Applied Biosystems 7500 Fast Dx Real-Time PCR System

The Applied Biosystems 7500 Fast Dx Real-Time PCR System, FDA-cleared for In Vitro Diagnostic (IVD) use, delivers reliable, high-throughput detection of nucleic acid targets with five-color fluorescence capability. This system, built on over ten years of real-time PCR innovation, is tailored for high-throughput environments requiring both speed and accuracy. It features a unique 96-well Fast block that accommodates reaction volumes from 10 to 30 uL, but is optimized for 10 uL, ensuring consistent thermal uniformity across all samples.

The system's rapid ramp rates accelerate the processing times to deliver results in less than 40 minutes without compromising the extension times or the quality of the assay. This efficiency is matched by the system’s robust temperature controls, maintaining a uniformity of ± 1°C and an accuracy of ± 0.5°C, which are crucial for precise thermal cycling. Additionally, the optical system uses a CCD camera coupled with a halogen lamp for excitation, along with five excitation and emission filters, enabling sensitive multiplex detection of up to five targets per well.

With a dynamic range extending over nine logs, the 7500 Fast Dx can detect as little as one copy of a template in a 20 uL reaction with 99.7% confidence. This system is not only a testament to Applied Biosystems' legacy of quality and innovation but also an essential tool for modern laboratories focused on genetic analysis and molecular diagnostics. The SDS v1.4.1 software on the 7500 Fast Dx has Security, Auditing and E-Signature features, which support regulated environments. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions. This instrument has been discontinued by the manufacturer but remains suitable for validated IVD applications and laboratory use.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Applied Biosystems 7500 Fast Dx Real-Time PCR System Specifications

Block Configuration	96-Well Fast (10 to 30 uL reactions, optimized for 10 uL reactions)
Sample Format	Fast 96-Well plates (100 uL) or 8-tube strips (100 uL)
Temperature Range	4°C to 99.9°C
Temperature Uniformity	± 1°C
Temperature Accuracy	± 0.5°C
Sample Ramp Rate	Fast mode: 3.5°C/sec Standard mode: 1.6°C/sec
Peak Block Heating Rate	5.5°C/second
Run Time	< 40 minutes
Optical System	CCD camera with halogen lamp excitation; five-exciation and five-emission filters
Dynamic Range	9 logs of linear dynamic range
Sensitivity	Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence
Multiplexing	Up to 5 targets per well
Dye Compatibility	FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual)
Passive Reference Dyes	ROX dye or any calibrated dye (use of a passive reference dye is optional)
Dimensions (W x D x H)	13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm)
Weight	75 lb. (34 kg)
Electrical Requirements	100-240V, 50/60Hz, 1080W