Injection Precision
<0.2 % RSD or SD <10 nL, whatever is greater
Injection Linearity
0.9999 in the range of 0.1 ‚ 100 µL
Pressure Range
Up to 800 bar
Sample Viscosity Range
0.2 to 5.0 cP
Sample Capacity
1H Drawer: up to 8 drawers and 16 positions; Shallow well plates (MTP)
2H Drawer: up to 4 drawers and 8 positions; MTP, deep well plates, vials, Eppendorf
3H Drawer: up to 2 drawers and 4 positions; MTP, deep well plates, vials up to 6 mL, Eppendorf
Injection Cycle Time
<10 s using following standard conditions:Default draw speed: 100 µL/minDefault eject speed: 400 µL/minInjection volume: 1 µL
Carryover
<0.003 % (30 ppm) Multisampler Standard andDual Needle<0.0009 % (9 ppm) Multisampler Multiwash
Multiwash
Outer needle wash and seat backflush for carryover reduction with up to 3 different solvents
Dimensions (H x W x D)
12.6 x 15.6 x 18.4 in. (32 x 39.6 x 46.8 cm)
Weight
48.5 lb. (22 kg.)
Electrical Requirements
100 - 240V, 50/60Hz, 180VA
GLP Features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications
Controller-area network (CAN), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features
Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range
0.1 ‚ 100 µl in 0.1 µl increments (recommended 1 µl increments)
Up to 1500 µl with multiple draw (hardware modification required)
Replicate Injections
1 ‚ 99 from one vial
Precision
Typically < 0.25 % RSD of peak areas from 5 to 100 µl
Typically < 1 % RSD of peak areas from 1 to 5 µl
Minimum Sample Volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Typically < 0.1 %, < 0.05 % with external needle cleaning
0.2 ‚ 50 cp
Metering Devices
Metering pump in high pressure flow path
100 ƒ” 2 ml vials in 1 tray
40 ƒ” 2 ml vials in ½ tray
15 ƒ” 6 ml vials in ½ tray (Agilent vials only)
50 seconds for draw speed 200 µl/min, ejection speed 200 µl/min, injection volume 5 µl
Direct filling or 96 micro vials (96-well microplate)
Direct filling or 384 micro vials (384-well microplate)
48 vials (48-vial adapter)
Reproducibility
RSD ≤ 0.3% for flushed loop injections
RSD ≤ 0.5% for partial loopfill injections, injection volumes < 5 ul (5 ul sample, 100 ul sample loop, ≤ 2.5 MPa)
RSD ≤ 1.0% for ul pick-up injections, injection volumes > 5 ul
Memory Effect
0.1% with programmable needle wash
Cooling Capacity
Maximum of Ambient -12°C for 16°C < T < 40°C
Maximum Plate Height
Minimum Plate Height
Loop Volume
Dispenser Syringe
Dimensions (W x D x H)
11 x 15.7 x 17.3 in. (28 x 40 x 44 cm)
Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
0.1 ƒš‚Å“ 100 ƒšµl in 0.1 ƒšµl increments Up to 1500 ƒšµl with multiple draw (hardware modification
required)
1 ƒš‚Å“ 99 from one vial
Typically < 0.5 % RSD of peak areas from 5 ƒš‚Å“ 100 ƒšµl
Typically < 1 % RSD 1 ƒš‚Å“ 5 ƒšµl variable volume
1 ƒšµl from 5 ƒšµl sample in 100 ƒšµl microvial, or 1 ƒšµl from 10 ƒšµl sample in 300 ƒšµl microvial
0.2 ƒš‚Å“ 50 cp
Replicate Injections Per Vial
1 ƒš‚Å“ 99
100 ƒÆ’‚ 2-ml vials in 1 tray
40 ƒÆ’‚ 2-ml vials in ƒš½ tray
15 ƒÆ’‚ 6-ml vials in ƒš½ tray (Agilent vials only)
Typically 50 s depending on draw speed and injection volume
Early maintainece feedback (EMF), electronic records of maintainence and errors
Controller-area network (CAN). RS232C, APG remote standard, optional four external contact closures and BCD vial number output
Leak detection and safe leak handling, low voltages in maintence areas, error detection and display
0.1 ‚ 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
Typically < 0.5 % RSD of peak areas from 5 ‚ 100 µl, Typically < 1 % RSD from 1 ‚ 5 µl
0.2 ‚ 5 cp
2 ƒ” well-plates (MTP) + 10 ƒ” 2 ml vials 100 x 2 ml in one tray 40 x 2 ml in half tray
Typically < 30 s using following standard conditions: Default draw speed: 200 µl/min Default eject speed: 200 µl/min Injection volume: 5 µl
Carry-Over
Typically < 0.01 % using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 µm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 µl caffeine (1 mg/ml), 5 µl water to test carryover Outside wash of needle before injection: 20 sec with water using flush port
14.2 kg (31.3 lbs)
200 x 345 x 435 mm (8 x 13.5 x 17 in)
Line Voltage
Line frequency
100 ‚ 120 or 220 ‚ 240 VAC, ± 10 %
50 or 60 Hz, ± 5 %
Power consumption (apparent power)
Power consumption (active power)
300 VA max.
200 W max.
Ambient operating temperature
Ambient non-operating temperature
4 ‚ 55 °C (41 ‚ 131 °F)
40 to 70 °C (-4 to 158 °F)
Humidity
< 95 %, at 25 ‚ 40 °C (77 ‚ 104 °F) non-condensing
Tray
Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)
Settable injection volume
0.01 to 8 µl with small loop capillary
0.01 to 40 µl with extended loop capillary
Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl
Minimum sample volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials
Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume
Sample viscosity range
0.2 - 5 cp
Recommended pH-range
1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries
Material in contact with solvent
Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel
GLP features
Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Housing
All materials are recyclable
Typically < 0.5 % RSD of peak areas from 5 ‚ 100 µl
Typically < 1 % RSD of peak areas from 1 ‚ 5 µl
1 ‚ 99
100 ƒ” 2-ml vials in 1 tray
40 ƒ” 2-ml vials in ½ tray
15 ƒ” 6-ml vials in ½ tray (Agilent vials only)
