± 0.75 × 10-5AU at 254 nm
3 × 10-4AU/hr at 254 nm
Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximumHigh pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximumMicro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximumSemimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
Type
Specification
Detection type
1024-element photodiode array
Light Source
Deuterium and tungsten lamps
Wavelength range
190 – 950 nm
Short term noise (ASTM) single and multi-Wavelength
± 1 × 10-5 AU at 254 and 750 nm
Drift
2 × 10-3 AU/hr at 254 nm
Linear absorbance
range
> 2 AU (upper limit)
Wavelength Accuracy
± 1 nm
Wavelength bunching
1 – 400 nm
Slit width
1, 2, 4, 8, 16 nm
Diode Width
< 1 nm
Flow Cells
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and data evaluation
Agilent ChemStation for LC
Analog outputs
Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
Communications
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and maintainence
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas
GLP features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
Housing
All materials recyclable
Standard: 14-µl volume, 10-mm
cell path length and 40 bar
(588 psi) pressure maximum
High pressure: 14-µl volume,
10-mm cell path length and
400 bar (5880 psi) pressure
maximum
Micro: 1-µl volume, 5-mm cell
path length and 40 bar (588 psi)
pressure maximum
Semimicro: 5-µl volume, 6-mm
Detection Type
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
Pulse frequency
296 Hz for single signal mode 74 Hz for spectral mode
Excitation Monochromator
Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm
Reference System:
in-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition:
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
