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Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader
Detection Modes
Abs (UV-Vis), FI, TRF, FP, Lumi
Microplate Formats
6-,12-,24-,48-,96-, 384-wells
Read Types
Endpoint, Kinetic, Spectrum, Well Scan
- Multi-mode plate reader capable of UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Luminescence
- Dual monochromator optics allowing a wide range of bioresearch and drug discovery applications without filter changes
- Suitable for various applications including ELISA, nucleic acid, protein assays, microbial growth, and more
- Features a Xenon Flash Lamp light source and Photomultiplier (R-3896) detector for high sensitivity and accuracy
- Compatible with multiple plate formats from 6 to 384 wells, providing flexibility for different assay requirements
- Includes temperature control from Ambient +4°C to 45°C with uniformity of ± 0.5°C at 37°C set point
- Features plate shaking with a timer from 0 to 999 seconds
The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP), Luminescence (Lumi).
Applications include endpoint, kinetic, spectrum, and multi-point well-scanning combining absorbance and fluorescence in 6-well to 384-well microplates, as well as endpoint, kinetic, and spectrum applications in absorbance and fluorescence using cuvettes, can be run with little to no optimization. For use within biochemistry, cell biology, immunology, molecular biology, and microbiology. Typical applications include ELISA, nucleic acid, protein, enzymatic type homogenous and heterogeneous assays, microbial growth, endotoxin testing, and pipettor calibration.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader includes:
- > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 6.5
- > Interface Cable
- > Molecular Devices Absorbance and Fluorescence Validation Report
- > Top Read Plate Adapter
- > Operator's Guide
- > Power Cord
- > Full Parts and Labor 180 Day Warranty
Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications
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UV/Vis Photometric Performance:
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Wavelength Range
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200 to 1000 nm
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Wavelength Selection
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Monochromator, tunable in 1.0 nm increments
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Wavelength Bandwidth
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≤ 4.0 nm
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Wavelength Accuracy
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± 2.0 nm
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0 to 4.0 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (Microplate)
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< ± 0.006 OD ± 1.0%, 0-2 OD
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Photometric Precision
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< ± 0.003 OD ± 1.0%, 0-2 OD
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Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Fluorescence Photometric:
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Dual Monochromators
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1 nm increments
EX: 250 to 850 nm
EM: 360 to 850 nm
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Bandwidth (EX, EM)
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9, 9 nm
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Detection Limit
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3.0 fmol/well FITC 200ul in 96 wells
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Time Resolved Fluorescence:
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Wavelength Range
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360 to 850 nm
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Sensitivity
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0.5fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
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Data Collection
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50 to 1450 usec increments
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Luminescence:
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Wavelength Range
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360 to 850 nm
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Detection Limit
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10 amol/well alkaline phosphatase 200 ul/well (obtained with Emerald II reagent from ABI)
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General Specifications:
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Light Source
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Xenon Flash Lamp
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Detector
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Photomultiplier (R-3896)
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Plate Formats
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6, 12, 24 , 48, 96, 384 wells
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Read Time
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96 well (Abs 18 sec, FI 15 sec)
384 well (Abs 49 sec, FI 45 sec)
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Shaker Time
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0 to 999 seconds
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Temperature Range
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Ambient +4C to 45C
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Temperature Uniformity
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± 0.5C @ 37C set point
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Electrical
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110 to 120 VAC, 50/60Hz
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- Confirm PCB And Power Supply Are Outputting Proper Voltages
- Inspect Display and Replace Any Dim/Dead Pixels
- Inspect Buttons and Confirm Full Functionality
- Check Functionality and Clearance of Plate Drawer
- Confirm Communication Between Plate Reader and Computer Software
- Inspect Internal Wiring and Electrical Connection
- Inspect Lamp and Replace as Needed
- Perform Appropriate Validation Protocol
- Perform All Current Updates for Data Station And Operating System
- Ensure Cosmetics Are Acceptable
To View More of Our Recertification Protocol Click Here
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Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.
All Lab Equipment is Fully Recertified and Tested
Most Pieces Ship within 1 to 3 Business Days
All Products are Fully Warrantied
Trained Customer and Technical Support
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