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Agilent 1200 HPLC G1312B SL Binary Pump Agilent 1200 HPLC G1312B SL Binary Pump

Hydraulic system Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Setable flow range 0.001 to 10 mL/min, in 0.001 mL/min increments
Flow range 0.05 to 10.0 mL/min
Flow precision < 0.07% RSD or < 0.02 min SD, whatever is greater based on retention time at constant ambient temperature
Flow accuracy ± 1% or 10 µl/min whatever is greater
Pressure Operating range 0 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure pulsation < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility compensation User-selectable, based on mobile phase compressibility
Recommended pH range 1.0 to 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation High-pressure binary mixing, delay volume 180 ‚ 480 µl without mixer, 600 to 900 µl with mixer, dependent on back pressure
Composition range 1 to 99 % or 5 µl/min per channel, whatever is greater
Composition precision < 0.5% absolute
Composition accuracy ± 0.15% RSD, at 1 ml/min

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Agilent G4208A Series Pilot Controller Agilent G4208A Series Pilot Controller

Agilent 1200 G4208A Series Pilot Controller Specifications

Type

Specification

Weight

0.8 kg

Dimensions (width x depth x height)

130 x 225 x 35 mm (5.1 x 8.9 x 1.4 inches)

Line voltage

22 VDC, ± 10 %

Power consumption

6 W / 20.5 BTU/hour

Ambient operating temperature

0 to 55C (32 to 131F)

Ambient non-operating temperature

-40 to 70C (-40 to 158F)

Humidty

< 95%, at 25 ‚ 40C (77 to 104F)

Operating Altitude

Up to 2000 m (6562 ft)

Non-operating altitude

Up to 4600 m (15092 ft)

Safety standards: IEC, CSA, UL, EN

Installation category II, pollution degree 2. For indoor use only. Research Use Only. Not for use in Diagnostic Procedures.

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Agilent 1200 HPLC G1311A Quat Pump Agilent 1200 HPLC G1311A Quat Pump

Agilent 1200 HPLC G1311A Quat Pump Specifications

Setable Flow Range

0.001 to 10 ml/min, in 0.001 ml/min increments

Flow Range

0.2 to 10.0 ml/min

Flow Precision

< 0.07% RSD, or < 0.02 min SD whatever is greater, based n retention time at constant room temperature

Flow Accuracy

± 1% or 10 µl/min whatever is greater

Pressure

Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min

Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min

Pressure Pulsation

< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)

Recommended pH Range

1.0 to 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel

Gradient Formation

Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve

Delay volume 800 – 1100 µl, dependent on back pressure

Composition Range

0 to 95% or 5 to 100%, user selectable

Composition Precision

< 0.2% SD, at 0.2 and 1 ml/min

Dimensions (H x W x D)

5.5 x 13.5 x 17 in

Electrical Requirements

100-120 or 22-240 VAC, 50/60 Hz, 220 VA

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Agilent 1200 HPLC G1329A Autosampler Agilent 1200 HPLC G1329A Autosampler

Agilent 1200 HPLC G1329A Autosampler Specifications

GLP Features Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range 0.1 to 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
Replicate Injections 1 to 99 from one vial
Precision Typically < 0.5 % RSD of peak areas from 5 to 100 µl Typically < 1 % RSD 1 to 5 µl variable volume
Minimum Sample Volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Carryover Typically < 0.1 %, < 0.05 % with external needle cleaning
Sample Viscosity Range 0.2 to 50 cp
Replicate Injections Per Vial 1 to 99
Sample Capacity 100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray (Agilent vials only)
Injection Cycle Time Typically 50 s depending on draw speed and injection volume

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Agilent 1200 HPLC G1367B High Performance Autosampler Agilent 1200 HPLC G1367B High Performance Autosampler

Agilent 1200 HPLC G1367B High Performance Autosampler Specifications

GLP Features Early maintainece feedback (EMF), electronic records of maintainence and errors
Communications Controller-area network (CAN). RS232C, APG remote standard, optional four external contact closures and BCD vial number output
Safety Features Leak detection and safe leak handling, low voltages in maintence areas, error detection and display
Injection Range 0.1 to 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
Precision Typically < 0.25 % RSD from 5 ‚ 100 µl, Typically < 1 % RSD from 1 - 5 µl
Pressure Range 400 bar (5800 psi)
Sample Viscosity Range 0.2 to 5 cp
Sample Capacity 2 × well-plates (MTP) + 10 × 2 ml vials 108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate
Injection Cycle Time Typically < 30 s using following standard conditions: Default draw speed: 200 µl/min Default eject speed: 200 µl/min Injection volume: 5 µl
Carry-Over Typically < 0.01 % using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 µm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 µl caffeine (1 mg/ml), 5 µl water to test carryover Outside wash of needle before injection: 20 sec with water using flush port

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Agilent 1200 HPLC G1379B Degasser Agilent 1200 HPLC G1379B Degasser

Agilent 1200 HPLC G1379B Degasser Specifications

Maximum Flow Rate 0 to 5 ml/min per channel
Number of Channels 4
Internal Volume Per Channel Typically 1ml per channel
Materials in Contact with Solvent PTFE, FEP, PEEK
pH Range 1 to 14

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Agilent 1200 HPLC G1321A FLD Detector Agilent 1200 HPLC G1321A FLD Detector

Agilent 1200 HPLC G1321A FLD Detector Specifications

Detection Type Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications

10 fg Anthracene, Ex=250 nm, Em=400 nm

RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)

RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime

Excitation Monochromator

Range: settable 200 - 700 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: settable 280 - 900 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Wavelength Characteristic

Repeatability: ± 0.2 nm

Accuracy: ± 3 nm setting

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Agilent 1200 HPLC G1364C Fraction Collector Agilent 1200 HPLC G1364C Fraction Collector

Agilent 1200 HPLC G1364C Fraction Collector Specifications

Trigger Modes

Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)

Operating Modes

Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste

Test Tube/Plate Sizes

Minimum 48 mm to 75 mm maximum

Maximum Tube Volume

ca. 45 ml

Maximum Flow Rate

100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)

Delay Volumes [μl]

Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)

Diverter valve: ~15

Diverter valve to needle: ~110

Needle: -~5

Delay Calibration Sensor

Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode

Diverter Valve

3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar

Maximum Capacity

3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve

Interfaces

Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves

Safety Features

Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

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Agilent 1200 HPLC G1316A Column Compartment Agilent 1200 HPLC G1316A Column Compartment

Agilent 1100 HPLC G1316A Column Compartment Specifications

Temperature Range 10°C below ambient to 80°C
Temperature Stability ± 0.15°C
Temperature Accuracy ± 0.8°C
Column Capacity Three 30 cm columns
Warm-Up / Cool-Down Time

5 minutes from ambient to 40°C

10 minutes from 40°C to 20°C

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Agilent 1200 G1330B ALS Therm Agilent 1200 G1330B ALS Therm

Agilent 1200 G1330B ALS Specifications

Temperature Range 4°C to 40°C
Temperature Accuracy at Ambient Temperatures (< 25 °C and humidity < 50%) -1°C to +4°C at a setpoint of 4 °C
Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 60%) -1°C to +5°C at a setpoint of 4 °C
Weight 46 lbs. (20.7 kg)

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Agilent 1200 HPLC G1365B MWD Detector Agilent 1200 HPLC G1365B MWD Detector

Agilent 1200 HPLC G1365B MWD Detector Specifications

Detection Type 1024-element photodiode array
Light Source Deuterium and tungsten lamps
Wavelength Range 190 - 950 nm
Short Term Noise (ASTM) Single and Multi-Wavelength ± 1 x 10-5 AU, at 254 and 750 nm
Drift 2 x 10-3 AU/hr at 254 nm
Linear Absorbance Range > 2 AU (upper limit)
Wavelength Accuracy ± 1 nm
Wavelength Bunching 1 - 400 nm, programmable in 1 nm steps
Slit Width 1, 2, 4, 8, 16 nm
Diode Width < 1 nm

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Agilent 1200 HPLC G1315D DAD Detector Agilent 1200 HPLC G1315D DAD Detector

Agilent 1200 HPLC G1315D Diode Array Detector Specifications

Detection Type 1024-element photodiode array
Light Source Deuterium and tungsten lamps
Wavelength Range 190 - 950 nm
Short Term Noise (ASTM) Single and Multi-Wavelength ± 1 x 10-5 AU, at 254 and 750 nm
Drift 2 x 10-3 AU/h at 254 nm
Linear Absorbance Range > 2 AU (upper limit)
Wavelength Accuracy ± 1 nm
Wavelength Bunching 1 - 400 nm
Slit Width 1, 2, 4, 8, 16 nm
Diode Width < 1 nm

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Agilent 1200 HPLC G1362A RID Detector Agilent 1200 HPLC G1362A RID Detector

Agilent 1200 HPLC G1362A RID Detector Specifications

Detection Type Refractive Index
Refractive Index Range 1.00 to 1.75 RIU, calibrated
Measurement Range ± 600 x 10-6 RIU
Short Term Noise < ± 2.5 x 10-9 RIU
Drift < 200 x 10-9 RIU/h
Valves Automatic purge and automatic solvent recycle

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Agilent 1200 HPLC G1365D MWD Detector Agilent 1200 HPLC G1365D MWD Detector

Agilent 1200 HPLC G1365D MWD Detector Specifications

Detection Type 1024-element photodiode array
Light Source Deuterium and tungsten lamps
Wavelength Range 190 - 950 nm
Short Term Noise (ASTM) Single and Multi-Wavelength ± 1 x 10-5 AU, at 254 and 750 nm
Drift 2 x 10-3 AU/hr at 254 nm
Linear Absorbance Range > 2 AU (upper limit)
Wavelength Accuracy ± 1 nm
Wavelength Bunching 1 - 400 nm, programmable in 1 nm steps
Slit Width 1, 2, 4, 8, 16 nm
Diode Width < 1 nm

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Agilent 1200 HPLC G1316C Column Compartment Agilent 1200 HPLC G1316C Column Compartment

Agilent 1200 HPLC G1316C Column Compartment Specifications

Temperature Range Ambient -10°C to 100°C
Temperature Stability ± 0.05°C
Temperature Accuracy ± 0.8°C
Column Capacity Three 30 cm columns
Warm-Up / Cool-Down Time 5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C

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Agilent 1200 HPLC G1322A Degasser Agilent 1200 HPLC G1322A Degasser

Agilent 1200 HPLC G1322A Degasser

Maximum Flow Rate 10 ml/min per channel
Number of Channels 4
Internal Volume Per Channel Typically 12ml per channel
Materials in Contact with Solvent PTFE, PEEK
pH Range 1 to 14

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