GE AKTA Micro FPLC System

The GE AKTA Micro FPLC System is a highly efficient and versatile chromatography system designed for purifying proteins at micro-scale quantities, emphasizing on maximizing sample recovery and purity in research and development applications. This compact system is ideal for applications requiring precise control and flexibility, such as the purification of monoclonal antibodies, proteins, and nucleotides. The AKTA Micro FPLC's small footprint makes it an excellent choice for laboratories with limited space, while its user-friendly software ensures straightforward setup and operation for both experienced users and newcomers to chromatography. With its capability to handle small sample volumes with high precision, the AKTA Micro FPLC System is an essential tool for biochemists and molecular biologists focusing on the detailed study of biomolecules.

The GE AKTA Micro FPLC System is not only compact and user-friendly but also boasts advanced specifications tailored for precise and efficient chromatographic separations. The system is equipped with a P-905 Pump, offering a wide flow rate range from as low as 0.001 ml/min up to 2 ml/min, accommodating both isocratic and gradient modes, ensuring precise control over your purification process. With a flow rate accuracy of ± 2% or 2 µl/min (whichever is greater) and reproducibility with an RSD of less than 0.5%, the P-905 pump exemplifies reliability and precision, even at low flow rates. Its programmable pressure limits and internal volume of less than 600 µl/pump module make it versatile for various applications, supporting up to 35 MPa (5075 psi) pressure ranges.

The UV-900 Monitor enhances the system's capabilities with a broad wavelength range from 190 to 700 nm, allowing for the detection of multiple absorbances simultaneously, with exceptional accuracy and reproducibility. Its sensitivity and stability ensure accurate monitoring of your sample's purity throughout the process. Incorporating the pH/C-900 Monitor, the system can measure conductivity across a wide range from 1 µS/cm to 999.9 mS/cm, ensuring precise assessment of ionic content in your samples, critical for quality control and consistency in protein purification. The Frac-950 Fraction Collector rounds out this powerful system, designed for flexible fraction collection with a flow rate range of 0.001 to 100 ml/min. Its ability to handle a wide pH stability range ensures compatibility with a variety of samples, while its precise fraction size control and no spillage range make it an indispensable tool for collecting your purified compounds.

All of our equipment is fully tested to perform at factory specifications before being shipped.

GE AKTA Micro FPLC System Specifications

P-905 Pump Specifications
Flow Rate Range	Isocratic mode: 0.001 to 2 ml/min in steps of 1 ul/min Gradient mode: 0.04 to 2 ml/min in steps of 1 ul/min
Flow Rate Accuracy	± 2% or 2 ul/min, whichever is greater with compression compensation activated
Flow Rate Reproducibility	> 0.04 ml/min, RSD <0.5%
Increment	0.001 ml/min
Pressure Range	0 to 35 MPa (350 bar, 5075 psi)
Pressure Limits	Programmable upper and lower
Internal Volume	< 600 ul/pump module
Viscosity	Maximum: 3cP
Peak Tubing Dimensions	0.15 mm i.d.
UV-900 Monitor Specifications
Wavelength Range	190 to 700 nm in steps of 1 nm, three wavelengths simultaneously
Bandwidth	4 nm
Wavelength Accuaracy	± 2 nm
Wavelength Reproducibility	± 0.01 nm
Linearity	< 2% deviation up to 2 AU at 260 nm, with uracil at pH 2
Noise at 230 nm	< 6 x 10 -5 AU with 3 mm cell
Drift at 254 nm	< 2 x 10 -4 AU/h
Flow Cell Maximum Flow Rate	2 ml/min
Flow Cell Maximum Pressure	2 MPa (20 bar, 290 psi)
Flow Cell Path Length	3 mm
Flow Cell Internal Volume	0.7 ul
pH/C-900 Monitor Specifications
Conductivity Range	1 uS/cm to 999.9 mS/cm
Deviation from Theoretical Conductivity	Maximum ± 2% of full-scale calibration range or ± 10 uS/cm, whichever is greater in range
Noise	Maximum ± 0.5% of full-scale calibration range
Flow Cell Maximum Flow Rate	2 ml/min
Flow Cell Maximum Pressure	5 MPa (50 bar, 725 psi)
Flow Cell Internal Volume	Max 5 cP
Frac-950 Fraction Collector Specifications
Flow Rate Range	0.001 to 100 ml/min
pH Stability Range	1 to 3, 1 to 14 (> 1 day exposure)
Fraction Size	Volume mode: 0.04 to 99999.99 ml Time Mode: 0.04 to 99999.99 ml
No Spillage Range	Dropsync range: 0 to 3 ml/min Accumulator range: 15 to 100 ml/min

Computer Specifications
Model	Dell Optiplex 7010 Desktop PC
Processor	Intel Core i5-6500 Processor 3.6GHz
RAM	16GB
Hard Drive	250GB Internal SSD
Operating System	Windows 7 Professional, 64-bit

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