Absorbance Specifications
Light Source
Xenon flash lamp
Wavelength Selection
Monochromator
Wavelength Range
230 to 999 nm, in 1 nm increments
Bandpass
2 nm (230 to 285 nm), 4 nm (> 285 nm)
Dynamic Range
0 to 4.0 OD
Photometric Resolution
0.0001 OD
Pathlength Correction
Yes
Photometric Accuracy
< 1% at 2.0 OD typical
< 3% at 3.0 OD typical
Photometric Repeatability
< 0.5% at 2.0 OD typical
Fluorescence Intensity Specifications
Tungsten halogen lamp
Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top)
Monochromators: 250 to 850 nm
Filters: 200 to 700 nm (850 nm option)
Monochromators: 5 decades
Filters/Mirrors: > 6 decades
Sensitivity
Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate)
Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)
Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
Luminescence Specifications
300 to 700 nm
< 6 decades
10 amol ATP typical (flash)
Fluorescence Polarization Specifications
Tungsten halogen
Deep blocking filters/dichroic mirrors (top)
400 to 700 nm (320 to 850 nm option)
3 mP at 1 nm fluorescein typical
Time-Resolved Fluorescence Specifications
High energy xenon flash lamp
200 to 700 nm (850 nm option)
Europium 60 fM typical with filters (6 amol/well in 384-well plate)
Double monochromator
2 nm (230-285 nm), 4 nm (>285 nm)
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
± 0.2 nm
Photometric Linearity
< 1% from 0 to 3.0 OD
< 0.5% at 2.0 OD
Reading Speed
96 wells: 11 seconds
384 wells: 22 seconds
Detector
Dedicated red shifted PMT
Double grating monochromators (top read only)
250 to 900 nm
5 decades
Sensitivity (Top Read Only)
Fluorescein 2 pM typical (0.2 fmol/well, 384-well plate)
Detection System
Low noise PMT
UV/Vis Photometric Performance:
200 ‚ 1000 nm
Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth
°¤ 4.0 nm
Wavelength Accuracy
± 2.0 nm
Wavelength Repeatability
Photometric Range
0 ‚ 4.0 OD
0.001 OD
Photometric Accuracy (Microplate)
< ± 0.006 OD ± 1.0%, 0-2 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0-2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Photometric:
Dual Monochromators
1 nm increments
EX 250 ‚ 850 nm
EM 360 ‚ 850 nm
Bandwidth (EX, EM)
9, 9 nm
Detection Limit
3.0 fmol/well FITC 200µl in 96 wells
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Fluorescence Photometric Performance
1 nm increment selection
EX Wavelength Range
250 to 850 nm
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
9 nm
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
Read Time
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C to 45°C
Temperature Uniformity
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
± 1° at 37°C
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
Time Resolved Fluorescence
50-1450 µ/sec, 200µ/sec increments
0.5/ well
Luminescence Performance
360-850nm
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Photomultiplier (R-3896)
96 < 15 sec, 384 < 45 sec
Ambient +4°C - 45°C
UV/Vis Photometric Performance
200 - 1000 nm
0 - 4.0 OD
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
General Specifications:
Xenon Flash Lamp
96 well (Abs 18 sec, FI 15 sec) 384 well (Abs 49 sec, FI 45 sec)
Ambient +4 °C to 45°C
± 1 °C @ 37°C
± 0.5°C @ 37°C set point
Power
110 - 120 Vac 50/60 Hz
