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Applied Biosystems 7500 Real-Time PCR System
Block Configuration
96-Well
Maximum Ramp Rate
2.5°C/second
Temperature Uniformity
± 0.5°C
- Highly suitable for a variety of applications including gene expression analysis and DNA quantification
- Integrated real-time PCR solutions with optimized reagents and customizable assays
- Capable of delivering results in less than 2 hours
- Optical system includes CCD camera and halogen lamp with five excitation and emission filters
- Supports multiplexing of up to five targets per well
- Features a dynamic range of nine logs
- Sensitivity to detect one copy of a template in 50 uL reactions with 99.7% confidence
The Applied Biosystems 7500 Real-Time PCR System is designed for advanced PCR applications, ideal for genetic analysis and molecular diagnostics. This system features a robust 96-well block, supporting reaction volumes from 20 to 100 uL, optimized for precise and efficient thermal cycling. Key characteristics of the system include a temperature range of 4°C to 100°C, ensuring broad flexibility for various assays. The temperature uniformity is maintained at ± 0.5°C, achieved 30 seconds after the cycle starts, with an accuracy of ± 0.25°C, measured three minutes after the cycle begins. This precision is critical for consistent results across all wells.
The system boasts a maximum ramp rate of 2.5°C per second, enabling completion of runs in less than two hours. Its optical setup includes a CCD camera and a halogen lamp for excitation, accompanied by five excitation and emission filters that allow for sensitive multiplex detection of up to five targets per well. With a dynamic range spanning nine logs, the 7500 system can detect a single copy of a template in a 50 uL reaction volume with 99.7% confidence. This sensitivity makes it an indispensable tool for laboratories requiring reliable, high-throughput PCR capabilities. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Applied Biosystems 7500 Real-Time PCR System includes:
- > 96-Well Block
- > New Halogen Lamp
- > Background Calibration Report
- > ROI Calibration Report
- > Optical Calibration Report
- > Windows 11 Professional 64-Bit Data Station
- > Applied Biosystems 7500 Software Installed
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Applied Biosystems 7500 Real-Time PCR System Specifications
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Block Configuration
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96-Well (20 to 100 uL reactions)
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Temperature Range
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4°C to 100°C
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Temperature Uniformity
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± 0.5°C (30 seconds after clock start)
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Temperature Accuracy
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± 0.25°C of setpoint/display temperature (measured at 3 minutes after clock start)
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Maximum Ramp Rate
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2.5°C/second
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Run Time
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< 2 hours
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Optical System
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CCD camera with halogen lamp excitation; five-exciation and five-emission filters
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Dynamic Range
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9 logs of linear dynamic range
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Sensitivity
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Detection of 1 copy of template in 50 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
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Multiplexing
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Up to 5 targets per well
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Dye Compatibility
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FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual)
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Passive Reference Dyes
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ROX dye or any calibrated dye
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Dimensions (W x D x H)
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13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
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Weight
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75 lb. (34 kg)
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Electrical Requirements
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100-240V, 50/60Hz, 1080W
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Computer Specifications
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Processor
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Intel Core I5-8350U CPU
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RAM
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16.0 GB
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Hard Drive
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512GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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