Setable Flow Range
0.001 ‚ 10 ml/min, in 0.001 ml/min increments
Flow Range
0.2 ‚ 10.0 ml/min
Flow Precision
< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min
Pressure
Operating range 0 ‚ 40 MPa (0 ‚ 400 bar, 0 ‚ 5880 psi) up to 5 ml/min
Operating range 0 ‚ 20 MPa (0 ‚ 200 bar, 0 ‚ 2950 psi) up to 10 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)
Recommended pH Range
1.0 ‚ 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Gradient Formation
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay volume 800 ‚ 1100 µl, dependent on back pressure
Composition Range
0 ‚ 95% or 5 ‚ 100%, user selectable
Composition Precision
< 0.2% SD, at 0.2 and 1 ml/min
Dimensions (H x W x D)
5.5 x 13.5 x 17 in
Electrical Requirements
100-120 or 22-240 VAC, 50/60 Hz, 220 VA
Temperature Range
Range 10° below ambient to 80° C
Temperature Stability
± 0.15°C
Temperature Accuracy
± 0.8°C
Column Capacity
Three 30cm columns
Warm-up/Cool Down
5 mins from ambient to 40°C
Analysis Run Time
30 to 45 min
Number of Samples
Up to 12 samples/chips
Sample Volume
1 µl
Sizing Range
50 to 7000 bp
Ambient Operating Temperature
5° to 40°C (41° to 104°F)
100-240VAC, 50-60Hz
Power Consumption
60 VA
Dimensions (W x D x H)
162 x 412 x 290 mm (6.4 x 16.2 x 11.4 in)
Safety Standards: IEC, CSA, UL
Installation Category II, Pollution Degree 2
Number of Channels
4
Internal Volume per Channel
Typically 1 ml per channel
Materials in Contact with Solvent
PTFE, FEP, PEEK
pH Range
1 to 14
RS-232 Output
For diagnostic purposes
Max Flow Rate
10 ml/min per channel
Reduced performance above 5 ml/min
Internal Volume
1 ml/channel
PTE, PEEK
1 ‚ 14
Analog Output (AUX)
For pressure monitoring, range 0 ‚ 3 V
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
100-120 or 220-240 VAC, 50/60 Hz
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
30 W
Line Voltage
100-120 220-240 VAC
Line Frequency
50 r 60 Hz
Weight
7kg (15.4 lbs)
13.5 x 17 x 3.1 in
GLP Features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications
Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features
Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range
0.1 ‚ 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification
required)
Replicate Injections
1 ‚ 99 from one vial
Precision
Typically < 0.5 % RSD of peak areas from 5 ‚ 100 µl
Typically < 1 % RSD of peak areas from 1 ‚ 5 µl
Minimum Sample Volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Carryover
Typically < 0.1 %, < 0.05 % with external needle cleaning
Sample Viscosity Range
0.2 ‚ 50 cp
Replicate Injections Per Vial
1 ‚ 99
Sample Capacity
100 ƒ” 2-ml vials in 1 tray
40 ƒ” 2-ml vials in ½ tray
15 ƒ” 6-ml vials in ½ tray (Agilent vials only)
Injection Cycle Time
Typically 50 s depending on draw speed and injection volume
Hydraulic system
Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Setable flow range
Setpoints 0.001 ‚ 5 ml/min, in 0.001 ml/min increments
Flow range
0.1 ‚ 5.0 ml/min
Flow precision
£ 0.07% RSD, or £ 0.02 min SD whatever is greater, based on retention time at constant room temperature
Flow accuracy
± 1% or 10 µl/min whatever is greater
Operating range 0 400 bar (0 ‚ 5880 psi) up to 5 ml/min
Pressure pulsation
< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility compensation
User-selectable, based on mobile phase compressibility
Recommended pH range
1.0 ‚ 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation
High-pressure binary mixing, delay volume 180 ‚ 480 µl without mixer, 600 ‚ 900 µl with mixer, dependent on back pressure
Composition range
1 ‚ 99 % or 5 µl/min per channel, whatever is greater
Composition precision
£ 0.5% absolute
Composition accuracy
± 0.15% RSD, at 1 ml/min