Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth
9 nm
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Light Source
Xenon flash lamp
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
Absorbance Accuracy
3% (at 0.74 Abs at 350nm)
Absorbance Range
Pedestal: 0-300 ABS Cuvette: 0 - 1.5 ABS
Accuracy
0.002
Accuracy (Absorption)
3% at (0.74 at 350nm)
Compatibility
Microsoft™ Windows™ XP (32-bit) w/Service Pack (SP) 2 or later, Vista™ (32-bit), Windows 7 (32 bit and 64 bit), Windows 10 (64 bit)
Concentration
2ng/µl-15,000ng/µl (dsDNA) ng/µl
Detection Limits
Pedestal: 2ng/µL (dsDNA), 0.10mg/mL (BSA) Cuvette: 0.4ng/µL (dsDNA), 0.01 mg/mL (BSA)
Detection Range
Pedestal: 2-15,000ng/µL (dsDNA) , 0.10 - 400mg/mL (BSA)
Detector Type
2048-element linear silicon CCD array
Dimensions
14 x 20cm
Heating Range
37°; ±0.5°C
Lamp
Xenon Flash
Measurement Time
<3 sec.
No. of Samples
1
Pathlength (Metric)
10; 5; 2; 1mm
Power Consumption
12VDC, 5W
Sample Volume (Metric)
1000, 0.5-2.0µL
Spectral Resolution
=1.8nm (FWHM at Hg 253.7)
System Requirements
Microsoft Windows 7 Professional (32-bit and 64-bit), Windows 8 (32- and 64-bit), and Windows 10 Pro (64-bit)
Wavelength Accuracy
±1nm
Wavelength Range
190-840nm
Microplate Capacity
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence/Fluorescence modes: plates up to 1.25” (31.75 mm) high Absorbance mode: plates up to 0.8” (20.30 mm) high
Environment
Operational temperature 18º to 40°C
Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
Humidity
10% to 85% relative humidity (non-condensing)
100 VA max, 130 VA max with injectors
340—850 nm
Wavelength Selection
Monochromator, tunable in 1-nm increments
≤ 2.0 nm full width half maximum
± 1.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm across all optical channels
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy/Linearity
< ± 1.0% and ± 0.006 OD, 0.0-2.0 OD
Photometric Precision
< ± 1.0% and ± 0.003 OD, 0.0-2.0 OD
Stray Light
< 0.05% @ 340 nm
Xenon Flash Lamp
Microplate Read Time – Endpoint
9 seconds
Microplate Read Time – Kinetic
9-second minimum interval
Ambient + 4° to 45°C
±0.5 °C @ 37 °C well-to-well
< 1.0 % At 200 nm, solution of 1.2% KCl, blank scan on air, 5 s integration
time; (EP*method)**
< 0.05 % At 220 nm, solution of 10 g/l NaI, blank scan on air, 5 s integration
time; (ASTM method)
< 0.03 % At 340 nm, solution of 50 g/l NaNO2, blank scan on air, 5 s integration
Spectral Range
190-1000 nm, tunable in 1 nm increments
190-1000 nm
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
2 nm
< ± 1.0 nm
± 0.2 nm
0.0 to 4.0 OD
Photometric Accuracy (plate)
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
< 0.05% @ 230 nm
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
≤ 4.0 nm
± 2.0 nm
0 to 4.0 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
Fluorescence Photometric Performance
Dual Monochromators
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Detector
Photomultiplier tube (PMT)
Read Time
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
