BD Biosciences LSR II Flow Cytometer

The BD Biosciences LSR II is a multi-laser flow cytometer that utilizes fixed-alignment lasers that transmit light reflected by mirrors through a flow cell to user-defined octagon and trigon arrays. These fluorescence signals are collected and translated to electronic signals then converted to digital data. The BD LSR II utilizes innovative optics and digital electronics to provide highly flexible yet powerful performance with a greater sensitivity, which yields more information from each sample.

The BD LSR II can be configured with one to four fixed-aligned, air-cooled lasers; this configuration features three lasers for eleven color excitation. These solid state lasers consist of the Coherent Sapphire, Coherent VioFlame Plus, and Lightwave Xcyte, all designed to consume less power, produce less heat, and have a lower overall lifetime cost compared to gas-filled lasers. The Coherent Sapphire (blue, 488 nm) produces no plasma glow, lowering noise during excitation. The Coherent VioFlame Plus (violet, 405 nm) excites Cascade Blue, Pacific Blue, and Alexa Fluor 405, along with BD Clontech Living Colors GFP, YFP, dsRed, and CFP. The Lightwave Xcyte (UV, 355 nm) allows routine experiments to be performed on samples with UV dyes, such as DAPI, Hoechst, and Indo-1.

The collection optics includes patented octagon and trigon optical arrays, which increase sensitivity and flexibility for more sample information. The 488-nm octagon array can collect up to seven colors along with side scatter, while the 633-nm, 405-nm, and 355-nm trigon arrays can detect up to three colors. Acting upon the principle that the reflectance of light is more efficient than the transmittance, more colors can be detected with minimum light loss.

The BD LSR II utilizes BD FacsDiva software, which provides a means for experiment design, instrument control, and data analysis. FacsDiva allows for improved workflow, featuring reusable acquisition templates, an auto-spillover matrix for automated compensation, offline compensation, and Biexponential data display for better visualization of digital data. All experiment data, including instrument settings, plots, compensation matrices, and statistics, is stored in a database for reuse and reanalysis.

• Multilaser multiparameter analysis cytometer
• Three lasers, 11 colors
• Automatic compensation (compensation controls are still needed)
• 355 nm (UV): BUV737, BUV395, DAPI
• 405 nm (Violet): CFP, Alexa Fluor 430, Pacific Blue, Marina Blue, Alexa Fluor 405
• 488 nm (Blue): FITC, PE, PerCP, Pe-Cy5 (aka CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas Red
• 633 nm (Red): APC, APC-Cy7, Alexa Fluor 647, Alexa 680
• Availability of UV gives a capability to perform Ca 2+ flux experiments.
• Unlike a FACSCalibur, it is possible to use PE-Cy5 and APC together.

BD Biosciences LSR II Flow Cytometer Specifications

All of our equipment is fully tested to perform at factory specifications before being shipped.

Click Here to View the Technical Specs for the LSR II