Fluorescence Photometric Performance
Dual Monochromators
1nm increments
Wavelength Range
250-850nm
Bandwidth (Excitation & Emission)
9 nm
Time Resolved Fluorescence
Data Collection
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
Detection Limit
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Light Source
Xenon flash lamp
Detector
Photomultiplier (R-3896)
Read Time
96 < 27 sec, 384 < 45 sec
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C - 45°C
UV/Vis Photometric Performance
200 - 1000 nm
Wavelength Selection
Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth
≤ 4.0 nm
Wavelength Accuracy
± 2.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0 - 4.0 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (Microplate)
< ± 0.006 OD ± 1.0%, 0-2 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0-2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Photometric:
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
The Molecular Devices Spectramax Gemini is built on the Gemini platform delivering high quality with top and bottom reading, wavelength scanning. well scanning and more. The Gemini uses a Xenon flash lamp and has the ability to do full range scanning in 1nm increments. Measure 96 well plate in 15 seconds or less. Instrument also comes with built-in shaker and temperature control chamber.
General Info
Dimensions (H x W x D)
8.6 x 22.8 x 15 in (22 x 58 x 38 cm)
Weight
35 lbs (16 kg)
Power Source
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M2e allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.
Please Call: (603)-601-8511 or 
