Sample Size
1 microliter
Path Length
1mm (with auto-ranging to 0.2 mm)
Light Source
Xenon flash lamp
Detector Type
2048-element linear silicon CCD array
Wavelength Range
220 to 750 nm
Wavelength Accuracy
1 nm
Wavelength Resolution
3 nm (FWHM at Hg 546 nm)
Absorbance Precision
0.003 absorbance (1mm path)
Absorbance Range
0.02 to 75 (10 mm equivalent absorbance)
Detection Limit
2 ng/microliter (dsDNA)
Maximum Concentration
3700 ng/microliter (dsDNA)
Measurement Cycle Time
10 seconds
Dimensions
14 x 20 cm
340—850 nm
Wavelength Selection
Monochromator, tunable in 1-nm increments
Wavelength Bandwidth
≤ 2.0 nm full width half maximum
± 1.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm across all optical channels
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy/Linearity
< ± 1.0% and ± 0.006 OD, 0.0-2.0 OD
Photometric Precision
< ± 1.0% and ± 0.003 OD, 0.0-2.0 OD
Stray Light
< 0.05% @ 340 nm
Xenon Flash Lamp
Microplate Read Time – Endpoint
9 seconds
Microplate Read Time – Kinetic
9-second minimum interval
Temperature Range
Ambient + 4° to 45°C
Temperature Uniformity
±0.5 °C @ 37 °C well-to-well
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
9 nm
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Ambient + 4° up to 45°C
± 1° at 37°C
< 1.0 % At 200 nm, solution of 1.2% KCl, blank scan on air, 5 s integration
time; (EP*method)**
< 0.05 % At 220 nm, solution of 10 g/l NaI, blank scan on air, 5 s integration
time; (ASTM method)
< 0.03 % At 340 nm, solution of 50 g/l NaNO2, blank scan on air, 5 s integration
Spectral Range
190-1000 nm, tunable in 1 nm increments
190-1000 nm
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
2 nm
< ± 1.0 nm
± 0.2 nm
0.0 to 4.0 OD
Photometric Accuracy (plate)
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
< 0.05% @ 230 nm
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
≤ 4.0 nm
± 2.0 nm
0 to 4.0 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
Fluorescence Photometric Performance
Dual Monochromators
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Detector
Photomultiplier tube (PMT)
Read Time
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
