UV - Vis Absorbance Readers
Absorbance: 200 to 1000 nm
Fluorescence: 250 to 850 nm (Excitation) / 250 to 850 nm (Emission)
Luminescence: 250 to 850 nm
Absorbance: ≤ 4.0 nm
Fluorescence: 9.0 nm (Excitation) / 15 nm (Emission)
Absorbance: Monochromator, ± 1 nm
Fluorescence: Dual Monochromators, ± 1 nm
Fluorescence: 250 to 850 nm (Excitation) / 360 to 850 nm (Emission)
Fluorescence: 9.0 nm
Fluorescence: 250 to 850 nm (Excitation & Emission)
Absorbance: 200 to 999 nm
Fluorescence: 300 to 700 nm (Excitation & Emission)
Luminescence: 300 to 700 nm
Fluorescence: Filter Based
Absorbance Specifications
Light Source
Xenon flash lamp
Wavelength Selection
Double monochromator
Wavelength Range
230 to 999 nm, in 1 nm increments
Bandpass
2 nm (230-285 nm), 4 nm (>285 nm)
Pathlength Correction
Yes
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
± 0.2 nm
Dynamic Range
0 to 4.0 OD
Photometric Resolution
0.0001 OD
Photometric Accuracy
< 1% at 2.0 OD typical
Photometric Linearity
< 1% from 0 to 3.0 OD
Photometric Repeatability
< 0.5% at 2.0 OD
Reading Speed
96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity Specifications
Detector
Dedicated red shifted PMT
Double grating monochromators (top read only)
250 to 900 nm
5 decades
Sensitivity (Top Read Only)
Fluorescein 2 pM typical (0.2 fmol/well, 384-well plate)
Luminescence Specifications
< 6 decades
Sensitivity
10 amol ATP typical (flash)
Detection System
Low noise PMT
300 to 700 nm