GE AKTA FPLC Explorer 100 System

UV-900 Detector Specifications

Wavelength range

190–700 nm in steps of 1 nm,

3 wavelengths simultaneously

Bandwidth

4 nm

Wavelength accuracy

±2 nm

Wavelength reproducibility

< 500 ms (one cycle from 214 nm to

254 nm and back to 214 nm)

Linearity

< 2% deviation up to 2 AU at 260 nm with

Uracil at pH 2

Static noise

short term: < 6x10-5 AU at 230 nm

long term: < 6x10-5 AU at 230 nm

Drift

< 2x10-4 AU/h at 254 nm

Flow Cell

Optical path length 2 mm cell

2 mm, 2 µl cell volume

Optical path length 10 mm cell

10 mm, 8 µl cell volume

Max. flow rate

100 ml/min

Max. pressure

2 MPa (290 psi)

Temperature range

+4 to +40 °C (liquid)

Wetted material

PTFE (polytetrafluoroethylene)

PEEK (polyetheretherketone)

titanium (palladium alloy),

quartz (synthetic fused silica)

and titanium

P-900 Pump Specifications

Flow Rate Range

Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min

Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min

Double mode: 0.01 to 200 ml/min in steps of 10 ul/min

Pressure Range

0 to 10 MPa (100 bar, 1450 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Flow Rate Accuracy

± 2% or 20 ul/min, whichever is greater

Flow Rate Reproducibility

Flow rate ≥ 0.5 ml/min: rsd < 0.5%

Gradient Composition

Accuracy: < ±1% at 0.05 to 10 ml/min

Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min

Pressure Sensor

Range: 0 to 27.5 MPa

Offset error: < 0.05 MPa

Scale error: < ±2%

Total Volume Between Inlet and Outlet

M-925 Mixer Specifications

Max Flow Rate

0.001 to 100 ml/min

Max Pressure

12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)

0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi)

90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Internal Volume

6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml

Mixing Principle

1 static chamber and 1 dynamic chamber

Inlet and Outlet Tubing

UNF 10 to 32 thread profile

Frac-950 Fraction Collector Specifications

Max Flow Rate

100 ml/min

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Fraction Size

0.1 to 99999.99 ml (volume mode)

0.1 to 99999.99 min (time mode)

Standard Mode

u-liter plates, DropSync: 0 to 1 ml/min

12 mm rack, DropSync: 0 to 1.5 ml/min

18 and 30 mm racks, DropSync: 0 to 2 ml/min

18 and 30 mm racks, Accumulator: 15 to 100ml/min