Wavelength Range:
Microplate Capacity
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence modes: plates up to 1.25” (31.75 mm) high
Environment
Operational temperature 18º to 40°C
Humidity
10% to 85% relative humidity (non-condensing)
Power Supply
24-volt external power supply compatible with
100-240 V~; +10% @50-60 Hz
Power Consumption
250W maximum
Incubation
Temperature control range from 4º over ambient to 50ºC.
Temperature variation ± 0.50°C across the plate @ 37°C (250 µl per well with the plate sealed)
Plate Shaking
Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user
Luminescence
50 amol/well flash ATP in a 96-well plate (low-noise #49984 PMT)
500 amol/well flash ATP in a 96-well plate (red-shifted #49721 PMT)
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth
9 nm
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Light Source
Xenon flash lamp
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
< 1.0 % At 200 nm, solution of 1.2% KCl, blank scan on air, 5 s integration
time; (EP*method)**
< 0.05 % At 220 nm, solution of 10 g/l NaI, blank scan on air, 5 s integration
time; (ASTM method)
< 0.03 % At 340 nm, solution of 50 g/l NaNO2, blank scan on air, 5 s integration
Spectral Range
190-1000 nm, tunable in 1 nm increments
Wavelength Range
190-1000 nm
Wavelength Selection
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
2 nm
Wavelength Accuracy
< ± 1.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0.0 to 4.0 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (plate)
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Xenon Flash Lamp
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
≤ 4.0 nm
± 2.0 nm
0 to 4.0 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
Fluorescence Photometric Performance
Dual Monochromators
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Detector
Photomultiplier tube (PMT)
Read Time
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
