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Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader
Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader

Our Price: $18,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation Molecular Devices Absorbance and Fluorescence Validation Report

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Quantity in Stock:1

Product Code: MD-M5

Recertification Protocol
Technical Specification Sheet

Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader

Detection Modes
Abs (UV-Vis), FI, TRF, FP, Lumi

Microplate Formats
6-,12-,24-,48-,96-, 384-wells

Read Types
Endpoint, Kinetic, Spectrum, Well Scan

  • Multi-mode plate reader capable of UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Luminescence
  • Dual monochromator optics allowing a wide range of bioresearch and drug discovery applications without filter changes
  • Suitable for various applications including ELISA, nucleic acid, protein assays, microbial growth, and more
  • Features a Xenon Flash Lamp light source and Photomultiplier (R-3896) detector for high sensitivity and accuracy
  • Compatible with multiple plate formats from 6 to 384 wells, providing flexibility for different assay requirements
  • Includes temperature control from Ambient +4°C to 45°C with uniformity of ± 0.5°C at 37°C set point
  • Features plate shaking with a timer from 0 to 999 seconds

The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP), Luminescence (Lumi).

Applications include endpoint, kinetic, spectrum, and multi-point well-scanning combining absorbance and fluorescence in 6-well to 384-well microplates, as well as endpoint, kinetic, and spectrum applications in absorbance and fluorescence using cuvettes, can be run with little to no optimization. For use within biochemistry, cell biology, immunology, molecular biology, and microbiology. Typical applications include ELISA, nucleic acid, protein, enzymatic type homogenous and heterogeneous assays, microbial growth, endotoxin testing, and pipettor calibration.

All of our equipment is fully tested to perform at factory specifications before being shipped.

The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader includes:
  • > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 7.1.2
  • > Interface Cable
  • > Molecular Devices Absorbance and Fluorescence Validation Report
  • > Top Read Plate Adapter
  • > Operator's Guide
  • > Power Cord
  • > Full Parts and Labor 180 Day Warranty

Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications

UV/Vis Photometric Performance:

Wavelength Range

200 to 1000 nm

Wavelength Selection

Monochromator, tunable in 1.0 nm increments

Wavelength Bandwidth

≤ 4.0 nm

Wavelength Accuracy

± 2.0 nm

Wavelength Repeatability

± 0.2 nm

Photometric Range

0 to 4.0 OD

Photometric Resolution

0.001 OD

Photometric Accuracy (Microplate)

< ± 0.006 OD ± 1.0%, 0-2 OD

Photometric Precision

< ± 0.003 OD ± 1.0%, 0-2 OD

Baseline Flatness

< 0.001 OD

Stray Light

< 0.05% @ 230 nm

Fluorescence Photometric:

Dual Monochromators

1 nm increments

EX: 250 to 850 nm

EM: 360 to 850 nm

Bandwidth (EX, EM)

9, 9 nm

Detection Limit

3.0 fmol/well FITC 200ul in 96 wells

Time Resolved Fluorescence:

Wavelength Range

360 to 850 nm


0.5fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)

Data Collection

50 to 1450 usec increments


Wavelength Range

360 to 850 nm

Detection Limit

10 amol/well alkaline phosphatase 200 ul/well (obtained with Emerald II reagent from ABI)

General Specifications:

Light Source

Xenon Flash Lamp


Photomultiplier (R-3896)

Plate Formats

6, 12, 24 , 48, 96, 384 wells

Read Time

96 well (Abs 18 sec, FI 15 sec)

384 well (Abs 49 sec, FI 45 sec)

Shaker Time

0 to 999 seconds

Temperature Range

Ambient +4C to 45C

Temperature Uniformity

± 0.5C @ 37C set point


110 to 120 VAC, 50/60Hz

Why Buy From Marshall Scientific?

Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.

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