Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth
9 nm
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Light Source
Xenon flash lamp
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
Absorbance Photometric Performance
Wavelength Selection
Monochromator, tunable in 1 nm increments
Wavelength Range
200 to 1000 nm
≤ 4.0 nm
Wavelength Accuracy
± 2.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0 to 4.0 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
Photometric Precision
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Photometric Performance
Dual Monochromators
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Detector
Photomultiplier tube (PMT)
Read Time
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
Time Resolved Fluorescence
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
360-850nm
Detection Limit
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Photomultiplier (R-3896)
96 < 15 sec, 384 < 45 sec
Ambient +4°C - 45°C
UV/Vis Photometric Performance
200 - 1000 nm
Monochromator, tunable in 1.0 nm increments
0 - 4.0 OD
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
General Specifications:
Xenon Flash Lamp
96 well (Abs 18 sec, FI 15 sec) 384 well (Abs 49 sec, FI 45 sec)
Ambient +4 °C to 45°C
± 1 °C @ 37°C
± 0.5°C @ 37°C set point
Power
110 - 120 Vac 50/60 Hz
UV/Vis Photometric Performance:
200 – 1000 nm
0 – 4.0 OD
1 nm increments
EX 250 – 850 nm
EM 360 – 850 nm
