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Biotek Synergy HT Microplate Reader
Biotek Synergy HT Microplate Reader

Our Price: $10,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 1 to 2 Business Days
Included Documentation: ABS, FLD, and LUM Calibration Report

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Quantity in Stock:2

Product Code: B-SHT

Additional Product Accessories

Optional Upgrades:
Free Upgrade to Preloaded Desktop PC w/ 24" Monitor - ($500 Value)

Recertification Protocol
Technical Specification Sheet

Biotek Synergy HT Microplate Reader

Detector Modes
Absorbance, Fluorescence, and Luminescence

Optics Accuracy
± 2 nm

Optics Range
200 to 999 nm

  • Single-channel microplate reader for absorbance, fluorescence, and luminescence.
  • Computer-controlled using BioTek's Gen5 software for all operations and data analysis.
  • Robot accessible and compatible with BioTek's BioStack Microplate Stacker.
  • Utilizes tungsten quartz halogen lamp and interference filters for fluorescence measurements.
  • Luminescence measured through a low-noise PMT detector with an empty filter position.
  • Absorbance measurements made using a xenon flash lamp and monochromator.
  • 4-Zone temperature control (4°C to 50°C) for assay flexibility.
  • Supports 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. Includes internal plate shaking for reagent mixing.
The Biotek Synergy HT is a single-channel absorbance, fluorescence, and luminescence microplate reader. It is computer-controlled using BioTek’s Gen5 or KC4 PC software for all operations including data reduction and analysis. The Synergy HT is robot accessible and compatible with BioTek’s BioStack Microplate Stacker. When making fluorescence determinations, the Synergy HT uses a tungsten quartz halogen lamp with interference filters for wavelength specificity in conjunction with a photomultiplier (PMT) tube detector. Luminescence is measured by the low-noise PMT detector through an empty filter position in the Emission filter wheel. Absorbance measurements are made by switching to a xenon flash lamp and a monochromator for wavelength selection. The Synergy HT has a 4-Zone temperature control from 4°C over ambient to 50°C and supports the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. Internal plate shaking is supported to ensure that reagents are properly mixed prior to reading.

The Biotek Synergy HT Microplate Reader includes:
  • > Windows 10 Data Station with Pre-Loaded Gen5 3.11 PC Software
  • > ABS, FLD, and LUM Calibration Report
  • > Operator's Manual
  • > Interface Cable
  • > Full 180 Day Part and Labor Warranty

Biotek Synergy HT Multimode Microplate Reader Specifications

Microplate Capacity

Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates

Max Plate Height:

Luminescence/Fluorescence modes: plates up to 1.25” (31.75 mm) high
Absorbance mode: plates up to 0.8” (20.30 mm) high

Dimensions (D x W x H)

16 x 15 x 10 in

40.6 cm x 38 cm x 25.4 cm


38 lb. (17 kg)


Operational temperature 18º to 40°C

Light Source

Absorbance: Xe flash light source

Fluorescence: Tungsten quartz halogen


10% to 85% relative humidity (non-condensing)

Power Consumption

100 VA max, 130 VA max with injectors


Temperature control range from 4º over ambient to 50ºC

Temperature variation ± 0.50°C across the plate @ 37°C (250 µL per well with the plate sealed)

Plate Shaking

Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user

Read Modes

Normal, Rapid and Sweep

Absorbance Measurement Range

0.000 to 4.000 OD

Absorbance Accuracy

2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates

Absorbance Linearity

2.000 to 2.500 OD ± 3.0% Normal and Rapid modes, 96- and 384-well plates

Absorbance Repeatability

0.000 to 2.000 OD ± 1.0% ± 0.005 OD Normal and Rapid modes, 96- and 384-well plates

Fluorescence Read Timing

Excitation Filter: 485/20 nm

Emission Filter: 528/20 nm

96-well read: 89 sec

384-well read: 275 sec

Fluorescence Sensitivity

5mm probe: 10 pg/ml solution of Sodium Fluorescein in PBS

3mm probe: 20 pg/ml solution of Sodium Fluorescein in PBS

1.5mm probe: 40 pg/ml solution of Sodium Fluorescein in PBS

Time Resolved Fluorescence

Delay 0 or 20 µs to 16000 µs

Integration interval 20 to 16000 µs

Times adjustable in 10 µs increments

Computer Specifications


Intel I5-4300U CPU


16.0 GB



Hard drive

240GB Solid State Drive

Operating System

Windows 10 64-Bit

BioTek Synergy HT Manual

Click Here to View the Technical Specs for the Synergy HT

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Average Rating: Average Rating: 5 of 5 5 of 5 Total Reviews: 1 Write a review »

  0 of 0 people found the following review helpful:
5 of 5 Fast and Quiet December 29, 2023
Reviewer: Isaac Duncan  
great tool for high-throughput screening. It delivered accurate readings every time. Software worked perfectly and was very intuitive. Would recommend to a colleague

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