The Waters 600 The Waters 600 controller automatically controls the eluent gradient, the flow rate, column heating, external events, and sparging. The Waters 600 Controller can be configured as a PowerLine system controller or as a Gradient controller. The PowerLine configuration is the default configuration that includes programming methods, external device control, and an RS-232 interface. For use within Waters 600 HPLC System.
All of our equipment is fully tested to perform at factory specifications before being shipped.Included with the Waters 600 Controller:
Max Pressure
2 MPa (20 bar, 290 psi)
Flow Channel Diameter
1.2 mm
Flow Rate
Up to 100 ml/mm
GE Product #
18-1108-42
Max Flow Rate
100 ml/min
25 MPa (250 bar, 3600 psi)
Back Pressure
<50 kPa at 100 ml/min with water
Leakage
<0.1 ul/min at 25 MPa
pH Stability Range
1-13, 1-14 (<1 day exposure)
Viscosity
Max 5 cP
Switch Time
<260 ms between two adjacent positions
Operating Lifetime
>50,000 cycles, two adjacent positions
Environment
4° to 40°C
20-95% relative humidity
84-100 kPa (840-1060 mbar) atmospheric pressure
Fraction Size
0.05 – 100 ml (0.001 ml increments)
Time Mode
0.01 – 9.999 min
Volume Mode
0.01 – 9.999 ml
Rack Size
95 places for tubes ranging between 10 -18mm
100ml/min
0.001 – 100 ml/min
12 ml chamber: 10 MPa at 0.1 – 100 ml/min (100 bar, 1450 psi)
0.6, 2, and 5 ml chambers: 25 MPa at 0.001 – 100 ml/min (250 bar, 3625 psi)
90 µl and 0.2 ml chambers: 35 MPa at 0.001 – 5 ml/min (350 bar, 5075 psi)
pH Range
1 – 13, 1 – 14 (< 1 day exposure)
Internal Volume
6 interchangeable mixing chambers: 90 µl, 0.2, 0.6, 2, 5, and 12 ml
Mixing Principle
1 static chamber and 1 dynamic chamber
Power Requirement
12 – 40 V DC from the pump
Power Consumption
1 W
3-Way Valves
3-port, PTFE, 67 µL internal volume, 3.5 µL dead volume from common port to the normally closed port, up to 20 mL/min with standard or large bore needle
Communication Interface
GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices
Data Acquisition
An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC
Detector Input
±100 mV full scale
Drop Counting
Up to 9,999 drops per fraction
maximum rate: 20 drops/sec
Event Marker
100 ms pulse
Front Panel
Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0–9 numeric hard keys; four soft keys
Maximum Collection Volume/Tube
20 mL (28 x 60 mm scintillation vials with optional Code 24 rack)
Maximum Fractions
128 (12 x 75 mm tubes with optional Code 14 rack)
Multiple-Column Collection
Simultaneous collection from up to 8
Multiple-Cycle Operation
Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes
Number of Racks
Holds one rack
Operating Modes
Drop, Manual, Peak + Drop, Peak + Time, Time
Peak Detection
Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value
Peak parameters: peak height or level, in mV and peak width at half-height, in minutes
Minimum peak height for fractionation: 0.1 mV full scale
Detector-collector delay: 0.01 minute increments
Power Requirements
120V, 50/60Hz, 0.5A
Programmable Time Units
From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution
Time-Based Programming
Up to 10 collection windows and 10 drain steps in any mode
Tube Change Time
100—250 ms, center to center, depending on rack type
Dimensions (W x D x H)
32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in)
3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 200 mL/min with standard or large bore needle
RS-232 or GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices
32 mL (18 x 180 mm tubes) or 25 mL (18 x 150 mm)
Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)
Simultaneous collection from up to 18 columns with installation of optional multiple column adaptor(s)
Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes 3-port, PTFE, 114 mL internal volume, 6 µL dead volume from the common port to the normally closed port, up to 200 mL/min with large bore needle
Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)
100—720 ms, center to center, depending on rack type
47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)
Operating Temperature Range
4°C to 40°C
Operating Humidity Range
< 95% non-condensing
Max Power Consumption
260 VA (210 W)
BTU
717
Temperature Range
Ambient Temperature
Dimensions
140 x 345 x 435 mm
Line Voltage
100-120 or 220-240 VAC
Line Frequency
50/60 Hz
10° below ambient to 80° C
Temperature Stability
± 0.15°C
Temperature Accuracy
± 0.8°C
Column Capacity
Three 30cm columns
Warm-up/Cool Down
5 mins from ambient to 40°C
Dead Volume
3 ul left heat exchanger6 ul right heat exchanger
Dimensions (H x W x D)
5.5 x 16 x 17 in (14 x 41 x 43.5 cm)
Up to 50 ml
Maximum Pressure
1 MPa
Operating Temperature
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
Ambient Operating Temperature
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
30 W
100-120 220-240 VAC
50 r 60 Hz
Weight
7kg (15.4 lbs)
13.5 x 17 x 3.1 in
Wavelength Range
190 to 800 nm
Wavelength Accuracy
±1 nm
Linearity Range
5% over -0.1 to 2.0 AU
Spectral Resolution
1.2 nm per photodiode
Baseline Noise
5 x 10 -5 AU peak-to-peak
Drift
1 x 10 -3 AU/hour at 254 nm (after warmup)
4 to 40°C (39° to 104°F)
Operating Humidity
20 to 80% non-condensing
Flow Cells
Standard Pathlength: 10 mm
Semi-Preparative Pathlength: 3 mm
Microbore Pathlength: 6 mm
Inert Pathlength: 10 mm
Range 10° below ambient to 80° C
10 ml/min per channel
Reduced performance above 5 ml/min
Number of Channels
4
1 ml/channel
Materials in Contact with Solvent
PTE, PEEK
1 – 14
Analog Output (AUX)
For pressure monitoring, range 0 – 3 V
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
Electrical Requirements
100-120 or 220-240 VAC, 50/60 Hz
Temperature accuracy at ambient
temperatures < 25 °C and humidity < 50%
- 1°C to + 4 °C at a setpoint of 4 °C
temperatures > 25 °C and/or humidity > 50%
- 1°C to + 5 °C at a setpoint of 4 °C
190 to 700 nm
Bandwidth
5 nm
± 1 nm
Wavelength Repeatability
± .10 nm
Noise, single wavelength
< ± 0.35 x 10-5 AU, dry cell, 254 nm, 1.0 sec. time constant (TC)
Noise, dual wavelength
< ± 2.5 x 10-5 AU, dry cell, 254 nm / 280 nm, 2.0 sec. time constant (TC)
Linearity
5% at 2.5 AU, dry cell, 254 nm / 280 nm, 2.0 sec. time constant (TC)
1.0 x 10^4 AU/Hour/degrees Celcius at 254 nm (after 1 hour warmup)
Sensitivity setting range
0.0001 to 4.0000 AUFS
Capacity
95 fractions in 10-18 mm tubes
Depth
400 mm
< 100 ml/min
Height
250 mm
pH Stability Long Term
1 – 13
pH Stability Short Term
Program Memory
99 programs
Rack Designation
95 x 10-18 mm (Standard)
175 x 12 mm and 40 x 30 mm (Optional)
Relative Humidity
20 – 95% (none condensing)
0.1 – 999 ml
Voltage
100 – 240 V
20 bar (2 MPa, 290 psi)
158 x 148 x 99 mm
Typically 12 ml per channel
PTFE, PEEK
Internal Volume per Channel
Typically 1 ml per channel
PTFE, FEP, PEEK
RS-232 Output
For diagnostic purposes
0.1 to 99999.99 ml
480 mm
pH Stability range
1 to 13, 1 to 14 (<1 day exposure)
± 0.75 × 10-5AU at 254 nm
3 × 10-4AU/hr at 254 nm
Standard: 14-µl volume, 10-mm
cell path length and 40 bar
(588 psi) pressure maximum
High pressure: 14-µl volume,
10-mm cell path length and
400 bar (5880 psi) pressure
maximum
Micro: 1-µl volume, 5-mm cell
path length and 40 bar (588 psi)
pressure maximum
Semimicro: 5-µl volume, 6-mm
5% at 2.0 AU, propylparaben, at 256 nm
1.2 nm
±1.5 x 10^-5 AU peak-to-peak, dry, at 254 nm
1 x 10^-3 AU/hour at 254 nm (after warmup)
∆T ≤ 1°C per hour
11.5 x 24 x 8.5 in (29 x 61 x 22 cm)
Low trigger < 1.8 V
High trigger > 3.0 V
Protected to ±30 V
Minimum pulse width 20 msec
Maximum current 5 mA
Detection Type
1024-element photodiode array
Light Source
Deuterium and tungsten lamps
190 – 950 nm
Short Term Noise (ASTM) Single and Multi-Wavelength
± 1 × 10-5AU at 254 and 750 nm
2 × 10-3AU/hr at 254 nm
Linear Absorbance Range
> 2 AU (upper limit)
Wavelength Bunching
1 – 400 nm
Slit Width
1, 2, 4, 8, 16 nm
Diode Width
< 1 nm
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum
500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and Data Evaluation
Agilent ChemStation for LC
Analog Outputs
Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
Communications
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and Maintenance
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
GLP Features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter
Housing
All materials recyclable.
Setable Flow Range
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range
0.1 to 5 ml/min
Flow Precision
≤ 0.07% RSD or ≤ 0.02 min SD
Flow Accuracy
± 1% or 10 ul/min
Pressure
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Hydraulic System
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Recommended pH Range
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range
1 to 99% or 5 ul/min per channel
Composition Precision
≤ 0.5% absolute
Composition Accuracy
≤ 0.15% RSD at 1 ml/min
Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximumHigh pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximumMicro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximumSemimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
