Maximum RT-PCR Ramp Rate

Applied Biosystems 7500 Real-Time PCR System

Applied Biosystems 7500 Real-Time PCR System Specifications

Block Configuration	96-Well (20 to 100 uL reactions)
Temperature Range	4°C to 100°C
Temperature Uniformity	± 0.5°C (30 seconds after clock start)
Temperature Accuracy	± 0.25°C of setpoint/display temperature (measured at 3 minutes after clock start)
Maximum Ramp Rate	2.5°C/second
Run Time	< 2 hours
Optical System	CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range	9 logs of linear dynamic range
Sensitivity	Detection of 1 copy of template in 50 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing	Up to 5 targets per well
Dimensions (W x D x H)	13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight	75 lb. (34 kg)
Electrical Requirements	100-240V, 50/60Hz, 1080W

Our Price: $7,495.00

Applied Biosystems 7500 Fast Real-Time PCR System

Applied Biosystems 7500 Fast Real-Time PCR System Specifications

Block Configuration	96-Well Fast (5 to 30 uL reactions, optimized for 10 uL reactions)
Temperature Range	4°C to 100°C
Temperature Uniformity	± 0.5°C (30 seconds after clock start)
Temperature Accuracy	± 0.25°C of setpoint/display temperature (measured at 3 minutes after clock start)
Maximum Ramp Rate	5.5°C/second
Run Time	< 30 minutes
Optical System	CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range	9 logs of linear dynamic range
Sensitivity	Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing	Up to 5 targets per well
Dimensions (W x D x H)	13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight	75 lb. (34 kg)
Electrical Requirements	100-240V, 50/60Hz, 1080W

Our Price: $8,995.00

Biorad CFX Connect Real-Time PCR System

Biorad CFX Connect Real-Time PCR System Specifications

Block Configuration	96-Well (1 to 50 uL reactions, recommended for 10 to 25 uL reactions)
Temperature Range	0°C to 100°C
Temperature Uniformity	± 0.4°C well-to-well within 10 seconds of arrival at 90°C
Temperature Accuracy	± 0.2°C of programmed target at 90°C
Maximum Ramp Rate	5°C/second
Average Ramp Rate	3.3°C/second
Gradient Operational Range	30°C to 100°C
Gradient Programmable Span	1°C to 24°C
Scan Time	12 seconds (all channels), 3 seconds (FAM only)
Excitation Source	3 filtered LEDs
Detection Channels	3 filtered photodiodes
Range of Excitation/Emission Wavelengths	450 to 580 nm
Dynamic Range	10 orders of magnitude
Sensitivity	Detects 1 copy of target sequence in human genomic DNA
Multiplexing	Up to 2 targets per well
Dimensions (W x D x H)	13 x 18 x 14 in. (33 x 46 x 36 cm)
Weight	47 lb. (21 kg)
Electrical Requirements	100-240V, 50/60Hz

Our Price: $9,995.00

Applied Biosystems 7500 Fast Dx Real-Time PCR System

Applied Biosystems 7500 Fast Dx Real-Time PCR System Specifications

Block Configuration	96-Well Fast (10 to 30 uL reactions, optimized for 10 uL reactions)
Sample Format	Fast 96-Well plates (100 uL) or 8-tube strips (100 uL)
Temperature Range	4°C to 99.9°C
Temperature Uniformity	± 1°C
Temperature Accuracy	± 0.5°C
Sample Ramp Rate	Fast mode: 3.5°C/sec Standard mode: 1.6°C/sec
Peak Block Heating Rate	5.5°C/second
Run Time	< 40 minutes
Optical System	CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range	9 logs of linear dynamic range
Sensitivity	Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing	Up to 5 targets per well
Dye Compatibility	FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual)
Passive Reference Dyes	ROX dye or any calibrated dye (use of a passive reference dye is optional)
Dimensions (W x D x H)	13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight	75 lb. (34 kg)
Electrical Requirements	100-240V, 50/60Hz, 1080W

Our Price: $9,995.00

Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block

Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block Specifications

Block Configuration	384-Well (5 to 20 uL reactions)
Maximum Ramp Rate	6.0°C/second
Average Sample Ramp Rate	2.92°C/second
Temperature Uniformity	± 0.4°C
Run Time	Less than 30 minutes
Heating and Cooling Method	Peltier
Excitation (Light Source)	Bright white LED
Filters or Colors	5 coupled filters
Excitation and Detection Range	450 to 650 nm and 500 to 700 nm
Multiplexing	Up to 5 targets
Dynamic Range	10 logarithmic units
Sensitivity (Resolution)	Detect differences as small as 1.5-fold in target quantities in a singleplex reaction
Data Acquisition	Whole-plate imaging
Onboard Memory	10 GB, which translates to approximately 2,000 to 5,000 run files

Our Price: $14,995.00

Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block

Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block Specifications

Block Configuration	96-Well (10 to 100 uL reactions)
Maximum Ramp Rate	6.5°C/second
Average Sample Ramp Rate	3.66°C/second
Temperature Uniformity	± 0.4°C
Run Time	Less than 30 minutes
Heating and Cooling Method	Peltier
Excitation (Light Source)	Bright white LED
Filters or Colors	6 decoupled filters
Excitation and Detection Range	450 to 680 nm and 500 to 730 nm
Multiplexing	Up to 6 targets
Dynamic Range	10 logarithmic units
Sensitivity (Resolution)	Detect differences as small as 1.5-fold in target quantities in a singleplex reaction
Data Acquisition	Whole-plate imaging
Onboard Memory	10 GB, which translates to approximately 2,000 to 5,000 run files
Electrical Requirements	100-240V, 50/60Hz

Our Price: $14,995.00

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 384-Well Sample Block

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 384-Well Sample Block Specifications

Block Configuration	384-well
Block Change Design	Block change from front in less than 1 minute; no tools required
Ramp-Up Rate	3.5°C/second
Ramp-Down Rate	3.0°C/second
Well-to-Well Variability	± 0.25°C
Excitation Source	OptiFlex system lamp
Detection Channels	Excitation: 455 to 672 nm Emission: 505 to 723 nm
Dimensions (H x W x D)	29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight	154.3 lb. (70 kg)
Electrical Requirements	100-240V, 50/60Hz, 12.5A

Our Price: $19,595.00

Sale Price: $17,295.00

Money Saved: $2,300.00

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 96-Well Fast Sample Block

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 96-Well Fast Sample Block Specifications

Block Configuration	96-well Fast
Block Change Design	Block change from front in less than 1 minute; no tools required
Ramp-Up Rate	6.5°C/second
Ramp-Down Rate	6.0°C/second
Well-to-Well Variability	± 0.25°C
Excitation Source	OptiFlex system lamp
Detection Channels	Excitation: 455 to 672 nm Emission: 505 to 723 nm
Dimensions (H x W x D)	29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight	154.3 lb. (70 kg)
Electrical Requirements	100-240V, 50/60Hz, 12.5A

Our Price: $19,795.00

Sale Price: $17,495.00

Money Saved: $2,300.00

Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block

Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block Specifications

Block Configuration	96-Well Fast (15 to 30 uL reactions)
Block Change Design	Block change from front in less than 1 minute; no tools required
Maximum Block Ramp Rate	3.0°C/second
Well-to-Well Variability	± 0.25°C
Run Time	30 minutes expected (96-Well Fast block)
Excitation Source	Enhanced OptiFlex system, white LED
Detection Channels	Decoupled: 6 emission, 6 excitation
Demonstrated Sensitivity	To 1 copy
Dynamic Range	To 9 logarithmic units
Resolution	As low as 1.5-fold change for singleplex reaction
Remote Monitoring	Available to monitor up to 15 networked instruments simultaneously
Dimensions (H x W x D)	29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight	154.3 lb. (70 kg)
Electrical Requirements	100-240V, 50/60Hz, 12.5A

Our Price: $20,995.00

Sale Price: $19,995.00

Money Saved: $1,000.00