Fluorescence Photometric Performance
Dual Monochromators
1nm increments
Wavelength Range
250-850nm
Bandwidth (Excitation & Emission)
9 nm
Time Resolved Fluorescence
Data Collection
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
Detection Limit
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Light Source
Xenon flash lamp
Detector
Photomultiplier (R-3896)
Read Time
96 < 27 sec, 384 < 45 sec
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C - 45°C
UV/Vis Photometric Performance
200 - 1000 nm
Wavelength Selection
Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth
≤ 4.0 nm
Wavelength Accuracy
± 2.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0 - 4.0 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (Microplate)
< ± 0.006 OD ± 1.0%, 0-2 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0-2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Photometric:
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M2e allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.
General Info
Dimensions (H x W x D)
8.6 x 22.8 x 15 in (22 x 58 x 38 cm)
Weight
35 lbs (16 kg)
Power Source
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
The Molecular Devices Spectramax Gemini is built on the Gemini platform delivering high quality with top and bottom reading, wavelength scanning. well scanning and more. The Gemini uses a Xenon flash lamp and has the ability to do full range scanning in 1nm increments. Measure 96 well plate in 15 seconds or less. Instrument also comes with built-in shaker and temperature control chamber.
Please Call: (603)-601-8511 or 
