Operating Temperature Range
4°C to 40°C
Operating Humidity Range
< 95% non-condensing
Max Power Consumption
260 VA (210 W)
BTU
717
Temperature Range
4° to 40°C
Ambient Temperature
Dimensions
140 x 345 x 435 mm
Line Voltage
100-120 or 220-240 VAC
Line Frequency
50/60 Hz
Type
Specification
Weight
0.8 kg
Dimensions (width × depth × height)
130 × 225 × 35 mm (5.1 × 8.9 × 1.4 inches)
Line voltage
22 VDC, ± 10 %
Power consumption
6 W / 20.5 BTU/hour
Ambient operating temperature
0 – 55 °C (32 – 131 °F)
Ambient non-operating temperature
-40 – 70 °C (-40 – 158 °F)
Humidty
< 95%, at 25 – 40 °C (77 – 104 °F)
Operating Altitude
Up to 2000 m (6562 ft)
Non-operating altitude
Up to 4600 m (15092 ft)
Safety standards: IEC, CSA, UL, EN
Installation category II, pollution degree 2. For indoor use only. Research Use Only. Not for use in Diagnostic Procedures.
Range 10° below ambient to 80° C
Temperature Stability
± 0.15°C
Temperature Accuracy
± 0.8°C
Column Capacity
Three 30cm columns
Warm-up/Cool Down
5 mins from ambient to 40°C
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
Ambient Operating Temperature
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
Power Consumption
30 W
100-120 220-240 VAC
50 r 60 Hz
7kg (15.4 lbs)
Dimensions (W x D x H)
13.5 x 17 x 3.1 in
Max Flow Rate
10 ml/min per channel
Reduced performance above 5 ml/min
Number of Channels
4
Internal Volume
1 ml/channel
Materials in Contact with Solvent
PTE, PEEK
pH Range
1 – 14
Analog Output (AUX)
For pressure monitoring, range 0 – 3 V
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
Electrical Requirements
100-120 or 220-240 VAC, 50/60 Hz
Temperature accuracy at ambient
temperatures < 25 °C and humidity < 50%
- 1°C to + 4 °C at a setpoint of 4 °C
temperatures > 25 °C and/or humidity > 50%
- 1°C to + 5 °C at a setpoint of 4 °C
Internal Volume per Channel
Typically 1 ml per channel
PTFE, FEP, PEEK
RS-232 Output
For diagnostic purposes
Typically 12 ml per channel
PTFE, PEEK
Setable Flow Range
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range
0.1 to 5 ml/min
Flow Precision
≤ 0.07% RSD or ≤ 0.02 min SD
Flow Accuracy
± 1% or 10 ul/min
Pressure
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Hydraulic System
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Recommended pH Range
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range
1 to 99% or 5 ul/min per channel
Composition Precision
≤ 0.5% absolute
Composition Accuracy
≤ 0.15% RSD at 1 ml/min
14.2 kg (31.3 lbs)
Dimensions (H x W x D)
200 x 345 x 435 mm (8 x 13.5 x 17 in)
Line frequency
100 – 120 or 220 – 240 VAC, ± 10 %
50 or 60 Hz, ± 5 %
Power consumption (apparent power)
Power consumption (active power)
300 VA max.
200 W max.
4 – 55 °C (41 – 131 °F)
40 to 70 °C (-4 to 158 °F)
Humidity
< 95 %, at 25 – 40 °C (77 – 104 °F) non-condensing
Tray
Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)
Settable injection volume
0.01 to 8 µl with small loop capillary
0.01 to 40 µl with extended loop capillary
Precision
Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl
Minimum sample volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials
Carryover
Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume
Sample viscosity range
0.2 - 5 cp
Recommended pH-range
1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries
Material in contact with solvent
Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel
GLP features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications
Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Housing
All materials are recyclable
± 0.75 × 10-5AU at 254 nm
3 × 10-4AU/hr at 254 nm
Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximumHigh pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximumMicro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximumSemimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
0.001 – 10 ml/min, in 0.001 ml/min increments
0.2 – 10.0 ml/min
< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min
Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min
Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min
< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)
1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay volume 800 – 1100 µl, dependent on back pressure
0 – 95% or 5 – 100%, user selectable
< 0.2% SD, at 0.2 and 1 ml/min
5.5 x 13.5 x 17 in
100-120 or 22-240 VAC, 50/60 Hz, 220 VA
GLP Features
Early maintainece feedback (EMF), electronic records of maintainence and errors
Controller-area network (CAN). RS232C, APG remote standard, optional four external contact closures and BCD vial number output
Safety Features
Leak detection and safe leak handling, low voltages in maintence areas, error detection and display
Injection Range
0.1 – 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
Typically < 0.5 % RSD of peak areas from 5 – 100 µl, Typically < 1 % RSD from 1 – 5 µl
Sample Viscosity Range
0.2 – 5 cp
Sample Capacity
2 × well-plates (MTP) + 10 × 2 ml vials 100 x 2 ml in one tray 40 x 2 ml in half tray
Injection Cycle Time
Typically < 30 s using following standard conditions: Default draw speed: 200 µl/min Default eject speed: 200 µl/min Injection volume: 5 µl
Carry-Over
Typically < 0.01 % using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 µm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 µl caffeine (1 mg/ml), 5 µl water to test carryover Outside wash of needle before injection: 20 sec with water using flush port
Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Replicate Injections
1 – 99 from one vial
Typically < 0.5 % RSD of peak areas from 5 – 100 µl
Typically < 1 % RSD of peak areas from 1 – 5 µl
Minimum Sample Volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Typically < 0.1 %, < 0.05 % with external needle cleaning
0.2 – 50 cp
Replicate Injections Per Vial
1 – 99
100 × 2-ml vials in 1 tray
40 × 2-ml vials in ½ tray
15 × 6-ml vials in ½ tray (Agilent vials only)
Typically 50 s depending on draw speed and injection volume
0.1 – 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification
required)
Detection type
1024-element photodiode array
Light Source
Deuterium and tungsten lamps
Wavelength range
190 – 950 nm
Short term noise (ASTM) single and multi-Wavelength
± 1 × 10-5 AU at 254 and 750 nm
Drift
2 × 10-3 AU/hr at 254 nm
Linear absorbance
range
> 2 AU (upper limit)
Wavelength Accuracy
± 1 nm
Wavelength bunching
1 – 400 nm
Slit width
1, 2, 4, 8, 16 nm
Diode Width
< 1 nm
Flow Cells
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and data evaluation
Agilent ChemStation for LC
Analog outputs
Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and maintainence
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
All materials recyclable
< 1.0 % At 200 nm, solution of 1.2% KCl, blank scan on air, 5 s integration
time; (EP*method)**
< 0.05 % At 220 nm, solution of 10 g/l NaI, blank scan on air, 5 s integration
time; (ASTM method)
< 0.03 % At 340 nm, solution of 50 g/l NaNO2, blank scan on air, 5 s integration
Detection Type
Wavelength Range
Short Term Noise (ASTM) Single and Multi-Wavelength
± 1 × 10^-5AU at 254 and 750 nm
2 × 10^-3AU/hr at 254 nm
Linear Absorbance Range
Wavelength Bunching
Slit Width
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum
500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and Data Evaluation
Analog Outputs
Safety and Maintenance
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter
All materials recyclable.
Typically < 0.25 % RSD from 5 – 100 µl, Typically < 1 % RSD from 1 – 5 µl
Pressure Range
400 bar (5800 psi)
2 × well-plates (MTP) + 10 × 2 ml vials 108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
Pulse frequency
296 Hz for single signal mode 74 Hz for spectral mode
Excitation Monochromator
Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm
Reference System:
in-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition:
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
Trigger Modes
Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
Operating Modes
Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
Test Tube/Plate Sizes
Minimum 48 mm to 100 mm maximum
Maximum Tube Volume
ca. 45 ml
100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
Delay Volumes [μl]
Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
Diverter valve: ~15
Diverter valve to needle: ~110
Needle: -~5
Delay Calibration Sensor
Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
Diverter Valve
3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
Maximum Capacity
3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
Interfaces
Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors
Analysis Run Time
30 to 45 min
Number of Samples
Up to 12 samples/chips
Sample Volume
1 µl
Sizing Range
50 to 7000 bp
5° to 40°C (41° to 104°F)
100-240VAC, 50-60Hz
60 VA
162 x 412 x 290 mm (6.4 x 16.2 x 11.4 in)
Safety Standards: IEC, CSA, UL
Installation Category II, Pollution Degree 2
Typically < 1 % RSD 1 – 5 µl variable volume