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Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block
Block Configuration
96-Well (10 to 100 uL reactions)
Maximum Ramp Rate
6.5°C/second
Temperature Uniformity
± 0.4°C
- Six decoupled optical channels with 21 filter combinations support precise multiplex detection
- VeriFlex technology enables independent temperature control across six zones for flexible assay development
- Bright white LED ensures consistent excitation for reliable fluorescence detection
- Optical scanning system delivers accurate, high-sensitivity results across diverse fluorophores
- Touchscreen interface with intuitive navigation simplifies setup and run monitoring
- Cloud connectivity enables secure remote data access, storage, and collaboration
- Preprogrammed protocol templates streamline workflow for common real-time PCR applications
The Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block is designed to deliver high-performance results for a wide range of molecular biology applications. With six independently controlled temperature zones through VeriFlex technology, it allows for precise thermal cycling and flexible assay development. The system supports up to six-target multiplexing with 21 filter combinations and decoupled optical channels—making it ideal for complex gene expression studies and SNP genotyping.
A bright white LED provides consistent excitation, while whole-plate imaging captures accurate and reproducible data without mechanical scanning. Its intuitive touchscreen interface enables real-time monitoring and simplified program setup, and the system integrates with a cloud-based platform for secure data access and collaboration. With fast ramp rates, preoptimized protocol templates, and 21 CFR Part 11–ready software features including auditing and e-signatures, the QuantStudio 5 streamlines workflows without sacrificing precision or traceability. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block includes:
- > 0.2ml 96-Well Block
- > Background Calibration Report
- > ROI Calibration Report
- > Uniformity Calibration Report
- > Windows 11 Professional 64-Bit Data Station
- > QuantStudio Design & Analysis Desktop Software Installed
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 96-Well Sample Block Specifications
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Block Configuration
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96-Well (10 to 100 uL reactions)
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Maximum Ramp Rate
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6.5°C/second
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Average Sample Ramp Rate
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3.66°C/second
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Temperature Uniformity
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± 0.4°C
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Run Time
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Less than 30 minutes
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Heating and Cooling Method
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Peltier
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Excitation (Light Source)
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Bright white LED
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Filters or Colors
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6 decoupled filters
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Excitation and Detection Range
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450 to 680 nm and 500 to 730 nm
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Multiplexing
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Up to 6 targets
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Dynamic Range
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10 logarithmic units
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Sensitivity (Resolution)
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Detect differences as small as 1.5-fold in target quantities in a singleplex reaction
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Data Acquisition
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Whole-plate imaging
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Onboard Memory
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10 GB, which translates to approximately 2,000 to 5,000 run files
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Dye Compatibility
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FAM™/SYBR™ Green, VIC™/JOE™/HEX™/TET™, ABY™/NED™/TAMRA™/Cy®3, JUN™, ROX™/Texas Red™, Mustang Purple™, Cy®5/LIZ™, Cy®5.5
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Dimensions (W x D x H)
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10.6 x 19.7 x 15.7 in. (27 x 50 x 40 cm)
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Weight
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57.3 lb. (26 kg)
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Electrical Requirements
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100-240V, 50/60Hz
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Computer Specifications
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Processor
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Intel Core I5-8350U CPU
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RAM
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16.0 GB
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Hard Drive
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512GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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