Agilent 1100 HPLC System with Diode Array Detector
The Agilent 1100 HPLC System guarantees the most efficient
mixing and pulse-free solvent delivery for consistently fast and accurate
results. The system’s optimized design using a quaternary pump allows variable
stroke volumes for stable solvent flow. This electronically activated inlet
valve runs synchronous to the pistons to eliminate vapor formation in volatile
solvent, making the Agilent 1100 both remarkably easy-to-use and
maintenance-free. It also features a high-speed proportioning valve and a
pre-configured degasser, which allow convenient low-pressure mixing and a large
solvent choice flexibility. The HPLC System’s vacuum degasser ensures reduced
operating costs without the use of helium and optimum instrument performance.
The Agilent 1100 Diode Array Detector verifies separation
quality with peak purity, multiple signals, and spectral libraries during your
analytical quantification, offering an extended wavelength range with the
highest sensitivity. The VWD and diode array detectors allow for automated
wavelength verification for performance tracking. The Agilent 1100’s
temperature management system ensures optimum baseline stability-even under
rough ambient conditions. This detector features superb illumination using
combined deuterium and tungsten lamps for the highest intensity and lowest
detection limit from 190 to 950 nm, 1024 diodes and 1-nm slit for highest
spectral resolution. The programmable slit helps you explore the spectral
landscape for faster sample characterization, ensuring higher sensitivity or
lower baseline noise as necessary on the 1100. Slit settings can be stored and
reported from 1 – 16 nm with raw data for GLP traceability.
The unit can be operated with a hand-held controller or
computer running Clarity software. The HPLC System features extensive
diagnostics with compatible data analysis software for on-site and remote
trouble-shooting.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
Recertified Agilent 1100 System
Included with the Agilent 1100 HPLC System w/ DAD:
Includes:
> G1311A Quaternary Pump (Binary Pump Available)
> G1313A Autosampler
> G1315B DAD Detector
> G1316A Column Compartment
> G1322A Degasser (G1379A Degasser Available)
> Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
> Chemstation version 4.3 software installed
> Lab Advisor Software B.02.10
> Can/Crossover Cables
> Lan Interface Card
> Solvent Tubing
> Bottles, Caps and Solvent Filters
> Capillaries
> All Power Cords
> Full 90 Day Parts and Labor Warranty
Agilent 1100 HPLC System with DAD Detector Specifications
Type
|
Specification
|
Detection type
|
1024-element photodiode array
|
Light Source
|
Deuterium and tungsten lamps
|
Wavelength Range
|
190 - 950 nm
|
Short term noise (ASTM) Single
and Multi-Wavelength
|
± 1 × 10-5AU at 254 and 750 nm
|
Drift
|
2 × 10-3AU/hr at 254 nm
|
Linear Absorbance range
|
> 2 AU (upper limit)
|
Wavelength accuracy
|
± 1 nm
|
Slit width
|
1, 2, 4 , 8, 16 nm
|
Diode Width
|
< 1 nm
|
Flow Cells
|
Standard: 13 µl volume, 10 mm cell
path length and 120 bar (1760 psi)
pressure maximum
Semi-Micro: 5 µl volume, 6 mm cell
path length and 120 bar (1760 psi)
pressure maximum
High pressure: 1.7 µl volume, 6 mm cell
path length and 400 bar (5880 psi)
pressure maximum
500 nano: 0.5 µl volume, 10 mm cell
path length and 50 bar (725 psi)
pressure maximum
|
Control and Data Evaluation
|
Agilent ChemStation for LC
|
Analog Outputs
|
Recorder/integrator: 100 mV or 1 V,
output range 0.001 – 2 AU, two
outputs
|
Communications
|
Controller-area network (CAN), GPIB,
RS-232C, APG Remote: ready, start,
stop and shut-down signals, LAN
optional
|
Safety and Maintenance
|
Extensive diagnostics, error detection
and display (through control module
and ChemStation), leak detection, safe
leak handling, leak output signal for
shutdown of pumping system. Low
voltages in major maintenance areas.
|
GLP Features
|
Early maintenance feedback (EMF) for
continuous tracking of instrument
usage in terms of lamp burn time with
user-settable limits and feedback
messages. Electronic records of
maintenance and errors. Verification of
wavelength accuracy with built-in
holmium oxide filter.
|
Housing
|
All Materials recyclable
|
- Rotor Seal in the Injection Valve was Replaced
- Needle Seat was Replaced
- Autosampler Needle was Replaced
- PTFE Frit was replaced in Purge Valve
- Active Inlet Valve Cartridge was Replaced
- Quat Pump Seals were Replaced
- Old and Frayed LAN Cables were Replaced
- DAD Deuteriam Lamp was Tested and Replaced if Required
- Flow Cell was Flushed and Cleaned
- Internal Circuitry is Checked for Faulty/Shorted Components and Replaced Accordingly
List of tests performed on this
Agilent 1100 HPLC System
|
G1315B Diode Array Detector Diagnostic
Tests
|
Filter Test
|
The test moves the filter into the light path and measures the
relative absorbance in the filter position.
|
Dark Current Test
|
The test measures the dark current from the detector optic.
|
Holmium Oxide Test
|
The test measures the Holmium spectrum from the built-in Holmium
filter. The spectrum is evaluated for peaks at different wavelengths.
|
Lamp Intensity Test
|
The test verifies functionality of the UV lamp at various wavelengths
|
Wavelength Intensity Test
|
This test confirms the accuracy of wavelengths measured.
|
Baseline Noise Test
|
This test measures the amount of baseline noise drift identified by
the detector.
|
G1311A Quaternary Pump Diagnostic Tests
|
Pressure Test
|
The pressure test is a quick, built-in test designed to demonstrate the
pressure tight-ness (up to 400 bar) of the system.
|
Leak Test
|
The leak test is a built in troubleshooting designed to demonstrate the
leak-tightness of the pump.
|
G1316A Column Compartment Diagnostic Tests
|
Thermostat Test
|
Evaluates the cooling and heating performance of the two Peltier
elements
|
G1313A Autosampler Diagnostic Tests
|
Injector Steps
|
Performs each movement of the sampling sequence under manual control.
This is useful for confirming autosampler functionality.
|
System Tests
|
4 Peak Test (Isocratic Standard)
|
Standard test published by Agilent to confirm complete functionality of the 1100 HPLC System.
|
Computer Specifications
|
Model
|
Lenovo ThinkCentre M90
|
Processor
|
Intel Core I5-650 Processor 3.2GHz
|
Ram
|
8GB
|
Hard Drive
|
500GB Internal HDD
|
Operating System
|
Windows 10 Professional, 64bit
|
Agilent 1100 Operators Manual