Flow Channel Diameter
1.2 millimeters
Maximum Pressure
2 MPa, 20 bar, 290 psi
Valve type
Motorized rotary 8-way valve
Flow rate
Up to 100 milliliters per minute
Max Flow Rate
100 ml/min
Max Pressure
25 MPa (250 bar, 3600 psi)
Back Pressure
< 40 kPa at 100 ml/min with water
Leakage
<0.1 ul/min at 25 MPa
pH Stability Range
1-13, 1-14 (<1 day exposure)
Viscosity
Max 5 cP
Switch Time
< 260 ms between two adjacent positions
Operating Life Time
> 50,000 cycles, two adjacent positions
1.2 mm
<50 kPa at 100 ml/min with water
<260 ms between two adjacent positions
0.001 ‚ 100 ml/min
12 ml chamber: 10 MPa at 0.1 ‚ 100 ml/min (100 bar, 1450 psi)0.6, 2, and 5 ml chambers: 25 MPa at 0.001 ‚ 100 ml/min (250 bar, 3625 psi)90 µl and 0.2 ml chambers: 35 MPa at 0.001 ‚ 5 ml/min (350 bar, 5075 psi)
pH Range
1 ‚ 13, 1 ‚ 14 (< 1 day exposure)
Internal Volume
6 interchangeable mixing chambers: 90 µl, 0.2, 0.6, 2, 5, and 12 ml
1 static chamber and 1 dynamic chamber
Operating Temperature Range
4°C to 40°C
Operating Humidity Range
< 95% non-condensing
Max Power Consumption
260 VA (210 W)
BTU
717
Temperature Range
4° to 40°C
Ambient Temperature
Dimensions
140 x 345 x 435 mm
Line Voltage
100-120 or 220-240 VAC
Line Frequency
50/60 Hz
Range 10° below ambient to 80° C
Temperature Stability
± 0.15°C
Temperature Accuracy
± 0.8°C
Column Capacity
Three 30cm columns
Warm-up/Cool Down
5 mins from ambient to 40°C
Operating Pressure Max.
50 bar (5 MPa) (725 psi)
1 µS/cm-999.9 mS/cm
pH Stability Long Term
2-12
260 x 370 x 100 mm
Accuracy
The greater of ± 2% of full scale or ± 10 µS/cm in the range 1 µS/cm-300 mS/cm ± 0.1 pH unit, temperature compensated Max. 0.1 pH units within 0‚10 ml/min (pH measurement)
Baseline noise
± 0.5% of full scale calibrated range
Drift
Max. 0.02 pH/h (measured at pH 4.0) ±0.5 °C per 10 h 0.1 pH unit deviation/10 h
Cell Constant
50 ± 20 cm-1
Response Time
Max. 10 s (0‚95% of step) (pH measurements) Max. 3 s (0‚95% of step) (Conductivity measurement)
100-120/220-240 VAC
10 ml/min per channel
Reduced performance above 5 ml/min
Number of Channels
4
1 ml/channel
Materials in Contact with Solvent
PTE, PEEK
1 ‚ 14
Analog Output (AUX)
For pressure monitoring, range 0 ‚ 3 V
Dimensions (W x D x H)
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
Electrical Requirements
100-120 or 220-240 VAC, 50/60 Hz
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
Ambient Operating Temperature
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
Power Consumption
30 W
100-120 220-240 VAC
50 r 60 Hz
Weight
7kg (15.4 lbs)
13.5 x 17 x 3.1 in
3-Way Valves
3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 200 mL/min with standard or large bore needle
Communication Interface
RS-232 or GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices
Data Acquisition
An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC
Detector Input
±100 mV full scale
Drop Counting
Up to 9,999 drops per fraction
maximum rate: 20 drops/sec
Event Marker
100 ms pulse
Front Panel
Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0‚9 numeric hard keys; four soft keys
Maximum Collection Volume/Tube
32 mL (18 x 180 mm tubes) or 25 mL (18 x 150 mm)
Maximum Fractions
Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)
Multiple-Column Collection
Simultaneous collection from up to 18 columns with installation of optional multiple column adaptor(s)
Multiple-Cycle Operation
Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes 3-port, PTFE, 114 mL internal volume, 6 µL dead volume from the common port to the normally closed port, up to 200 mL/min with large bore needle
Number of Racks
Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)
Operating Modes
Drop, Manual, Peak + Drop, Peak + Time, Time
Peak Detection
Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value
Peak parameters: peak height or level, in mV and peak width at half-height, in minutes
Minimum peak height for fractionation: 0.1 mV full scale
Detector-collector delay: 0.01 minute increments
Power Requirements
120V, 50/60Hz, 0.5A
Programmable Time Units
From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution
Time-Based Programming
Up to 10 collection windows and 10 drain steps in any mode
Tube Change Time
100‚720 ms, center to center, depending on rack type
47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)
4ƒš° to 40ƒš°C
Temperature accuracy at ambient
temperatures < 25 ƒš°C and humidity < 50%
- 1ƒš°C to + 4 ƒš°C at a setpoint of 4 ƒš°C
temperatures > 25 ƒš°C and/or humidity > 50%
- 1ƒš°C to + 5 ƒš°C at a setpoint of 4 ƒš°C
Internal Volume per Channel
Typically 1 ml per channel
PTFE, FEP, PEEK
RS-232 Output
For diagnostic purposes
Typically 12 ml per channel
PTFE, PEEK
Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 50%)
3°C to 8°C at a setpoint of 4°C
Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 60%)
3°C to 9°C at a setpoint of 4°C
46 lbs. (20.7 kg)
Flow Rate
Up to 50 ml
1 MPa
Operating Temperature
Hydraulic System
Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve
Setable Flow Range
0.001 ‚ 10 ml/min, in 0.001 ml/min increments
Flow Range
0.2 ‚ 10.0 ml/min
Flow Precision
<0.07% RSD, or <0.02 min SD whatever is greater, based on retention time at constant room temperature
Flow Accuracy
±1% or 10µl/min whatever is greater
Pressure
Operating range 0 ‚ 40 MPa (0 ‚ 400 bar, 0 ‚ 5880 psi) up to 5 ml/min Operating range 0 ‚ 20 MPa (0 ‚ 200 bar, 0 ‚ 2950 psi) up to 10 ml/min
Pressure Pulsation
< 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 10 bar (147 psi)
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Recommended PH Range
1.0 ‚ 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Control and Data Evaluation
Agilent ChemStation for LC
Analog Output
For pressure monitoring, 2 mV/bar, one output
Communications
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
1 to 14
95 fractions in 10-18 mm tubes
< 100 ml/min
1 ‚ 13
pH Stability Short Term
Program Memory
99 programs
95 x 10-18 mm (Standard)
175 x 12 mm and 40 x 30 mm (Optional)
Time Mode
0.01 ‚ 9.999 min
Volume Mode
0.1 ‚ 999 ml
Voltage
100 ‚ 240 V
20 bar (2 MPa, 290 psi)
Dimensions (H x W x D)
158 x 148 x 99 mm
± 0.75 ƒ” 10-5AU at 254 nm
3 ƒ” 10-4AU/hr at 254 nm
Standard: 14-µl volume, 10-mm
cell path length and 40 bar
(588 psi) pressure maximum
High pressure: 14-µl volume,
10-mm cell path length and
400 bar (5880 psi) pressure
maximum
Micro: 1-µl volume, 5-mm cell
path length and 40 bar (588 psi)
pressure maximum
Semimicro: 5-µl volume, 6-mm
Two dual piston in series, with proprietary servo-controlled variable stroke drive, floating piston, active inlet valve, solvent selection valve and electronic flow control for flow rates up to 100 µl/min
Settable Column Flow Range
0.01 ‚ 20 µl/min
0.01 ‚ 100 µl/min (with the extended flow range kit)
0.001 ‚ 2.5 µl/min (with the electronic flow control bypassed)
Recommended Column Flow Range
1 ‚ 20 µl/min
10 ‚ 100 µl/min (with extended flow range kit)
0.1 ‚ 2.5 ml/min (with the electronic flow sensor bypassed)
Column Flow Precision
< 0.7 % RSD or 0.03 % SD (typically 0.4 % RSD or 0.02 % SD), at 10 µl/min and 50 µl/min column flow (based on RT, default setting)
Optimum Composition Range
1 to 99% or 5 µl/min per channel (primary flow), whatever is greater
Composition Precision
< 0.2 % SD, at 10 µl/min (20 µl flow sensor), 50 µl/min (100 µl flow sensor) and 1 ml/min (normal mode) default setting
Delay Volume
Typically 3 µl from the electronic flow control to the pump outlet for flow rates up to 20 µl/min.
Typically 12 µl from the electronic flow control to the pump outlet for flow rates up to 100 µl.
for flow rates up to 100 µl/min and electronic flow control active: primary flow path 180 - 480 µl without mixer, 600 - 900 µl with mixer (system pressure dependant)
Typically 180 to 480 µl (system pressure dependent) without mixer for flow rates up to 2.5 ml/min. (Mixer delay volume 420 µl)
Pressure Range
20 to 400 bar (5880 psi) system pressure
Recommended pH Range
1.0 ‚ 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries.
Safety and Maintenance
Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling,
leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
GLP Features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors.
Housing
All materials recyclable.
14.2 kg (31.3 lbs)
200 x 345 x 435 mm (8 x 13.5 x 17 in)
Line frequency
100 ‚ 120 or 220 ‚ 240 VAC, ± 10 %
50 or 60 Hz, ± 5 %
Power consumption (apparent power)
Power consumption (active power)
300 VA max.
200 W max.
Ambient operating temperature
Ambient non-operating temperature
4 ‚ 55 °C (41 ‚ 131 °F)
40 to 70 °C (-4 to 158 °F)
Humidity
< 95 %, at 25 ‚ 40 °C (77 ‚ 104 °F) non-condensing
Tray
Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)
Settable injection volume
0.01 to 8 µl with small loop capillary
0.01 to 40 µl with extended loop capillary
Precision
Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl
Minimum sample volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials
Carryover
Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume
Sample viscosity range
0.2 - 5 cp
Recommended pH-range
1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries
Material in contact with solvent
Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel
GLP features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
All materials are recyclable
0.1 to 99999.99 ml
Height
480 mm
pH Stability range
1 to 13, 1 to 14 (<1 day exposure)
