Home > Chromatography - HPLC >

Agilent 1260 Infinity I HPLC System w/ DAD & FLD
Agilent 1260 Infinity I HPLC System w/ DAD & FLD
 
 



Our Price: $48,995.00
Sale Price: $41,995.00
Money Saved: $7,000.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report

Request a Formal Quote


Quantity in Stock:1

Product Code: AG-1260HPLCDADFLD
Qty:

Description
Recertification Protocol
Technical Specification Sheet
 

Agilent 1260 Infinity I HPLC System w/ DAD & FLD

The Agilent 1260 Infinity I HPLC System w/ DAD & FLD offers the most flexibility for solvent selection and automation in HPLC method development, research and all HPLC applications requiring continuous access to a wide range of solvent choices. This system is ideally suited for multi-method, high-throughput workflows and offers convenient access to two solvents for isocratic or gradient analysis, for rapid method development and speeding up preparation of mobile phases and flushing the HPLC system.

The Agilent 1260 Infinity HPLC System's binary pump is comprises two identical pumps integrated into one housing. Binary gradients are created by high-pressure mixing. Typical applications are high throughput methods with fast gradients on high resolution 2.1 mm columns. The pump is capable of delivering flow in the range of 0.1 - 5 mL/min against up to 600 bar. Degassing of the solvents is done with the High Performance Degasser, which comprises four separate vacuum chambers with semipermeable tubings, a vacuum pump, and control assembly. The 1260 Infinity Autosampler is designed to offer the well-established flow-through design with variable volume injection and to achieve extremely low carryover, providing increased analysis speed with sensitivity, resolution, and precision. Paired with the autosampler is a thermostatted column compartment and ALS thermostat to serve as a temperature management system providing heating and cooling in order to meet extreme requirements of retention time reproducibility.

The featured Diode-Array Detector (DAD) is designed for the highest optical performance, GLP compliance, and easy maintenance. The DAD provides greatly enhanced sensitivity and baseline stability using a cartridge cell system with optofluidic waveguides. This detector provides for a wavelength range of 190 - 950 nm, a data acquisition rate of up to 80 Hz, and a programmable 1, 2, 4, 8, or 16 nm slit for rapid optimization of sensitivity, linearity, and spectral resolution. Also featured is the Fluorescence Detector (FLD), which brings high-sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. Some of the features of the FLD Detector include; a flash lamp for highest intensity and lowest detection limit, multi-wavelength mode for on-line spectra, spectra acquisition and simultaneous multi-signal detection, easy front access to the flow cell for fast replacement, and built-in wavelength accuracy verification. Users may also use an optional cuvette for off-line measurements.

The Agilent 1260 Infinity I HPLC System w/ DAD & FLD can be fully controlled from the included Agilent ChemStation Workstation Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.


This Agilent system is in excellent condition and will be fully tested to perform at factory specifications before being shipped.


INCLUDED:
The Agilent 1260 Infinity I HPLC System w/ DAD & FLD includes:
  • > G1312B Binary Pump
  • > G1329B Autosampler
  • > G1330B ALS Thermostat
  • > G1316C TCC
  • > G4225A HiP Degasser
  • > G1315D DAD Detector
  • > G1321B FLD Detector
  • > Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
  • > ChemStation Version 4.3 Software Installed
  • > Can/Crossover Cables
  • > Agilent Lan Interface Card Built-In
  • > Solvent Tubing
  • > 4 x Bottles, Caps and Solvent Filters
  • > New Stainless Steel Capillaries
  • > 1/4" Wrench & Mobile Phase Priming Syringe
  • > Waste Tubing
  • > All Power Cords
  • > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
  • > Full 180 Day Parts and Labor Warranty



Below is a List of Diagnostic Tests Performed On This System
G1315D Diode Array Detector & G1321B Fluorescence Detector Diagnostic Tests:
  • Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
  • Dark Current Test: The test measures the dark current from the detector optic.
  • Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
  • Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
  • Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
  • Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
G1312B Binary Pump Diagnostic Tests:
  • Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
  • Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
G1316C Column Compartment Diagnostic Tests:
  • Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
G1329B Autosampler Diagnostic Tests:
  • Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
System Tests:
  • 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1260 Infinity HPLC System.


Agilent 1260 Infinity I HPLC System w/ DAD & FLD Specifications

DAD Detector Specification
Detection Type 1024-element photodiode array
Light Source Deuterium and tungsten lamps
Wavelength Range 190 - 950 nm
Short Term Noise (ASTM) Single and Multi-Wavelength < ± 0.7 × 10^-5 AU at 254 nm and 750 nm
Drift < 0.9 × 10^-3 AU/hr at 254 nm
Linear Absorbance Range
> 2.0 AU (5%) at 265 nm
Wavelength Accuracy ± 1 nm
Wavelength Bunching 1 - 400 nm
Slit Width 1, 2, 4, 8, 16 nm / programmable slit
Diode Width < 1 nm
Signal Data Rate Up to 80 Hz
Spectra Data Rate Up to 80 Hz
Flow Cell

Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum

Control and Data Evaluation Agilent ChemStation for LC

FLD Detector

Specification

Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h

Pulse frequency

296 Hz for single signal mode 74 Hz for economy mode

Excitation Monochromator

Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Reference System

In-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Spectrum acquisition

Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All

Wavelength characteristic

Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz


Click Here to View More Specifications for the Agilent 1260 Infinity HPLC System

Binary Pump Specification
Hydraulic System Two dual piston in series pumps with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
Setable Flow Range 0.001 - 5 mL/min, in 0.001 mL/min increments
Flow Range

0.05 - 5.0 mL/min

Flow Precision < 0.07% RSD or < 0.02 min SD, whatever is greater
Flow Accuracy ± 1% or 10 µl/min, what ever is greater
Pressure Operating Range 0 - 60 MPa (0 - 600 bar, 0 - 8,700 psi) up to 5 mL/min
Pressure Pulsation < 2% amplitude or < 3 bar at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Compressibility Compensation Pre-defined, based on mobile phase compressibility
Recommended pH Range 1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient Formation High-pressure binary mixing
Delay Volume Standard delay volume configuration: 600 - 800 µl, (includes 400 µl mixer), dependent on back pressure

Low delay volume configuration: 120 µl
Autosampler Specification
Injection Range 0.1 - 100 µl in 0.1 µl increments
Precision < 0.25% from 5 - 40µl
< 0.5% from 2 - 5µl
< 0.7% from 1 - 2µl
< 1.5% from 0.5 - 1µl
Pressure Range Up to 600 bar (8,700 psi)
Sample Viscosity Range 0.2 - 5 cp
Sample Capacity 2 x well plates (MTP) + 10 x 2 ml vials
108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 ml vials
30 x 6 ml vials in 2 x 15 vial plate
100 Micro vial tray plus 10 additional 2 ml vials
54 Eppendorf tubes (0.5/1.5/2 ml) in 2 x 27 Eppendorf tube plates
Injection Cycle Time Typically < 21 seconds using default conditions and injection volume of 5µl
Carryover Typically < 0.004%
ALS Thermostat Specification
Temperature Range 4°C to 40°C in 1°C increments
Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50% 3°C to 8°C at a setpoint of 4°C
Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60% 3°C to 9°C at a setpoint of 4°C
Column Compartment Specification
Temperature Range Ambient -10°C to 80°C at flow rates up to 5 mL/min
Temperature Stability ± 0.15°C
Temperature Accuracy ± 0.8°C
Warm-Up/Cool-Down Time 5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
High Performance Degasser Specification
Number of Solvent Channels 4
Flow Range 0 - 10 mL/min per channel
Internal Volume Per Channel 0.45 mL per channel
Materials in Contact with Solvent TFE/PDD Copolymer, FEP, PEEK
pH Range 1 - 14


Computer Specifications

Model

Dell Optiplex 7010 Desktop PC

Processor

Intel Core i5-3470 Processor 3.2GHz

RAM

8GB

Hard Drive

250GB Internal SSD

Operating System

Windows 10 Professional, 64bit

Downloads

Why Buy From Marshall Scientific?

Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.

All Lab Equipment is Fully Recertified and Tested

Most Pieces Ship within 1 to 3 Business Days

All Products are Fully Warrantied

Trained Customer and Technical Support

Industry Leading Overall Customer Satisfaction

Bausch + Lomb
Working with Marshall Scientific was overall an enjoyable experience. Their customer service really helped me choose the product that fit my needs, and checking out was easier than I expected. Shipping was faster than expected which was also a plus!
James M. / Bausch + Lomb
Caltech
I have purchased a few items from Marshall Scientific over the years and I have always had great experiences with both the products and the customer support. Chat feature definitely makes both communicating and navigating easier. I would recommend this company to a colleague.
Lila B. / Caltech
Sanofi
I just received the two Polyscience chillers I ordered through your ecommerce store and I couldn't be happier with my purchase. Customer service was instrumental in helping me choose the correct model. Thanks again!
Nora F. / Sanofi
Ford
Working with Marshall Scientific has been an exceptional experience. Their team assisted me in selecting the perfect recirculating chiller for my research needs. Their expertise and guidance were invaluable, and I am grateful for their support. Marshall Scientific has been vital to the success of my research endeavors.
Zara K. / Ford
AWS
Collaborating with Marshall Scientific has been a truly exceptional experience. Their fast lead time for centrifuges is unparalleled, and their commitment to quality is evident in every aspect of their service. I would highly recommend them to anyone in need of a reliable and efficient scientific equipment supplier.
Mei L. / AWS
Pfizer
I recently ordered pipettes from Marshall Scientific and was impressed with their great selection and quality. The pipettes were exactly what I needed and worked perfectly. I would definitely order from them again.
Aarav S. / Pfizer
Mayo Clinic
The chat support I received while selecting a thermal cycler was exceptional. The representative was knowledgeable, patient, and helped me choose the perfect product for my needs. I am grateful for their assistance.
Anneliese M. / Mayo Clinic
University of Pennsylvania
I recently purchased a ChemiDoc imaging system from Marshall Scientific and was impressed with the great condition it arrived in. The shipping was professional and the system has been a valuable addition to my scientific research.
Tyler S. / University of Pennsylvania
Massachusetts Institute of Technology
The New Brunswick Incubator Shaker that I ordered from Marshall Scientific has just arrived and I cannot say enough how satisfied I am with my purchase. The shaker works like it came straight from the factory and the longer warranty is definitely a plus. I highly recommend Marshall to anyone looking for a company that stands behind their products.
Xiao L. / MIT
Mass General Hospital
My recently purchased microplate reader from Marshall Scientific has been a much needed addition to my lab. The reader is easy to use and provides accurate and reliable results. I have used it for various experiments including ELISA, cell viability assays, and protein quantification. The reader has greatly improved the efficiency and accuracy of my experiments. Thanks again!
Larisa R. / MGH
Tesla
I purchased a recirculating chiller from Marshall Scientific three months ago and couldn't be happier. The Thermoflex 2500 is a great unit and has been working perfectly. I actually bought three of them and they have exceeded my expectations.
Gavril K. / Tesla
Columbia University
I recently purchased a laboratory freezer from Marshall Scientific and I am very pleased with my purchase. The freezer is of high quality and has been perfect for storing my research samples. I use it mostly for cell culture and protein storage and it seems to do the job just fine. The overall cosmetic condition of the unit is perfect.
Juan M. / Columbia University
Rivian
I would like to extend a thanks to Kyle on the other side of Marshall Scientific's chat feature to help me select the right product for my laboratory needs. Kyle was knowledgeable and helped me choose the perfect equipment for my application. The chat feature was convenient and saved me time. I would definitely use it again in the future.
Yumi S. / Rivian
Quest Diagnostics
I recently received a Vicell cell counter from Marshall Scientific and I must say, I was anxious about making such a large purchase over the internet. However, the product arrived in perfect condition and has exceeded my expectations. I use it for various applications in my lab and it has been incredibly accurate and efficient. The professional shipping was also a plus. I will definitely be purchasing from Marshall Scientific again in the future.
Ethan S. / Quest Diagnostics
Click Here to View Our Other Testimonials

Share your knowledge of this product. Be the first to write a review »

Browse for more products in the same category as this item:

Chromatography - HPLC
Brand
Brand > Agilent
Chromatography Component
Chromatography Component > Systems