac-950 Specifications
Fraction Size
0.1 to 99999.99 ml (volume mode)
0.1 to 99999.99 min (time mode)
Standard Mode
u-liter plates, DropSync: 0 to 1 ml/min
12 mm rack, DropSync: 0 to 1.5 ml/min
18 and 30 mm racks, DropSync: 0 to 2 ml/min
18 and 30 mm racks, Accumulator: 15 to 100 ml/min
Prep Mode
30 mm rack, Accumulator: 15 to 100 ml/min
Funnel-to-flask, Accumulator: 15 to 100 ml/min
250 ml bottles, Accumulator: 15 to 100 ml/min
Environmental Conditions
+4°C to +40°C
20 to 95% relative humidity
84 to 106 kPa (840 to 1060 mbar) atmospheric pressure
