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GE AKTA FPLC Explorer 100 System
GE AKTA FPLC Explorer 100 System
 
 



Our Price: $27,090.00
Sale Price: $22,995.00
Money Saved: $4,095.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 6 to 8 Business Days
Included Documentation: Installation Calibration Report

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Quantity in Stock:2

Product Code: GE-EX1002
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Description
Recertification Protocol
Technical Specification Sheet
 

GE AKTA FPLC Explorer 100 System



Wavelength Range
190–700 nm in steps of 1 nm

Wavelength Accuracy
±2 nm

Max. Flow Rate
100 ml/min

Features
  • Supports size exclusion, ion exchange, and affinity chromatography.
  • Six interchangeable mixing chambers, accommodating flow rates from 0.001 to 100 ml/min, and can handle pressures up to 35 MPa.
  • Ensures smooth operation and integration with industry-standard Unicorn 5.31 software.
  • Allows for fast pH optimization, enhancing the efficiency of purification processes.
  • Ideal for protein purification in research and biotechnology, including proteomics, drug discovery, biochemistry, and molecular biology.


The GE ÄKTA FPLC Explorer 100 System is designed for a wide range of applications in research and biotechnology, catering to the diverse needs of scientists working on protein purification. Its versatility allows for use in size exclusion, ion exchange, and affinity chromatography, making it ideal for studying protein interactions, characterizing protein structure, and isolating proteins for further downstream analysis. This system is particularly useful in fields such as proteomics, drug discovery, biochemistry, and molecular biology, offering reliable performance and accurate results in the purification of recombinant proteins, antibodies, and protein complexes. Choose the ÄKTA Explorer 100 System for a comprehensive and adaptable solution for your protein purification needs.

The GE ÄKTA FPLC Explorer 100 System is a versatile and powerful solution for protein purification. This system features a UV-900 Detector with a wavelength range of 190-700 nm, offering simultaneous detection at three wavelengths with 4 nm bandwidth, and a flow cell capable of handling up to 100 ml/min with a maximum pressure of 2 MPa. The P-900 Pump ensures accurate and reproducible flow rates ranging from 0.01 to 100 ml/min in both isocratic and gradient modes. The M-925 Mixer, with six interchangeable mixing chambers, accommodates flow rates from 0.001 to 100 ml/min and can handle pressures up to 35 MPa, depending on the chamber. The Frac-950 Fraction Collector offers a wide range of fraction sizes and flow rates, with a pH stability range of 1 to 13. Trust the ÄKTA Explorer 100 System for efficient and reliable protein purification in your research and biotechnology applications.



This GE AKTA Explorer 100 FPLC System is in excellent condition and has passed all tests for full functionality. These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing operation of pressure sensor in P-900 and P-950, leak-testing P-900 and P-950, testing UV-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing functionality of the 925 Sensor, testing motor valves, and testing the Frac-950 for functionality through manual commands.

Configured setup for detection is UV Absorbance, pH, and Conductivity. Fraction collection is through the Frac-950.

INCLUDED:
The GE AKTA FPLC Explorer 100 System includes:
  • > Box-900
  • > P-900 Pump w/Switch Valves
  • > UV-900 Detector
  • > UV Flow Cell
  • > New UV Lamp
  • > pH/C-900 Monitor
  • > P-960 Sample Pump
  • > M-925 Mixer
  • > 2mL Mixing Chamber M-925
  • > Conductivity Cell
  • > pH Probe and Holder
  • > CU-950 Control Box
  • > Injection V1 INV-907
  • > Column V2 PV-908
  • > Column V3 PV-908
  • > Outlet V4 PV-908
  • > Sample V5 Valve PV-908
  • > Buffer Valve V6 IV-908
  • > Flow Direction Valve V7 INV-907
  • > FR-902 Flow Restrictor
  • > Inline Filter and Tubing
  • > Frac-950 Fraction Collector & Rack
  • > Dell Datastation running Windows 7 Pro with Unicorn version 5.31 software
  • > Flat Screen Monitor
  • > Installation Calibration Report
  • > Communication and Power Cables
  • > Full 180 Day Parts and Labor Warranty

GE AKTA FPLC Explorer 100 System Specifications

UV-900 Detector Specifications

Wavelength range

190–700 nm in steps of 1 nm,

3 wavelengths simultaneously

Bandwidth

4 nm

Wavelength accuracy

±2 nm

Wavelength reproducibility

< 500 ms (one cycle from 214 nm to

254 nm and back to 214 nm)

Linearity

< 2% deviation up to 2 AU at 260 nm with

Uracil at pH 2

Static noise

short term: < 6x10-5 AU at 230 nm

long term: < 6x10-5 AU at 230 nm

Drift

< 2x10-4 AU/h at 254 nm

Flow Cell

Optical path length 2 mm cell

2 mm, 2 µl cell volume

Optical path length 10 mm cell

10 mm, 8 µl cell volume

Max. flow rate

100 ml/min

Max. pressure

2 MPa (290 psi)

Temperature range

+4 to +40 °C (liquid)

Wetted material

PTFE (polytetrafluoroethylene)

PEEK (polyetheretherketone)

titanium (palladium alloy),

quartz (synthetic fused silica)

and titanium

P-900 Pump Specifications

Flow Rate Range

Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min

Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min

Double mode: 0.01 to 200 ml/min in steps of 10 ul/min

Pressure Range

0 to 10 MPa (100 bar, 1450 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Flow Rate Accuracy

± 2% or 20 ul/min, whichever is greater

Flow Rate Reproducibility

Flow rate ≥ 0.5 ml/min: rsd < 0.5%

Gradient Composition

Accuracy: < ±1% at 0.05 to 10 ml/min

Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min

Pressure Sensor

Range: 0 to 27.5 MPa

Offset error: < 0.05 MPa

Scale error: < ±2%

Total Volume Between Inlet and Outlet

M-925 Mixer Specifications

Max Flow Rate

0.001 to 100 ml/min

Max Pressure

12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)

0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi)

90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Internal Volume

6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml

Mixing Principle

1 static chamber and 1 dynamic chamber

Inlet and Outlet Tubing

UNF 10 to 32 thread profile

Frac-950 Fraction Collector Specifications

Max Flow Rate

100 ml/min

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Fraction Size

0.1 to 99999.99 ml (volume mode)

0.1 to 99999.99 min (time mode)

Standard Mode

u-liter plates, DropSync: 0 to 1 ml/min

12 mm rack, DropSync: 0 to 1.5 ml/min

18 and 30 mm racks, DropSync: 0 to 2 ml/min

18 and 30 mm racks, Accumulator: 15 to 100ml/min

Prep Mode 30 mm rack, Accumulator: 15 to 100 ml/min
Funnel-to-flask, Accumulator: 15 to 100 ml/min
250ml bottles, Accumulator: 15 to 100 ml/min
Environmental Conditions +4° to +40°C
20 to 95% relative humidity
84 to 106 kPa (840 to 1060 mbar) atmospheric pressure
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