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Eppendorf Mastercycler X40 PCR Thermocycler
Thermal Range
4°C to 99°C
Ramping Rates
Heating: 3.3°C/sec Cooling: 1.5°C/sec
Memory
Up to 5,000 programs and 500 folders
- 12-column temperature gradient function enables rapid PCR optimization in a single run
- SafeLid® system adjusts automatically to different vessel heights for secure sealing and reduced evaporation
- Large 7-inch touchscreen interface simplifies navigation and program setup
- Remote monitoring and real-time alerts supported through connected software integration
- Intelligent lid preheating minimizes thermal shock and reduces nonspecific amplification
- Compatible with a wide range of vessel formats including tubes and 96-well plates
- Compact, energy-efficient design supports sustainability and quiet lab operation
The Eppendorf Mastercycler X40 Thermal Cycler is engineered to simplify and accelerate PCR workflows with a strong focus on usability, consistency, and flexibility. Its advanced temperature gradient feature allows researchers to test up to 12 different annealing or denaturation temperatures in a single run, making PCR optimization faster and more reliable. The integrated SafeLid system automatically adjusts to various vessel heights—ranging from tubes to plates—ensuring tight sealing, optimal heat transfer, and protection against evaporation.
User interaction is streamlined through the large 7-inch touchscreen interface, which offers intuitive navigation, detailed program setup, secure user access controls, and onboard documentation. Through integration with VisioNize Lab Suite, users can monitor runs remotely, receive real-time alerts, and manage instruments across the lab from a centralized platform—enhancing efficiency and traceability. The system’s intelligent lid preheating protocol minimizes thermal shock during startup, helping reduce nonspecific amplification and ensuring cleaner results. Quiet, compact, and energy-efficient, the Mastercycler X40 is designed for modern labs seeking reproducible PCR performance without added complexity. Its compatibility with a wide range of vessel formats, combined with smart features that support fast turnaround and minimal user intervention, make it a dependable solution for both routine protocols and advanced research applications.
All of our equipment is fully tested to perform at factory specifications before being shipped.
Eppendorf Mastercycler X40 PCR Thermocycler includes:
- > 96-Well Aluminum Block
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Eppendorf Mastercycler X40 PCR Thermocycler Specifications
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Thermal Range (Block)
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4°C to 99°C
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Thermal Range (Lid)
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37°C to 110°C
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Thermal Accuracy
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± 0.15°C
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Temperature Control Modes
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Fast, Intermediate, Standard, Safe
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Ramping Rates
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Heating: 3.3°C/sec
Cooling: 1.5°C/sec
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Sample Capacity
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96x 0.1 mL PCR tubes
96 x 0.2 ml PCR tubes
1x 96-well PCR plate (unskirted, semi-skirted, skirted), high-profile and low-profile
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Memory
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Up to 5,000 programs and 500 folders
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Gradient Spread
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30°C maximum
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Gradient Temperature Range
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30°C to 99°C
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Interfaces
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USB and Ethernet
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Heating Technology
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Three peltier elements
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Dimensions (W x D x H)
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9.7 × 15.2 × 9.1 in (24.5 × 38.5 × 23 cm)
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Weight
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15.98 lb. (7.25 kg)
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Electrical Requirements
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100 - 240V, 50/60Hz, 500W
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- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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