Operating Temperature Range
4°C to 40°C
Operating Humidity Range
< 95% non-condensing
Max Power Consumption
260 VA (210 W)
BTU
717
Analysis Run Time
30 to 45 min
Number of Samples
Up to 12 samples/chips
Sample Volume
1 µl
Sizing Range
50 to 7000 bp
Ambient Operating Temperature
5° to 40°C (41° to 104°F)
Electrical Requirements
100-240VAC, 50-60Hz
Power Consumption
60 VA
Dimensions (W x D x H)
162 x 412 x 290 mm (6.4 x 16.2 x 11.4 in)
Safety Standards: IEC, CSA, UL
Installation Category II, Pollution Degree 2
Detection Type
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
Pulse frequency
296 Hz for single signal mode 74 Hz for spectral mode
Excitation Monochromator
Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm
Reference System:
in-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition:
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
< 1.0 % At 200 nm, solution of 1.2% KCl, blank scan on air, 5 s integration
time; (EP*method)**
< 0.05 % At 220 nm, solution of 10 g/l NaI, blank scan on air, 5 s integration
time; (ASTM method)
< 0.03 % At 340 nm, solution of 50 g/l NaNO2, blank scan on air, 5 s integration
Setable Flow Range
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range
0.1 to 5 ml/min
Flow Precision
≤ 0.07% RSD or ≤ 0.02 min SD
Flow Accuracy
± 1% or 10 ul/min
Pressure
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Hydraulic System
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Recommended pH Range
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range
1 to 99% or 5 ul/min per channel
Composition Precision
≤ 0.5% absolute
Composition Accuracy
≤ 0.15% RSD at 1 ml/min
Max Flow Rate
10 ml/min per channel
Reduced performance above 5 ml/min
Number of Channels
4
Internal Volume
1 ml/channel
Materials in Contact with Solvent
PTE, PEEK
pH Range
1 – 14
Analog Output (AUX)
For pressure monitoring, range 0 – 3 V
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
100-120 or 220-240 VAC, 50/60 Hz
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
30 W
Line Voltage
100-120 220-240 VAC
Line Frequency
50 r 60 Hz
Weight
7kg (15.4 lbs)
13.5 x 17 x 3.1 in
