Trigger Modes
Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
Operating Modes
Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
Test Tube/Plate Sizes
Minimum 48 mm to 75 mm maximum
Maximum Tube Volume
ca. 45 ml
Maximum Flow Rate
100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
Delay Volumes [μl]
Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
Diverter valve: ~15
Diverter valve to needle: ~110
Needle: -~5
Delay Calibration Sensor
Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
Diverter Valve
3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
Maximum Capacity
3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
Interfaces
Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
Safety Features
Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors
Delay Volume (in ul)
Fraction collector inlet to diverter valve: ~50 (typical, depends on length of the tubing)
Diverter valve to needle: ~10
Needle: ~4
~20 mL with 30 x 48 mm (OD x L) tube
~30 mL with 30 x 75 mm (OD x L) tube
Time slices
Peak (threshold, up- / downslope)
Timetable (combination of time intervals and peak)
Manual trigger (supported only with Agilent Instant Pilot G4208A)
48 mm with long needle assembly G1367-87200
75 mm with short needle assembly G1364-87202
Fraction collector inlet to diverter valve: ~500 (typical, depends on length of the tubing)
Needle: ~5
Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position
Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
~20 mL with 48 mm tubes
~30 mL with 75 mm tubes
Continuous flow: optional, available only when using deep well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
Needle into location: Needle pushes into the vessel as deep as specified, for the use with capped vials and test tubes and well plates with closing mats
Droplet setup mode: The tip of the fraction collector needle will initially move down to the bottom of the well. Then it will slowly move upwards while the fraction is collected. The droplet setup mode enables the fraction collector to collect small fractions without bubbles
4 x well-plates full tray (MTP)*
2 x well-plates std. tray + 10 funnels with external containers* (+ 1 half tray)
2 × well-plates std. tray (MTP) + 10 × 2 ml vials* (+ 1 half tray)
100 x 2 ml in std. tray (+ 1 half tray)*
3 x 40 x 2 ml in half tray*
3 x 40 funnels in half tray
3 x 15 x 6 ml in half tray*
Full tray with 40 test tubes (30 mm OD, max. height 48 mm, ~20 ml vol.)
Full tray with 60 test tubes (25 mm OD, max. height 48 mm
Full tray with 126 test tubes (16 mm OD, max. height 48 mm)
Full tray with 215 test tubes (12 mm OD, max. height 48 mm)
