Agilent 1100 HPLC System with Diode Array Detector

The Agilent 1100 HPLC System guarantees the most efficient mixing and pulse-free solvent delivery for consistently fast and accurate results. The system’s optimized design using a quaternary pump allows variable stroke volumes for stable solvent flow. This electronically activated inlet valve runs synchronous to the pistons to eliminate vapor formation in volatile solvent, making the Agilent 1100 both remarkably easy-to-use and maintenance-free. It also features a high-speed proportioning valve and a pre-configured degasser, which allow convenient low-pressure mixing and a large solvent choice flexibility. The HPLC System’s vacuum degasser ensures reduced operating costs without the use of helium and optimum instrument performance.

The Agilent 1100 Diode Array Detector verifies separation quality with peak purity, multiple signals, and spectral libraries during your analytical quantification, offering an extended wavelength range with the highest sensitivity. The VWD and diode array detectors allow for automated wavelength verification for performance tracking. The Agilent 1100’s temperature management system ensures optimum baseline stability-even under rough ambient conditions. This detector features superb illumination using combined deuterium and tungsten lamps for the highest intensity and lowest detection limit from 190 to 950 nm, 1024 diodes and 1-nm slit for highest spectral resolution. The programmable slit helps you explore the spectral landscape for faster sample characterization, ensuring higher sensitivity or lower baseline noise as necessary on the 1100. Slit settings can be stored and reported from 1 – 16 nm with raw data for GLP traceability.

The unit can be operated with a hand-held controller or computer running Clarity software. The HPLC System features extensive diagnostics with compatible data analysis software for on-site and remote trouble-shooting.

This Agilent system is in excellent condition and will be fully tested to perform at factory specifications before being shipped.

Recertified Agilent 1100 System

Included with the Agilent 1100 HPLC System w/ DAD:

Includes:

> G1311A Quaternary Pump (Binary Pump Available)
> G1313A Autosampler
> G1315B DAD Detector
> G1316A Column Compartment
> G1322A Degasser (G1379A Degasser Available)
> Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
> Chemstation version 4.3 software installed
> Lab Advisor Software B.02.10
> Can/Crossover Cables
> Lan Interface Card
> Solvent Tubing
> Bottles, Caps and Solvent Filters
> Capillaries
> All Power Cords
> Full 90 Day Parts and Labor Warranty

Agilent 1100 HPLC System with DAD Detector Specifications

Type	Specification
Detection type	1024-element photodiode array
Light Source	Deuterium and tungsten lamps
Wavelength Range	190 - 950 nm
Short term noise (ASTM) Single and Multi-Wavelength	± 1 × 10-5AU at 254 and 750 nm
Drift	2 × 10-3AU/hr at 254 nm
Linear Absorbance range	> 2 AU (upper limit)
Wavelength accuracy	± 1 nm
Slit width	1, 2, 4 , 8, 16 nm
Diode Width	< 1 nm
Flow Cells	Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and Data Evaluation	Agilent ChemStation for LC
Analog Outputs	Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
Communications	Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and Maintenance	Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
GLP Features	Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
Housing	All Materials recyclable

Preventative Maintenance Performed on The Agilent 1100 HPLC System with DAD Detector

• Rotor Seal in the Injection Valve was Replaced
• Needle Seat was Replaced
• Autosampler Needle was Replaced
• PTFE Frit was replaced in Purge Valve
• Active Inlet Valve Cartridge was Replaced
• Quat Pump Seals were Replaced
• Old and Frayed LAN Cables were Replaced
• DAD Deuteriam Lamp was Tested and Replaced if Required
• Flow Cell was Flushed and Cleaned
• Internal Circuitry is Checked for Faulty/Shorted Components and Replaced Accordingly

List of tests performed on this Agilent 1100 HPLC System
G1315B Diode Array Detector Diagnostic Tests
Filter Test	The test moves the filter into the light path and measures the relative absorbance in the filter position.
Dark Current Test	The test measures the dark current from the detector optic.
Holmium Oxide Test	The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
Lamp Intensity Test	The test verifies functionality of the UV lamp at various wavelengths
Wavelength Intensity Test	This test confirms the accuracy of wavelengths measured.
Baseline Noise Test	This test measures the amount of baseline noise drift identified by the detector.
G1311A Quaternary Pump Diagnostic Tests
Pressure Test	The pressure test is a quick, built-in test designed to demonstrate the pressure tight-ness (up to 400 bar) of the system.
Leak Test	The leak test is a built in troubleshooting designed to demonstrate the leak-tightness of the pump.
G1316A Column Compartment Diagnostic Tests
Thermostat Test	Evaluates the cooling and heating performance of the two Peltier elements
G1313A Autosampler Diagnostic Tests
Injector Steps	Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
System Tests
4 Peak Test (Isocratic Standard)	Standard test published by Agilent to confirm complete functionality of the 1100 HPLC System.

Computer Specifications
Model	Lenovo ThinkCentre M90
Processor	Intel Core I5-650 Processor 3.2GHz
Ram	8GB
Hard Drive	500GB Internal HDD
Operating System	Windows 10 Professional, 64bit

Agilent 1100 Operators Manual