± 0.75 × 10-5AU at 254 nm

3 × 10-4AU/hr at 254 nm

Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum
High pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximum
Micro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum
Semimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum

Type

Specification

Detection type

1024-element photodiode array

Light Source

Deuterium and tungsten lamps

Wavelength range

190 – 950 nm

Short term noise (ASTM) single and multi-Wavelength

± 1 × 10-5 AU at 254 and 750 nm

Drift

2 × 10-3 AU/hr at 254 nm

Linear absorbance

range

> 2 AU (upper limit)

Wavelength Accuracy

± 1 nm

Wavelength bunching

1 – 400 nm

Slit width

1, 2, 4, 8, 16 nm

Diode Width

< 1 nm

Flow Cells

Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum

Control and data evaluation

Agilent ChemStation for LC

Analog outputs

Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs

Communications

Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional

Safety and maintainence

Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas

GLP features

Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.

Housing

All materials recyclable

± 0.75 × 10-5AU at 254 nm

3 × 10-4AU/hr at 254 nm

Standard: 14-µl volume, 10-mm

cell path length and 40 bar

(588 psi) pressure maximum

High pressure: 14-µl volume,

10-mm cell path length and

400 bar (5880 psi) pressure

maximum

Micro: 1-µl volume, 5-mm cell

path length and 40 bar (588 psi)

pressure maximum

Semimicro: 5-µl volume, 6-mm

cell path length and 40 bar

(588 psi) pressure maximum

Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W

Pulse frequency

296 Hz for single signal mode 74 Hz for spectral mode

Excitation Monochromator

Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm

Reference System:

in-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Spectrum acquisition:

Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All

Wavelength characteristic

Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz