0 to 5 ml/min per channel
5 to 10 ml/min at reduced degassing performance
Type
Specification
Weight
0.8 kg
Dimensions (width x depth x height)
130 x 225 x 35 mm (5.1 x 8.9 x 1.4 inches)
Line voltage
22 VDC, ± 10 %
Power consumption
6 W / 20.5 BTU/hour
Ambient operating temperature
0 to 55C (32 to 131F)
Ambient non-operating temperature
-40 to 70C (-40 to 158F)
Humidty
< 95%, at 25 ‚ 40C (77 to 104F)
Operating Altitude
Up to 2000 m (6562 ft)
Non-operating altitude
Up to 4600 m (15092 ft)
Safety standards: IEC, CSA, UL, EN
Installation category II, pollution degree 2. For indoor use only. Research Use Only. Not for use in Diagnostic Procedures.
10 ml/min per channel
Reduced performance above 5 ml/min
5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
0.01 to 20 µl/min
0.01 to 100 µl/min (with the extended flow range kit)
0.001 to 2.5 µl/min (with the electronic flow control bypassed)
1 to 20 µl/min
10 to 100 µl/min (with extended flow range kit)
0.1 to 2.5 ml/min (with the electronic flow sensor bypassed)
14.2 kg (31.3 lbs)
Dimensions (H x W x D)
200 x 345 x 435 mm (8 x 13.5 x 17 in)
Line Voltage
Line frequency
100 to 120 or 220 to 240 VAC, ± 10 %
50 or 60 Hz, ± 5 %
Power consumption (apparent power)
Power consumption (active power)
300 VA max.
200 W max.
4 to 55C (41 to 131F)
40 to 70C (-4 to 158F)
Humidity
< 95 %, at 25 to 40C (77 to 104F) non-condensing
Tray
Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)
Settable injection volume
0.01 to 8 µl with small loop capillary
0.01 to 40 µl with extended loop capillary
Precision
Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl
Minimum sample volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials
Carryover
Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume
Sample viscosity range
0.2 - 5 cp
Recommended pH-range
1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries
Material in contact with solvent
Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel
GLP features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications
Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Housing
All materials are recyclable
Setable Flow Range
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range
0.1 to 5 ml/min
Flow Precision
< 0.07% RSD or < 0.02 min SD
Flow Accuracy
± 1% or 10 ul/min
Pressure
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Hydraulic System
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Recommended pH Range
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range
1 to 99% or 5 ul/min per channel
Composition Precision
< 0.5% absolute
Composition Accuracy
< 0.15% RSD at 1 ml/min
0.001 to 10 ml/min, in 0.001 ml/min increments
0.2 to 10.0 ml/min
< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min
Operating range 0 to 40 MPa (0 to 400 bar, 0 to 5880 psi) up to 5 ml/min
Operating range 0 to 20 MPa (0 to 200 bar, 0 to 2950 psi) up to 10 ml/min
< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)
1.0 to 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay volume 800 to 1100 µl, dependent on back pressure
0 to 95% or 5 to 100%, user selectable
< 0.2% SD, at 0.2 and 1 ml/min
5.5 x 13.5 x 17 in
Electrical Requirements
100 to 120 or 22 to 240 VAC, 50/60 Hz, 220 VA
10 fg Anthracene, Ex=250 nm, Em=400 nm
RAMAN (H2O) > 200 (FLF rev A)
RAMAN (H2O) > 400 (FLF rev >A)
Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 second responsetime)
Range: settable 200 - 700 nm and zero-order
Bandwidth: 20 nm (fixed)
Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
Range: settable 280 - 900 nm and zero-order
Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
Repeatability: ± 0.2 nm
Accuracy: ± 3 nm setting
