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Eppendorf 6377 Mastercycler Nexus X2 Thermal Cycler
Thermoblock Temperature Range
4°C to 99°C
Sample Capacity
64 / 32 x 2 ml
; 24 / 10 x 0.5 ml
- Asymmetrically divided aluminum block allows two independent PCR protocols to run in parallel
- Separate block sections support simultaneous processing of different assay types
- Intuitive graphic programming interface enables clear visualization and easy protocol setup
- Optional mouse support provides additional control flexibility
- Flexlid technology automatically adjusts lid height for different consumables
- Thermal sample protection helps maintain consistent reaction conditions
- Can be networked to control up to two additional Mastercycler units
- Compact footprint helps conserve valuable bench space
- USB, Ethernet, and CAN interfaces support connectivity and system integration
- Designed for consistent, reproducible PCR performance across daily workflows
The Eppendorf 6377 Mastercycler Nexus X2 Thermal Cycler is designed to provide flexible, efficient PCR workflows by allowing two independent assays to run in parallel on a single instrument. Its asymmetrically divided aluminum block combines a 32-well and a 64-well section, enabling smaller and larger assays to be processed simultaneously without compromise. This design supports efficient use of bench space while maintaining the consistency and reliability expected from the Mastercycler nexus family.
An intuitive graphic interface simplifies programming and operation, with clear visualization of protocols and optional mouse control for added convenience. The universal block accommodates PCR tubes, strips, and divisible plates, while the flexlid concept automatically adjusts lid height to ensure optimal contact and thermal protection across different consumable types. Built for connectivity and streamlined operation, the Mastercycler Nexus X2 supports email notifications and can be networked with up to two additional Mastercycler units, allowing multiple systems to be managed with the same user-friendly software. Together, these features make the Nexus X2 a versatile and dependable solution for laboratories running diverse PCR applications in parallel.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Eppendorf 6377 Mastercycler Nexus X2 Thermal Cycler includes:
- > Block
- > Calibration and Preventative Maintenance Report
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Eppendorf 6377 Mastercycler Nexus X2 Thermal Cycler Specifications
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Sample Capacity
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64 / 32 x 2 ml
24 / 10 x 0.5 ml
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Thermoblock Temperature Range
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4°C to 99°C
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Ramp Rate
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Heating: 3°C/s
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Thermoblock Temperature Homogeneity
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≤ 3°C at 35°C
≤ 4°C at 90°C
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Control Accuracy
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± 0.2°C
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Heated Lid Temperature Control Range
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37°C to 110°C
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Memory
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> 700 Programs
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Dimensions (W x D x H)
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9.8 x 16.1 x 13 in. (25 x 41 x 33 cm)
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Weight
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24.3 lb. (11 kg)
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Electrical Requirements
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100-240V, 50/60Hz, 700W
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- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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