Dimensions (W x D x H)
11.5 x 17.5 x 11.5 in.
Weight
19.4 lbs
Operating Power
100–240 VAC, 2.5 A, 120 V, 50/60 Hz
Electrical Input
12 VDC, 13 A
Operating Temp
10 - 40°C
Operating Humidity
< 80% (non-condensing)
Processing Time
10 seconds to 2 minutes, depending on the number of fields captured
Sample Concentration Range
1 x 10^5 - 1 x 10^7 cells / mL
Particle/cell Diameter Range
5-60 µm
Required Sample Volume
25 µL
Firmware
Tali Image-Based Cytometer Firmware
USB
4 GB
Optics
3 channels (bright field, green fluorescence, red fluorescence)
Excitation
Green channel LED (458 ± 20 nm), Red channel LED (530 ± 20 nm)
Green Channel Filter
466/40 EX, 495 LP Di, 525/50 EM
Red Channel Filter
543/22 EX, 580 LP Di, 585 LP EM
Camera
1.3 Mega pixels, 4x objective, 4x or 16x digital zoom
Cytometer
Dimensions (W x H x D)
91 x 64 x 61 cm (35.7 x 25.2 x 24 in)
Workspace Dimensions
Height (with flow cell access door open): 85 cm (33.5 in)
Power Requirements
100/115/230 VAC (50-60 Hz)
Fluorescence Threshold Sensitivities
FITC < 100 MESF
PE < 50 MESF
Forward and Side Scatter Sensitivities
Platelets can be resolved from noise
Forward Scatter Sensitivity
1 micron
Side Scatter Sensitivity
0.5 micron
Data Acquisition Channels
8 to 10 parameters: 6 to 8 fluorescent and 2 scatter parameters
Fluidics Cart
Dimensions (W x H x L)
79 x 64 x 61 cm (31.1 x 25.2 x 24 in)
BD FACSFlow Cubitainer Capacity
20 L
BD FACSClean Solution Cubitainer Capacity
5 L
BD FACS Shutdown Solution Cubitainer Capacity
Waste Tank Capacity
10 L
Performance
Fluorescence Sensitivity
FITC-A: fewer than 80 molecules of equivalent soluble fluorochrome (MESF-FITC) PE-A: fewer than 30 molecules of equivalent soluble fluorochrome (MESF-PE) Measurements performed using SPHERO™ Rainbow Calibration Particles RCP-30-5A
Fluorescence Resolution
Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN).
Fluorescence Linearity
Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
Forward and Side Scatter Sensitivity
Enables separation of fixed platelets from noise.
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Data Acquisition Rate
Theoretical maximum electronic data collection of 70,000 events/second. Typical operation at 20,000 events/ second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min.
