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BD FACSCAlibur 2 Laser, 4 Color Flow Cytometer
BD FACSCAlibur 2 Laser, 4 Color Flow Cytometer
Click Here to View the Technical Specs for the FACSCalibur
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Our Price: $15,365.00

Condition: Fully Refurbished and Tested to Perform at Manufacturer Specifications
Warranty: 90-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 5 to 10 Business Days

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Quantity in Stock: Unfortunately

Product Code: BF-FACS

Refurbishment Protocol
Technical Specification Sheet (NEW)

BD FACSCalibur 2 Laser, 4 Color Flow Cytometer

The Becton Dickinson FACSCalibur Flow Cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. The BD FACSCalibur is a fully integrated multiparameter system that has the performance and sensitivity to ensure objective and reproducible results through the use of dual-laser technology, interbeam compensation, and an alignment-free optical design. Efficient collection optics, coupled to the flow cell with an optical gel, permit the use of a low-powered, air-cooled argon laser and a red diode laser to ensure the high sensitivity required for analysis of low-density antigens.

The BD High Throughput Sampler (HTS) option provides flexible, easy-to-use automation. The HTS option allows users to rapidly acquire samples with the BD FACSCalibur flow cytometer in a microtiter plate. The HTS option supports 96 U-, V-, and flat-bottom plates as well as 384-well microtiter plates.

The BD FACSCaliber system is best used for routine cell analysis, assay development, verification, and identification of cellular populations of interest. Streamlined software analysis applications include enumeration of lymphocyte subsets, stem cells, residual white blood cells, reticulocytes, DNA analysis, immune function studies, bead based immunoassays, and multiplexed analysis of signal transduction and phosphorylation targets.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Becton Dickinson Facscalibur Flow Cytometer Includes:
- Mac G5 Computer w/ CellQuest Pro Software
- Software Dongle
- 2 Lasers, 4 Colors
- Flatscreen Monitor
- Calibration Report
- All Power Cords, Tubing, Hoses
- Full 90 Day Parts and Labor Warranty

BD FACSCalibur 2 Laser, 4 Color Flow Cytometer Specifications

Fluorescence Sensitivity

Estimated detection limit is 750 molecules of equivalent soluble fluorescein

Fluorescence Resolution

Coefficient of variation in FL2-Area of <3%, full peak for propidium iodide-stained chicken erythrocyte nuclei

Forward and Side Scatter Sensitivity

Sensitivity enables the separation of fixed platelets from noise

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes

Optical Platform

Fixed optical assembly


15 milliwatt 488 nm, air-cooled Argon-ion laser; life expectancy >5,000 hours Optional second laser: nominally 635 nm

Beam Geometry

Prismatic expander and achromatic spherical lens provide 22 µm x 66 µm elliptical beam for argon ion laser, and nominally 15 µm x 61 µm elliptical beam for red diode laser

Optical Coupling

Quartz cuvette is coupled to emission lens by refractive index matching optical gel for optimum collection efficiency

Background Rejection

Obscuration blade and slit minimize unwanted laser radiation at the detector

Forward Scatter Detector and Filter

High performance solid state silicon detector with 488 nm band pass filter for clear signal detection, and red diode laser signal rejection

Side Scatter Detector

High performance photomultiplier using Brewster angle beam splitter in the emission optical train

Fluorescence Detectors and Filters

Four high performance, high dynamic range photomultipliers with band pass filters: 530 nm (FITC), 585 nm (PE/PI), 661 nm (APC), and >650 nm (PerCP) with base unit, >670 nm (PerCP) with FL4 option

BD FACSCalibur 2 Cytometer Manual

Click Here to View all the Technical Specs for the FACSCalibur

Why Buy From Marshall Scientific?

Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.

All Lab Equipment is Fully Refurbished and Tested

Most Pieces Ship within 2 to 5 Business Days

All Products Come with a Standard Warranty

Trained Customer and Technical Support

Industry Leading Overall Customer Satisfaction

Marshall Scientific's Certified Refurbishment Protocol


Our technicians decontaminate and clean all equipment upon arrival and upon purchase to create a neutral environment for lab use.

Preliminary testing is conducted to identify potential product defects, upon completion, we replace all faulty and/or under-performing parts with original manufacturer parts that perform up to specification.

Any parts known to fail or malfunction from excessive use are removed and replaced to insure the extended lifetime of your equipment.

Any necessary preventative maintenance is performed alongside repairs, so your purchase will be ready to go as soon as it arrives.

All electronics and boards are tested for proper function and response by our industry trained technicians. Any electronic deficiencies detected are immediately repaired.

Final testing cycles ensure your instrument performs fully at manufacturer specifications, guaranteeing you receive a properly working product ready for use.

Once we finish our rigorous testing and decontamination procedures, your order will be packaged to best protect it during shipment and will include delivery insurance.

Stanford University
We love shopping at Marshall Scientific! Great products, prices, and performance. Marshall Scientific provides 'tried and true' equipment at a fraction of original purchase price. I always shop here first!
Meg W. / Stanford University
Easy to find what you need with clear specs, website ordering was simple, equipment arrived quickly, very well packaged and working as expected! I couldn't ask for more, I'll definitely use them again!
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University of Maryland
They do a very high quality job worth every penny they charge. Customer service is fantastic, they can always be reached by phone or email, and the staff are very knowledgeable and ready to help.
Igor P. / University of Maryland
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Average Rating: Average Rating: 5 of 5 5 of 5 Total Reviews: 1 Write a review »

  2 of 2 people found the following review helpful:
5 of 5 Awesome December 8, 2018
Reviewer: Roy Bryan from Forest Park, OK  
Fast shipping, product just as described, great service. Simple as that.

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