Cytometer

Dimensions (W x H x D)

91 x 64 x 61 cm (35.7 x 25.2 x 24 in)

Workspace Dimensions

Height (with flow cell access door open): 85 cm (33.5 in)

Power Requirements

100/115/230 VAC (50-60 Hz)

Fluorescence Threshold Sensitivities

FITC < 100 MESF

PE < 50 MESF

Forward and Side Scatter Sensitivities

Platelets can be resolved from noise

Forward Scatter Sensitivity

1 micron

Side Scatter Sensitivity

0.5 micron

Data Acquisition Channels

8 to 10 parameters: 6 to 8 fluorescent and 2 scatter parameters

Fluidics Cart

Dimensions (W x H x L)

79 x 64 x 61 cm (31.1 x 25.2 x 24 in)

BD FACSFlow Cubitainer Capacity

20 L

BD FACSClean Solution Cubitainer Capacity

5 L

BD FACS Shutdown Solution Cubitainer Capacity

5 L

Waste Tank Capacity

10 L

Performance

Fluorescence Sensitivity

FITC-A: fewer than 80 molecules of equivalent soluble fluorochrome (MESF-FITC) PE-A: fewer than 30 molecules of equivalent soluble fluorochrome (MESF-PE) Measurements performed using SPHERO™ Rainbow Calibration Particles RCP-30-5A

Fluorescence Resolution

Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN).

Fluorescence Linearity

Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser

Forward and Side Scatter Sensitivity

Enables separation of fixed platelets from noise.

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Data Acquisition Rate

Theoretical maximum electronic data collection of 70,000 events/second. Typical operation at 20,000 events/ second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min.