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Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader
Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader
 
 



Our Price: $18,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 10 to 15 Business Days
Included Documentation Molecular Devices Absorbance and Fluorescence Validation Report

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Description
Recertification Protocol
Technical Specification Sheet
 

Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader



Wavelength Range
200 to 1000 nm

Wavelength Bandwidth
≤ 4.0 nm full width half maximum

Wavelength Accuracy
± 2.0 nm across wavelength range

Features
  • Supports absorbance (UV-Vis), fluorescence intensity, and luminescence detection modes
  • Dual monochromators for precise wavelength selection with 1 nm increments
  • Top-read and bottom-read modes for enhanced sensitivity in solution and cell-based assays
  • PathCheck technology ensures accurate pathlength correction for absorbance measurements
  • Integrated temperature control from ambient +2°C to 60°C with ±1°C accuracy
  • Dedicated luminescence photomultiplier tube (PMT) for low background noise
  • Compatible with 6-, 12-, 24-, 48-, 96-, and 384-well microplates
  • Robotics-compatible design for automation in high-throughput applications
  • Xenon flash lamp for reliable and consistent light source performance
  • Shaker with programmable timing from 0 to 999 seconds

The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. This FI, Abs, and Lumi microplate reader with a built-in cuvette port allows for easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays.

The SpectraMax M3 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The SpectraMax M3 features top-read and bottom-read modes, resulting in greater sensitivity for solution and cell-based assays. Within endpoint and kinetic measurement protocols, up to four wavelength pairs can be read at a time, allowing for improved efficiency for FRET assays. For Luminescence, the SpectraMax M3 utilizes a dedicated luminescence photomultiplier tube (PMT), providing the maximum signal and lowest background noise as possible for glow luminescence report gene assays. Key Features: dual mode cuvette port, robotics compatible, multilabel, dual monochromators, and PathCheck.

Applications include: DNA, RNA, and protein quantitation and purity; PicoGreen, NanoOrange, and Bradford; ELISAs and enzyme kinetics; drug dissolution profiles; live or dead viability or cytotoxicity assays; Caspase-3 and protease assays; cAMP assays using CatchPoint Assay Kits; and low volume applications.


All of our equipment is fully tested to perform at factory specifications before being shipped.


INCLUDED:
The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader includes:
  • > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 6.3
  • > Interface Cable
  • > Molecular Devices Absorbance and Fluorescence Validation Report
  • > Top Read Plate
  • > Power Cord
  • > Operator's Guide
  • > Full 180 Day Parts and Labor Warranty

Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Specifications

Absorbance Photometric Performance

Wavelength Selection

Monochromator, tunable in 1 nm increments

Wavelength Range

200 to 1000 nm

Wavelength Bandwidth

4.0 nm full width half maximum

Wavelength Accuracy

± 2.0 nm across wavelength range

Wavelength Repeatability

± 0.2 nm

Photometric Range

0.000 to 4.000 OD

Photometric Resolution

0.001 OD

Photometric Accuracy (Microplate)

< ± 0.006 OD ± 1%, 0 to 2 OD

Photometric Accuracy (Cuvette)

< ± 0.005 OD ± 1%, 0 to 2 OD

Photometric Precision

< ± 0.003 OD ± 1%, 0 to 2 OD

Baseline Flatness

< 0.001 OD

Stray Light

< 0.05% @ 230 nm

Fluorescence Intensity Performance

Dual Monochromators

1 nm increment selection

EX Wavelength Range

250 to 850 nm

EM Wavelength Range

250 to 850 nm

Wavelength Bandwidth (EX, EM)

9 nm

Top-Read Optimized Sensitivity

1 pM (fluorescein) in 96 wells
1.5 pM (fluorescein) in 384 wells

Top-Read Guaranteed Sensitivity

< 5 pM (fluorescein) in 96 wells or cuvette
< 20 pM in 384 wells

Luminescence Photometric Performance

Sensitivity

< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)

Wavelength Range

250 to 850 nm

Crosstalk

< 0.5% in 96- and 384- well microplates

Dynamic Range

Optimized Sensitivity

43 pM ATP in 96 wells

Guaranteed Sensitivity

75 pM ATP in 96 wells

General Photometric Performance

Plate Formats

6, 12, 24, 48, 96, 384 wells

Light Source

Xenon flash lamp (1 joule/flash)

Detectors

2 Photomultiplier tubes (PMT)

Read Time

96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.

384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.

Shaker Time

0 to 999 seconds

Temperature Control

Ambient +2°C to 60°C

Temperature Uniformity

< 1°C at 37°C set point

Temperature Accuracy

± 1°C at 37°C set point

General Specifications

Dimensions (H x W x D)

21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)

Weight

16.4 kg (36 lbs)


Computer Specifications

Model

Hewlett-Packard

Processor

Intel Core i5-4300U CPU

RAM

8GB

Hard Drive

240GB Internal SSD

Operating System

Windows 10 Professional, 64-bit

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