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BioTek 405LSUVS Microplate Washer
The BioTek 405LSUVS Microplate Washer is known for its superior performance and reliability. The 405 LS features a simple user interface for implementing wash, dispense, aspirate, and maintenance protocols with the onboard software. The 405 LS is ideal for many standard plate washing protocols of 96- and 384-well microplates, but it can also automate more complex operations like magnetic and polystyrene bead assays and ELISAs. The patented dual-action manifold makes cell-based assays easy to process while providing optimal conditions for washing fragile cell layers with the angles dispense tubes, extensive aspirate and dispense rates, and plate position controls.Programming is made easy through simple keypad interface with independent control of plate position during dispense and aspiration cycles, as well as fully programmable fluid volumes and flow rates, shaking, and soaking. Easy maintenance with a selection of pre-programmed routines for problem-free operation and run protection via fluid detection, fluid flow, and vacuum sensors. This model features Dual Action to wash 96- and 384-well plates, Buffering Switching Valve that support up to four reagents, and Ultrasonic Advantage feature, a built-in ultrasonic cleaner.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The BioTek 405LSUVS Microplate Washer includes:
- > Waste & Buffer Bottles
- > Vacuum Pump
- > Power Cord
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
BioTek 405LSUVS Microplate Washer Specifications
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General
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Microplate types
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96- and 384-well
Low profile and standard height
Solid and filter bottom (option)
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Onboard software
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Create, edit or run multiple protocols
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Software (computer control)
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LHC2 pc software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
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Separation
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Biomagnetic separation, vacuum filtration (optional)
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Shaking
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Programmable up to 60 minutes
Slow, medium, fast or variable
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Soaking
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Programmable up to 60 minutes
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Ultrasonic Advantage™
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Yes (option)
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Verify™ clog detection
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Yes (option)
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Automation
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BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
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Washing
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Manifold types
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96-well washing: 96-tube manifold - straight dispense tubes
96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense
tubes
384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense
tubes
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Volume range
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25 to 3,000 µL/well, in 1 µL increments
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Wash cycles
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1 - 250
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Buffer/reagent selection
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Auto switching (internal) for up to 4 buffers (option)
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Supply bottle
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4 L or 10 L (optional)
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Dispense precision
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<3% CV: 300 µL/well (96-well washing)
<4% CV: 80 µL/well (384-well washing)
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Residual volume
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<2 µL/well (96- & 384-well plates)
96-tube manifold for 96 wells; 192-tube for 384 wells
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Wash speed
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96-wells, 300 µL/well, 3 cycles;
<30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
384-wells, 400 µL/well, 1 cycle: <20 seconds
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Flow rates
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High flow to low flow
Optimized rates for cell assays
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Sterilization
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Chemical
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Vacuum filtration
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Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg
Vacuum filtration time range: 5 to 999 seconds
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Physical Characteristics
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Power
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100 - 240 Volts AC. 50/60 Hz
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Dimensions
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14" W x 17" D x 10" H
(35.6 x 43.2 x 25.4 cm)
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Weight
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With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
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Operator's Manual
 Click Here to View the Technical Specs for the 405LSUVS
Click Here for an Overview Comparision of Biotek ELX405 Microplate Washer Models
- Install new waste and buffer bottles to ensure proper fluid containment and system functionality.
- Integrate a new pump into the system, verifying compatibility and secure connections.
- Verify that the power supply and PCB output the correct voltages for reliable component operation.
- Conduct a self-test on the microplate washer, evaluating all operational parameters.
- Inspect the X-axis motor belt for signs of wear, such as tears, frays, or breakages, replacing if necessary.
- Examine dispense and aspirate tubes for any damage that could affect fluid flow accuracy.
- Confirm internal pump functionality, replacing seals as needed to maintain optimal performance.
- Inspect all internal wiring for frays, tears, or breakages, addressing any faults for electrical safety.
- Verify functionality of all internal and external sensors, ensuring accurate readings across the unit.
- Disinfect the unit using an appropriate disinfection cycle to ensure sterility and safety.
- Check that residual aspiration levels are within the manufacturer’s specified limits for accuracy.
- Conduct a final cosmetic inspection to verify that all external surfaces meet appearance standards.
Technician Inspection and Recertification Checklist: Microplate Washer
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Completed? |
Remarks? |
| Install new waste and buffer bottles | Completed | |
| Integrate new pump into system, verifying secure connections | Completed | |
| Verify power supply and PCB output correct voltages | Completed | |
| Conduct microplate washer self-test and evaluate results | Completed | |
| Inspect X-axis motor belt for wear, replacing if necessary | Completed | |
| Examine dispense and aspirate tubes for any damage | Completed | |
| Confirm internal pump functionality, replace seals as needed | Completed | |
| Inspect internal wiring for frays, tears, or breakages | Completed | |
| Verify functionality of all internal and external sensors | Completed | |
| Disinfect unit with appropriate disinfection cycle | Completed | |
| Check residual aspiration levels meet specifications | Completed | |
| Conduct cosmetic inspection for appearance standards | Completed | |
To View More of Our Recertification Protocol Click Here
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