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GE AKTA Purifier 100 FPLC System w/ UV-900 Detector
Flow Rate Range 0.01 to 100 ml/min
Pressure Range 0 to 10 Mpa
Wavelength Range 190 to 700 nm
- UV-900 Detector provides precise detection of analytes, enhancing the accuracy of chromatography results.
- Box-900 and P-900 Pump with 100ml Pump Heads for dependable fluid delivery.
- The PHC-900 Monitor Ensures exact pH monitoring, vital for sensitive chromatographic separations.
- Guarantees thorough mixing of samples with use of M-925 mixer.
- Enables precise measurement of sample conductivity with use of conductivity flow cell.
The GE AKTA Purifier 100 FPLC System is versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides, and nucleic acids by FPLC. A KTA Purifier 100 is designed for purification of microgram to milligram quantities of protein, operating at flow rates of 0.001 to 10 ml/min at pressures of 0 to 25 MPa. The GE AKTA is in excellent condition and has passed all tests for full functionality. These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing PHC-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing motor valves, and testing the Frac-920 for functionality through manual commands. (Additional cost for pH Probe and Holder, please inquire).
The GE AKTA Purifier 100 FPLC System w/ UV-900 Detector includes:
- > Box-900
- > P-900 Pump w/ 100ml Pump Heads
- > P-960 Sample Pump
- > UV-900 Detector
- > New UV Lamp
- > PHC-900 Monitor
- > INV-907 Valve
- > PV-908 Valves (2)
- > M-925 Mixer w/ 2ml Mixing Chamber
- > Conductivity Flow Cell
- > CU-950 Communication Module
- > Frac-920 Fraction Collector with Tube Rack
- > Dell Windows 7 Datastation with installed Unicorn version 5.31 software
- > Flat Screen Monitor
- > Installation Calibration Report
- > Communication Cables
- > Tubing, Power Cords
- > Full 180 Day Parts & Labor Warranty
GE AKTA Purifier 100 FPLC System Specifications
P-900
Specifications
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Flow Rate Range
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Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min
Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min
Double mode: 0.01 to 200 ml/min in steps of 10 ul/min
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Pressure Range
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0 to 10 MPa (100 bar, 1450 psi)
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pH Stability Range
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1 to 13 (1 to 14 with <1 day exposure)
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Viscosity
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Max 5 cP
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Flow Rate Accuracy
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± 2% or 20 ul/min, whichever is greater
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Flow Rate Reproducibility
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Flow rate ≥ 0.5 ml/min: rsd < 0.5%
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Gradient Composition
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Accuracy: < ±1% at 0.05 to 10 ml/min
Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min
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Pressure Sensor
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Range: 0 to 27.5 MPa
Offset error: < 0.05 MPa
Scale error: < ±2%
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Total Volume Between Inlet and Outlet
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< 800 ul/module
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UV-900
Specifications
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Wavelength range
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190–700 nm in steps of 1 nm,
3 wavelengths simultaneously
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Bandwidth
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4 nm
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Wavelength accuracy
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±2 nm
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Wavelength reproducibility
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< 500 ms (one cycle from 214 nm to
254 nm and back to 214 nm)
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Linearity
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< 2% deviation up to 2 AU at 260 nm with
Uracil at pH 2
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Static noise
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short term: < 6x10-5 AU at 230 nm
long term: < 6x10-5 AU at 230 nm
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Drift
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< 2x10-4 AU/h at 254 nm
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M-925
Specifications
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Max Flow Rate
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0.001 to 10 ml/min
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Max Pressure
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12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)
0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar,
3625 psi)
90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075
psi)
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pH Stability Range
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1 to 13 (1 to 14 with <1 day exposure)
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Viscosity
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Max 5 cP
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Internal Volume
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6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml
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Mixing Principle
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1 static chamber and 1 dynamic chamber
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Inlet and Outlet Tubing
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UNF 10 to 32 thread profile
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Frac-920
Specifications
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Capacity
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95 fractions in 10-18 mm tubes
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Depth
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400 mm
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Flow Rate
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< 100 ml/min
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Height
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250 mm
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Operating Temperature
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4° to 40°C
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pH Stability Long Term
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1 – 13
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pH Stability Short Term
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1 – 14
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Program Memory
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99 programs
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Rack Designation
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95 x 10-18 mm (Standard)
175 x 12 mm and 40 x 30 mm (Optional)
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Relative Humidity
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20 to 95% (non condensing)
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Time Mode
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0.01 to 9.999 min
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Volume Mode
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0.1 to 999 ml
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Computer Specifications
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Model
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Dell Optiplex 7010 Desktop PC
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Processor
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Intel Core i5-3470 Processor 3.2GHz
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RAM
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8GB
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Hard Drive
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250GB Internal SSD
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Operating System
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Windows 7 Professional, 64-bit
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- Inspect All Displays and Replace Any Dim/Dead Pixels
- Inspect Internal Components Of P-900, Uv-900/Upc-900 And Ph/C-900 and Replace any Malfunctioning Components
- Confirm All Components Are Connecting Via Uni-Net 1 & 2 Interfaces
- Check And Replace Any Uninet Cables Malfunctioning
- Check Internal Components Of M-925, Inv-907, Pv-908, Pv-907 To Confirm Full Functionality
- Replace All Peek Tubing with New Oem Tubing and Check All Components Within Flow Path for Clogs
- Confirm Software Successfully Interfaces with The Fplc System
- Replace Pump Piston Seals, Wash Seals, And Seal Wash Tubing Along with Ultrasonic Cleaning of Each Check Valve in The Pumps Flow Path
- Calibrate Pressure and Flow of Both P-900 Pump And P-960/950 Pump (If Applicable)
- Replace Uv Lamp and Run Internal Diagnostic Tests to Confirm Full Functionality
- Install New Ph Probe Along and Verified Conductivity Cell
- Calculate Delay Volume and Update Software
- Clean/Replace Drop Sensor, Accumulator Wash, And Height Adjuster.
- Verify Fraction Collector Alignment Calibration With 15Ml, 50Ml, And Microplate Receptacles
- Run Installation Test and Document Findings
To View More of Our Recertification Protocol Click Here
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Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.
All Lab Equipment is Fully Recertified and Tested
Most Pieces Ship within 1 to 3 Business Days
All Products are Fully Warrantied
Trained Customer and Technical Support
Industry Leading Overall Customer Satisfaction
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