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BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader
BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader

Our Price: $17,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation: ABS, FLD, LUM, and Self Test Calibration Report

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Quantity in Stock:1

Product Code: B-SH4

Recertification Protocol
Technical Specification Sheet

BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader

The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader is a compact, advanced multi-detection system built with patented Hybrid Technology, melding monochromator-based and filter-based fluorescence detection systems. This unique combination offers unparalleled sensitivity and versatility, accommodating a wide array of microplate-based assays both for present and future requirements. Key features include a broad spectrum of detection modes such as Fluorescence Intensity, Time-Resolved Fluorescence, Luminescence, UV-Visible Absorbance, and more. Additionally, its modular and upgradeable architecture allows for cost-effective customization, ensuring it meets evolving laboratory needs.

At its core, the Synergy H4 houses a quadruple mono system with two double-grating monochromators, facilitating superior spectral scanning performance and flexibility. The variable bandpass selection further enhances the system's performance. On the other hand, the filter/mirror system of the Synergy H4 optimizes energy delivery to the sample, ensuring high signal-to-noise ratios, faster read speeds, and precise control over optical parameters. The unit's compatibility with the Take3 Plate also enables low-volume nucleic acid quantification, making the Synergy H4 an invaluable tool for achieving precise and efficient results in a variety of biochemical assays.

All of our equipment is fully tested to perform at factory specifications before being shipped.

The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader includes:
  • > Windows 10 Data Station with Pre-Loaded Gen5 PC Software
  • > 460 nm, 528 nm, 590 nm, and 645 nm Emission Filters
  • > 340 nm, 460 nm, 485 nm, and 540 nm Excitation Filters
  • > ABS, FLD, LUM, and Self Test Calibration Report
  • > Interface Cable
  • > Operator's Manual
  • > Full 180 Day Part and Labor Warranty

BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader Specifications

Absorbance Specifications

Light Source

Xenon flash lamp

Wavelength Selection


Wavelength Range

230 to 999 nm, in 1 nm increments


2 nm (230 to 285 nm), 4 nm (> 285 nm)

Dynamic Range

0 to 4.0 OD

Photometric Resolution

0.0001 OD

Pathlength Correction


Photometric Accuracy

< 1% at 2.0 OD typical

< 3% at 3.0 OD typical

Photometric Repeatability

< 0.5% at 2.0 OD typical

Fluorescence Intensity Specifications

Light Source

Tungsten halogen lamp

Wavelength Selection

Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top)

Wavelength Range

Monochromators: 250 to 850 nm

Filters: 200 to 700 nm (850 nm option)

Dynamic Range

Monochromators: 5 decades

Filters/Mirrors: > 6 decades


Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate)

Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)

Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)

Luminescence Specifications

Wavelength Range

300 to 700 nm

Dynamic Range

< 6 decades


10 amol ATP typical (flash)

Fluorescence Polarization Specifications

Light Source

Tungsten halogen

Wavelength Selection

Deep blocking filters/dichroic mirrors (top)

Wavelength Range

400 to 700 nm (320 to 850 nm option)


3 mP at 1 nm fluorescein typical

Time-Resolved Fluorescence Specifications

Light Source

High energy xenon flash lamp

Wavelength Range

200 to 700 nm (850 nm option)


Europium 60 fM typical with filters (6 amol/well in 384-well plate)

Reagent Dispenser Specifications


2 syringe pumps

Dispense Volume

5 to 1000 ul in 1 ul increments

Minimum Prime Volume

1.1 mL, 100 ul with back flush

General Specifications

Microplate Formats

Monochromator System: 1 to 384-wells

Filter System: 1 to 1536-wells

PCR plates, and Take3 Micro-Volume Plates

Read Methods

End point, kinetic, spectral scanning, well area scanning

Detection Methods

FL, TRF, FP, Lum., UV-Vis Abs., AlphaScreen/AlphaLISA

Plate Shaking


Temperature Control

Ambient +4°C to 65°C, ± 0.5°C

Dimensions (W x D x H)

17 x 20.9 x 15 in. (43.5 x 53.1 x 38.1 cm)


78 lb. (35 kg.)

Electrical Requirements

100-240V, 50/60Hz

Computer Specifications




Intel Core i5-7200U CPU


16.0 GB

Hard Drive

240GB Internal SSD

Operating System

Windows 10 Professional, 64-bit


Why Buy From Marshall Scientific?

Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.

All Lab Equipment is Fully Recertified and Tested

Most Pieces Ship within 1 to 3 Business Days

All Products are Fully Warrantied

Trained Customer and Technical Support

Industry Leading Overall Customer Satisfaction

Bausch + Lomb
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Mass General Hospital
My recently purchased microplate reader from Marshall Scientific has been a much needed addition to my lab. The reader is easy to use and provides accurate and reliable results. I have used it for various experiments including ELISA, cell viability assays, and protein quantification. The reader has greatly improved the efficiency and accuracy of my experiments. Thanks again!
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