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Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Real-Time PCR System

Applied Biosystems 7500 Real-Time PCR System Specifications

Block Configuration 96-Well (20 to 100 uL reactions)
Temperature Range 4°C to 100°C
Temperature Uniformity ± 0.5°C (30 seconds after clock start)
Temperature Accuracy ± 0.25°C of setpoint/display temperature (measured at 3 minutes after clock start)
Maximum Ramp Rate 2.5°C/second
Run Time < 2 hours
Optical System CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range 9 logs of linear dynamic range
Sensitivity Detection of 1 copy of template in 50 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing Up to 5 targets per well
Dimensions (W x D x H) 13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight 75 lb. (34 kg)
Electrical Requirements 100-240V, 50/60Hz, 1080W

Our Price: $7,495.00
Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block

Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block Specifications

Block Configuration 384-Well (5 to 20 uL reactions)
Block Change Design Block change from front of instrument in less than 1 minute; no secondary tools required
Run Time ~ 35 minutes for 384-Well
Demonstrated Sensitivity Down to 1 copy
Dynamic Range Up to 9 logs of linear dynamic range
Resolution Detect changes as little as 1.5-fold in target quantities in singleplex reaction
Excitation/Detection Wavelengths

6 Excitation: 450 to 670 nm

6 Emission: 500 to 720 nm
Data Export Format

Fully configurable by user
Dimensions (W x D x H) 21 x 25 x 25.4 in. (53.3 x 63.5 x 64.5 cm)
Weight 148 lb. (66.9 kg)
Electrical Requirements 100-240V, 50/60Hz, 12.5A

Our Price: $8,995.00
Sale Price: $7,995.00
Money Saved: $1,000.00
Applied Biosystems 7500 Fast Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR System

Applied Biosystems 7500 Fast Real-Time PCR System Specifications

Block Configuration 96-Well Fast (5 to 30 uL reactions, optimized for 10 uL reactions)
Temperature Range 4°C to 100°C
Temperature Uniformity ± 0.5°C (30 seconds after clock start)
Temperature Accuracy ± 0.25°C of setpoint/display temperature (measured at 3 minutes after clock start)
Maximum Ramp Rate 5.5°C/second
Run Time < 30 minutes
Optical System CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range 9 logs of linear dynamic range
Sensitivity Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing Up to 5 targets per well
Dimensions (W x D x H) 13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight 75 lb. (34 kg)
Electrical Requirements 100-240V, 50/60Hz, 1080W

Our Price: $8,995.00
Biorad CFX Connect Real-Time PCR System Biorad CFX Connect Real-Time PCR System

Biorad CFX Connect Real-Time PCR System Specifications

Block Configuration 96-Well (1 to 50 uL reactions, recommended for 10 to 25 uL reactions)
Temperature Range 0°C to 100°C
Temperature Uniformity ± 0.4°C well-to-well within 10 seconds of arrival at 90°C
Temperature Accuracy ± 0.2°C of programmed target at 90°C
Maximum Ramp Rate 5°C/second
Average Ramp Rate 3.3°C/second
Gradient Operational Range 30°C to 100°C
Gradient Programmable Span 1°C to 24°C
Scan Time 12 seconds (all channels), 3 seconds (FAM only)
Excitation Source 3 filtered LEDs
Detection Channels 3 filtered photodiodes
Range of Excitation/Emission Wavelengths 450 to 580 nm
Dynamic Range 10 orders of magnitude
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Multiplexing Up to 2 targets per well
Dimensions (W x D x H) 13 x 18 x 14 in. (33 x 46 x 36 cm)
Weight 47 lb. (21 kg)
Electrical Requirements 100-240V, 50/60Hz

Our Price: $9,995.00
Applied Biosystems 7500 Fast Dx Real-Time PCR System Applied Biosystems 7500 Fast Dx Real-Time PCR System

Applied Biosystems 7500 Fast Dx Real-Time PCR System Specifications

Block Configuration 96-Well Fast (10 to 30 uL reactions, optimized for 10 uL reactions)
Sample Format Fast 96-Well plates (100 uL) or 8-tube strips (100 uL)
Temperature Range 4°C to 99.9°C
Temperature Uniformity ± 1°C
Temperature Accuracy ± 0.5°C
Sample Ramp Rate Fast mode: 3.5°C/sec
Standard mode: 1.6°C/sec
Peak Block Heating Rate 5.5°C/second
Run Time < 40 minutes
Optical System CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range 9 logs of linear dynamic range
Sensitivity Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing Up to 5 targets per well
Dye Compatibility FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual)
Passive Reference Dyes ROX dye or any calibrated dye (use of a passive reference dye is optional)
Dimensions (W x D x H) 13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight 75 lb. (34 kg)
Electrical Requirements 100-240V, 50/60Hz, 1080W

Our Price: $9,995.00
Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block

Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block Specifications

Block Configuration 384-Well (5 to 20 uL reactions)
Maximum Ramp Rate 6.0°C/second
Average Sample Ramp Rate 2.92°C/second
Temperature Uniformity ± 0.4°C
Run Time Less than 30 minutes
Heating and Cooling Method Peltier
Excitation (Light Source) Bright white LED
Filters or Colors 5 coupled filters
Excitation and Detection Range 450 to 650 nm and 500 to 700 nm
Multiplexing Up to 5 targets
Dynamic Range 10 logarithmic units
Sensitivity (Resolution) Detect differences as small as 1.5-fold in target quantities in a singleplex reaction
Data Acquisition Whole-plate imaging
Onboard Memory 10 GB, which translates to approximately 2,000 to 5,000 run files

Our Price: $14,995.00
Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block

Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block Specifications

Block Configuration 96-Well (10 to 100 uL reactions)
Maximum Ramp Rate 6.5°C/second
Average Sample Ramp Rate 3.66°C/second
Temperature Uniformity ± 0.4°C
Run Time Less than 30 minutes
Heating and Cooling Method Peltier
Excitation (Light Source) Bright white LED
Filters or Colors 6 decoupled filters
Excitation and Detection Range 450 to 680 nm and 500 to 730 nm
Multiplexing Up to 6 targets
Dynamic Range 10 logarithmic units
Sensitivity (Resolution) Detect differences as small as 1.5-fold in target quantities in a singleplex reaction
Data Acquisition Whole-plate imaging
Onboard Memory 10 GB, which translates to approximately 2,000 to 5,000 run files
Electrical Requirements 100-240V, 50/60Hz

Our Price: $14,995.00
Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 384-Well Sample Block Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 384-Well Sample Block

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 384-Well Sample Block Specifications

Block Configuration 384-well
Block Change Design Block change from front in less than 1 minute; no tools required
Ramp-Up Rate 3.5°C/second
Ramp-Down Rate 3.0°C/second
Well-to-Well Variability ± 0.25°C
Excitation Source OptiFlex system lamp
Detection Channels

Excitation: 455 to 672 nm

Emission: 505 to 723 nm

Dimensions (H x W x D) 29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight 154.3 lb. (70 kg)
Electrical Requirements 100-240V, 50/60Hz, 12.5A

Our Price: $19,595.00
Sale Price: $17,295.00
Money Saved: $2,300.00
Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 96-Well Fast Sample Block Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 96-Well Fast Sample Block

Applied Biosystems QuantStudio 7 Flex Real-Time PCR System w/ 96-Well Fast Sample Block Specifications

Block Configuration 96-well Fast
Block Change Design Block change from front in less than 1 minute; no tools required
Ramp-Up Rate 6.5°C/second
Ramp-Down Rate 6.0°C/second
Well-to-Well Variability ± 0.25°C
Excitation Source OptiFlex system lamp
Detection Channels

Excitation: 455 to 672 nm

Emission: 505 to 723 nm

Dimensions (H x W x D) 29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight 154.3 lb. (70 kg)
Electrical Requirements 100-240V, 50/60Hz, 12.5A

Our Price: $19,795.00
Sale Price: $17,495.00
Money Saved: $2,300.00
Applied Biosystems QuantStudio 12K Flex Real-Time PCR System Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block

Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block Specifications

Block Configuration 96-Well Fast (15 to 30 uL reactions)
Block Change Design Block change from front in less than 1 minute; no tools required
Maximum Block Ramp Rate 3.0°C/second
Well-to-Well Variability ± 0.25°C
Run Time 30 minutes expected (96-Well Fast block)
Excitation Source Enhanced OptiFlex system, white LED
Detection Channels Decoupled: 6 emission, 6 excitation
Demonstrated Sensitivity To 1 copy
Dynamic Range To 9 logarithmic units
Resolution As low as 1.5-fold change for singleplex reaction
Remote Monitoring Available to monitor up to 15 networked instruments simultaneously
Dimensions (H x W x D) 29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight 154.3 lb. (70 kg)
Electrical Requirements 100-240V, 50/60Hz, 12.5A

Our Price: $20,995.00
Sale Price: $19,995.00
Money Saved: $1,000.00