Temperature Range
10° below ambient to 80° C
Temperature Stability
± 0.15°C
Temperature Accuracy
± 0.8°C
Column Capacity
Three 30cm columns
Warm-up/Cool Down
5 mins from ambient to 40°C
Dead Volume
3 ul left heat exchanger6 ul right heat exchanger
Dimensions (H x W x D)
5.5 x 16 x 17 in (14 x 41 x 43.5 cm)
Detection Type
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
Pulse frequency
296 Hz for single signal mode 74 Hz for spectral mode
Excitation Monochromator
Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm
Reference System:
in-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition:
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
Setable Flow Range
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range
0.1 to 5 ml/min
Flow Precision
≤ 0.07% RSD or ≤ 0.02 min SD
Flow Accuracy
± 1% or 10 ul/min
Pressure
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation
User-selectable, based on mobile phase compressibility
Hydraulic System
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Recommended pH Range
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range
1 to 99% or 5 ul/min per channel
Composition Precision
≤ 0.5% absolute
Composition Accuracy
≤ 0.15% RSD at 1 ml/min
Max Flow Rate
10 ml/min per channel
Reduced performance above 5 ml/min
Number of Channels
4
Internal Volume
1 ml/channel
Materials in Contact with Solvent
PTE, PEEK
pH Range
1 – 14
Analog Output (AUX)
For pressure monitoring, range 0 – 3 V
Dimensions (W x D x H)
13.5 x 17 x 3.1 in (345 x 435 x 80 mm)
Electrical Requirements
100-120 or 220-240 VAC, 50/60 Hz
Maximum Flow Rate
0-5 ml/min per channel
5-10 ml/min at reduced degassing performance
Ambient Operating Temperature
0° to 55°C
Ambient Non-operating Temperature
-40° to 70°C
Power Consumption
30 W
Line Voltage
100-120 220-240 VAC
Line Frequency
50 r 60 Hz
Weight
7kg (15.4 lbs)
13.5 x 17 x 3.1 in
