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GE Biacore 3000 SPR Biomolecular Interaction Analyzer
GE Biacore 3000 SPR Biomolecular Interaction Analyzer
 
 
Our Price: $9,780.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 6 to 8 Business Days

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Description
Recertification Protocol
Technical Specification Sheet
 

GE Biacore 3000 SPR Biomolecular Interaction Analyzer


The GE Biacore 3000 SPR Biomolecular Interaction Analyzer is an established, label-free system for detailed studies of biomolecular reactions. The Biacore 3000 delivers comprehensive characterization of the interaction, answers questions about the rate constants, affinity, specificity, and determines the active concentration of components. The unique ability to recover and transfer interaction and further characterization makes the system highly applicable to fast identification of unknown interactants. This analyzer is suitable for studying the interactions of proteins, protein conjugates, nucleic acids, lipid micelles, and even larger particles such as viruses and whole cells. Other major applications include measurement of kinetic constants, detection of binding partners and patterns in screening applications, monitoring of enzymatic processes, defining potential drug targets, and more.

The GE Biacore 3000 SPR Biomolecular Interaction Analyzer is designed for real-time biomolecular interaction analysis using surface plasmon resonance (SPR) technology. This technique monitors the formation and dissociation of biomolecular complexes on a sensor surface as the interaction occurs. By covalently attaching one molecule (the ligand) to the surface, the binding of another molecule in a solution (the analyte) with the ligand is followed. Measurements are made under conditions of continuous flow, where the sensor surface forms one wall of the flow cell. The Biacore 3000 measures surface protein concentrations from a few pg/mm^2, and can monitor changes in the surface concentration on a time scale down to 0.1 seconds. This technology allows for determination of binding specificity, kinetics, and concentration with analytes ranging in size from about 150 to 10^6 g/mole (Daltons) or more. Operation of the Biacore 3000 can be preprogrammed for analysis of up to 192 samples ( 2 x 96-well microplates).


INCLUDED:
The GE Biacore 3000 SPR Biomolecular Interaction Analyzer includes:
  • > Windows Data Station
  • > Biacore 3000 Control Software Installed
  • > Autosampler Rack
  • > Power Cord
  • > Operator's Guide
  • > Full 180 Day Parts and Labor Warranty

GE Biacore 3000 SPR Biomolecular Analyzer Specifications

Detection Unit Specification
Light Source LED
Wavelength

760 nm

Band Width < 5 nm
Refractive Index Range 1.33 - 1.40 (0 - 70,000 RU)
Baseline Drift Typically < ± 0.3 RU/minute
Data Output

One Flow Cell

Two Flow Cells

Four Flow Cells


0.1, 1 or 10 measurements/s (0.1 - 10 Hz)

0.1, 1 or 5 measurements/s (0.1 - 2 Hz)

0.1, 1 or 2 measurements/s (0.1 - 5 Hz)
Noise Typically < 0.3 RU RMS (10 Hz)
< 0.1 RU RMS (1 Hz)
Temperature Range 4°C to 40°C (not lower than 20°C below ambient temperature)
Temperature Stability Better than ± 3 x 10^-3°C/min
Temperature Equilibration Time

After 5°C Step

After 10°C Step


Typically < 60 minutes

Typically < 120 minutes
Molecular Weight Detection > 180 Da (Daltons)
Pump Specification
Flow Rate Range 1 - 100 µl/minute user-controlled
Increment Steps of 1 µl/minute
Flow Rate Accuracy < ± 2%
Flow Rate Precision < ± 1%
Pressure Range < 0.2 MPa
Autosampler Specification
Dispensing Volume Range 5 - 500 µl (including air segment)
Increment Steps of 1 µl
Accuracy < 1% deviation for volumes greater than or equal to 50 µl
Precision

50 µl

5 µl


< 0.4% CV

< 2% CV
Carry Over Between Vials Typically < 1 µl without septum
Typically < 0.1 µl with septum
Sample Capacity per Rack

Microplate

Rack Type A



Rack Type B

Rack Type C


96-well (0.2 ml/well)

5 Vials, diam. 16 mm (4 ml)
12 Vials, diam. 9 mm (1.5 ml)
40 Vials, diam. 7 mm (0.7 ml)

60 Vials, diam. 9 mm (1.5 ml)

24 Vials, diam. 10.8 mm (Eppendorf 1.5/2.0 ml)
Maximum Vial Height 55 mm
Integrated µ-Fluidic Cartridge Specification
Number of Flow Cells 4
Valves Pneumatic Membrane Valves
Injection Volume to Flow Cell 5 - 750 µl, steps of 1 µl
Carry Over from Previous Injection < 0.1%
Sample Loop Capacity 120 µl (maximum injection volume of 100 µl)
General Specification
Ambient Temperature Range 15°C to 35°C
Ambient Humidity 20 to 85% RH
Dimensions (L x W x H) 29.9 x 13.8 x 24 in (760 x 350 x 610 mm)
Weight 110 lbs (50 kg)
Electrical Requirements 100-120V / 220-240V, 47-63 Hz, 580 VA


GE Biacore 3000 SPR Biomolecular Interaction Analyzer Manual

Click Here to View the Technical Specs for the GE Biacore 3000 SPR Analyzer

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