Eppendorf BioSpectrometer Basic Model #6135

The Eppendorf BioSpectrometer Basic Model #6135 is a small, very compact spectrophotometer that combines easy handling with highly reproducible analysis. It can measure and record UV/Vis spectral ranges as well as measure individual wavelengths from 200 nm to 830 nm. Its freely selectable wavelengths provide maximum flexibility for all current and future applications. In addition to preprogrammed methods for standard measurements, users can create and store their own methods. Optimized menu navigation guides through the individual methods in a step-by-step process. All required entries are visible, no essential data is omitted. A help box explains the operational sequences and is available in 5 different languages.

The Eppendorf BioSpectrometer Basic Model #6135 facilitates absorbance measurements at one or multiple wavelengths, offering the capability to record wavelength scans ranging from 200 nm to 830 nm with 1 nm increments. It automatically evaluates and stores measurement data while presenting results clearly, including spectral graphs for sample purity assessment with automatic ratio calculations. The device offers preprogrammed applications for swift initiation or the flexibility to create custom applications, enabling evaluation through factors, standards, or standard series, all with freely programmable parameters. Additionally, it supports a two-wavelength method with subtraction or division for analysis. Data can be conveniently transferred via USB, Ethernet, or email, with direct result printing. The software incorporates guided processes to minimize errors and includes an integrated self-test and calibration history tracking for enhanced reliability.

Applications include: Nucleic acid quantification, Direct protein quantification (UV 280 nm), Microvolume measurement via µCuvette G1.0 for highly concentrated samples, Bacteria growth measurement (OD 600), Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry, Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins), Free selectable wavelengths, and more.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Eppendorf BioSpectrometer Basic Model #6135 Specifications

Measuring Principle	Single beam absorption spectrophotometer with reference beam
Light Source	Xenon flash lamp
Monochromator	Holographic aberration-corrected concave grating
Beam Receiver	CMOS photodiode array
Wavelength Range	200 nm to 830 nm
Wavelength Selection	Method-dependent, freely selectable
Spectral Bandwidth	≤ 4 nm
Smallest Step Size	1 nm
Systematic Wavelength Error	± 1 nm
Random Wavelength Error	≤ 0.5 nm
Photometric Measuring Range	0 A to 3.0 A at 260 nm
Reading Accuracy	ΔA = 0.001
Systematic Photometric Error	± 1 % at A = 1
Random Photometric Error	≤ 0.002 at A = 0 ≤ 0.005 (0.5 %) at A = 1
Stray Light Component	< 0.05 %
Cuvette Material	For measurement in UV Range: Quartz glass or UV transparent plastic For measurement in visible range: Glass or plastic
Cuvette Shaft	12.5 mm x 12.5 mm, untempered
Overall Cuvette Height	Minimum of 36 mm
Height of Light Beam in Cuvette	8.5 mm
Result Output	Absorbance, transmission, concentration, scan (aborsbance wavelength spectrum)
Dimensions (W x D x H)	11.6 x 15.7 x 5.9 in. (29.5 x 40 x 15 cm)
Weight	11.1 lb. (5 kg)
Electrical Requirements	100-240V, 50/60Hz, 0.5A, 25W