Operating Temperature Range
4°C to 40°C
Operating Humidity Range
< 95% non-condensing
Max Power Consumption
260 VA (210 W)
BTU
717
Temperature Range
4° to 40°C
Ambient Temperature
Dimensions
140 x 345 x 435 mm
Line Voltage
100-120 or 220-240 VAC
Line Frequency
50/60 Hz
Range 10° below ambient to 80° C
Temperature Stability
± 0.15°C
Temperature Accuracy
± 0.8°C
Column Capacity
Three 30cm columns
Warm-up/Cool Down
5 mins from ambient to 40°C
Type
Specification
Weight
0.8 kg
Dimensions (width ƒ” depth ƒ” height)
130 ƒ” 225 ƒ” 35 mm (5.1 ƒ” 8.9 ƒ” 1.4 inches)
Line voltage
22 VDC, ± 10 %
Power consumption
6 W / 20.5 BTU/hour
Ambient operating temperature
0 ‚ 55 °C (32 ‚ 131 °F)
Ambient non-operating temperature
-40 ‚ 70 °C (-40 ‚ 158 °F)
Humidty
< 95%, at 25 ‚ 40 °C (77 ‚ 104 °F)
Operating Altitude
Up to 2000 m (6562 ft)
Non-operating altitude
Up to 4600 m (15092 ft)
Safety standards: IEC, CSA, UL, EN
Installation category II, pollution degree 2. For indoor use only. Research Use Only. Not for use in Diagnostic Procedures.
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
Ambient Operating Temperature
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
Power Consumption
30 W
100-120 220-240 VAC
50 r 60 Hz
7kg (15.4 lbs)
Dimensions (W x D x H)
13.5 x 17 x 3.1 in
Max Flow Rate
10 ml/min per channel
Reduced performance above 5 ml/min
Number of Channels
4
Internal Volume
1 ml/channel
Materials in Contact with Solvent
PTE, PEEK
pH Range
1 ‚ 14
Analog Output (AUX)
For pressure monitoring, range 0 ‚ 3 V
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
Electrical Requirements
100-120 or 220-240 VAC, 50/60 Hz
4ƒš° to 40ƒš°C
Temperature accuracy at ambient
temperatures < 25 ƒš°C and humidity < 50%
- 1ƒš°C to + 4 ƒš°C at a setpoint of 4 ƒš°C
temperatures > 25 ƒš°C and/or humidity > 50%
- 1ƒš°C to + 5 ƒš°C at a setpoint of 4 ƒš°C
Internal Volume per Channel
Typically 1 ml per channel
PTFE, FEP, PEEK
RS-232 Output
For diagnostic purposes
Typically 12 ml per channel
PTFE, PEEK
Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 50%)
3°C to 8°C at a setpoint of 4°C
Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 60%)
3°C to 9°C at a setpoint of 4°C
46 lbs. (20.7 kg)
1 to 14
Hydraulic System
Two dual piston in series, with proprietary servo-controlled variable stroke drive, floating piston, active inlet valve, solvent selection valve and electronic flow control for flow rates up to 100 µl/min
Settable Column Flow Range
0.01 ‚ 20 µl/min
0.01 ‚ 100 µl/min (with the extended flow range kit)
0.001 ‚ 2.5 µl/min (with the electronic flow control bypassed)
Recommended Column Flow Range
1 ‚ 20 µl/min
10 ‚ 100 µl/min (with extended flow range kit)
0.1 ‚ 2.5 ml/min (with the electronic flow sensor bypassed)
Column Flow Precision
< 0.7 % RSD or 0.03 % SD (typically 0.4 % RSD or 0.02 % SD), at 10 µl/min and 50 µl/min column flow (based on RT, default setting)
Optimum Composition Range
1 to 99% or 5 µl/min per channel (primary flow), whatever is greater
Composition Precision
< 0.2 % SD, at 10 µl/min (20 µl flow sensor), 50 µl/min (100 µl flow sensor) and 1 ml/min (normal mode) default setting
Delay Volume
Typically 3 µl from the electronic flow control to the pump outlet for flow rates up to 20 µl/min.
Typically 12 µl from the electronic flow control to the pump outlet for flow rates up to 100 µl.
for flow rates up to 100 µl/min and electronic flow control active: primary flow path 180 - 480 µl without mixer, 600 - 900 µl with mixer (system pressure dependant)
Typically 180 to 480 µl (system pressure dependent) without mixer for flow rates up to 2.5 ml/min. (Mixer delay volume 420 µl)
Pressure Range
20 to 400 bar (5880 psi) system pressure
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Recommended pH Range
1.0 ‚ 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries.
Control and Data Evaluation
Agilent ChemStation for LC
Analog Output
For pressure monitoring, 2 mV/bar, one output
Communications
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and Maintenance
Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling,
leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
GLP Features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors.
Housing
All materials recyclable.
14.2 kg (31.3 lbs)
Dimensions (H x W x D)
200 x 345 x 435 mm (8 x 13.5 x 17 in)
Line frequency
100 ‚ 120 or 220 ‚ 240 VAC, ± 10 %
50 or 60 Hz, ± 5 %
Power consumption (apparent power)
Power consumption (active power)
300 VA max.
200 W max.
4 ‚ 55 °C (41 ‚ 131 °F)
40 to 70 °C (-4 to 158 °F)
Humidity
< 95 %, at 25 ‚ 40 °C (77 ‚ 104 °F) non-condensing
Tray
Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)
Settable injection volume
0.01 to 8 µl with small loop capillary
0.01 to 40 µl with extended loop capillary
Precision
Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl
Minimum sample volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials
Carryover
Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume
Sample viscosity range
0.2 - 5 cp
Recommended pH-range
1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries
Material in contact with solvent
Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel
GLP features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
All materials are recyclable
± 0.75 ƒ” 10-5AU at 254 nm
3 ƒ” 10-4AU/hr at 254 nm
Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximumHigh pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximumMicro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximumSemimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
± 0.05°C
Three 30 cm columns
Warm-Up / Cool-Down Time
5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
Dead Volume
1.6 µl low dispersion heat exchanger
3 µl heat left heat exchanger
6 µl right heat exchanger
Setable Flow Range
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range
0.1 to 5 ml/min
Flow Precision
°¤ 0.07% RSD or °¤ 0.02 min SD
Flow Accuracy
± 1% or 10 ul/min
Pressure
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range
1 to 99% or 5 ul/min per channel
°¤ 0.5% absolute
Composition Accuracy
°¤ 0.15% RSD at 1 ml/min
0.001 ‚ 10 ml/min, in 0.001 ml/min increments
0.2 ‚ 10.0 ml/min
< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min
Operating range 0 ‚ 40 MPa (0 ‚ 400 bar, 0 ‚ 5880 psi) up to 5 ml/min
Operating range 0 ‚ 20 MPa (0 ‚ 200 bar, 0 ‚ 2950 psi) up to 10 ml/min
< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)
1.0 ‚ 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay volume 800 ‚ 1100 µl, dependent on back pressure
0 ‚ 95% or 5 ‚ 100%, user selectable
< 0.2% SD, at 0.2 and 1 ml/min
5.5 x 13.5 x 17 in
100-120 or 22-240 VAC, 50/60 Hz, 220 VA
Detection Type
1024-element photodiode array
Light Source
Deuterium and tungsten lamps
Wavelength Range
190 ‚ 950 nm
Short Term Noise (ASTM) Single and Multi-Wavelength
± 1 ƒ” 10-5AU at 254 and 750 nm
Drift
2 ƒ” 10-3AU/hr at 254 nm
Linear Absorbance Range
> 2 AU (upper limit)
Wavelength Accuracy
± 1 nm
Wavelength Bunching
1 ‚ 400 nm
Slit Width
1, 2, 4, 8, 16 nm
Diode Width
< 1 nm
Flow Cells
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum
500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Analog Outputs
Recorder/integrator: 100 mV or 1 V, output range 0.001 ‚ 2 AU, two outputs
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter
Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features
Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range
0.1 ‚ 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
Replicate Injections
1 ‚ 99 from one vial
Typically < 0.5 % RSD of peak areas from 5 ‚ 100 µl
Typically < 1 % RSD of peak areas from 1 ‚ 5 µl
Minimum Sample Volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Typically < 0.1 %, < 0.05 % with external needle cleaning
Sample Viscosity Range
0.2 ‚ 50 cp
Replicate Injections Per Vial
1 ‚ 99
Sample Capacity
100 ƒ” 2-ml vials in 1 tray
40 ƒ” 2-ml vials in ½ tray
15 ƒ” 6-ml vials in ½ tray (Agilent vials only)
Injection Cycle Time
Typically 50 s depending on draw speed and injection volume
Early maintainece feedback (EMF), electronic records of maintainence and errors
Controller-area network (CAN). RS232C, APG remote standard, optional four external contact closures and BCD vial number output
Leak detection and safe leak handling, low voltages in maintence areas, error detection and display
Typically < 0.5 % RSD of peak areas from 5 ‚ 100 µl, Typically < 1 % RSD from 1 ‚ 5 µl
0.2 ‚ 5 cp
2 ƒ” well-plates (MTP) + 10 ƒ” 2 ml vials 100 x 2 ml in one tray 40 x 2 ml in half tray
Typically < 30 s using following standard conditions: Default draw speed: 200 µl/min Default eject speed: 200 µl/min Injection volume: 5 µl
Carry-Over
Typically < 0.01 % using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 µm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 µl caffeine (1 mg/ml), 5 µl water to test carryover Outside wash of needle before injection: 20 sec with water using flush port
0.1 ‚ 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification
required)
Detection type
Wavelength range
Short term noise (ASTM) single and multi-Wavelength
± 1 ƒ” 10-5 AU at 254 and 750 nm
2 ƒ” 10-3 AU/hr at 254 nm
Linear absorbance
range
Wavelength bunching
Slit width
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and data evaluation
Analog outputs
Safety and maintainence
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
All materials recyclable
< 1.0 % At 200 nm, solution of 1.2% KCl, blank scan on air, 5 s integration
time; (EP*method)**
< 0.05 % At 220 nm, solution of 10 g/l NaI, blank scan on air, 5 s integration
time; (ASTM method)
< 0.03 % At 340 nm, solution of 50 g/l NaNO2, blank scan on air, 5 s integration
0.001 ƒš‚Å“ 10 ml/min, in 0.001 ml/min increments
0.2 ƒš‚Å“ 10.0 ml/min
ƒš£ 0.07% RSD, or ƒš£ 0.02 min SD whatever is greater, based on retention time at constant room temperature
ƒš± 1% or 10 ƒšµl/min whatever is greater
Operating range 0 ƒš‚Å“ 40 MPa (0 ƒš‚Å“ 400 bar, 0 ƒš‚Å“ 5880 psi) up to 5 ml/min
Operating range 0 ƒš‚Å“ 20 MPa (0 ƒš‚Å“ 200 bar, 0 ƒš‚Å“ 2950 psi) up to 10 ml/min
1.0 ƒš‚Å“ 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Delay volume 800 ƒš‚Å“ 1100 ƒšµl, dependent on back pressure
0 ƒš‚Å“ 95% or 5 ƒš‚Å“ 100%, user selectable
