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Agilent 6545A Q-TOF Mass Spectrometer w/ 1260 Infinity I LC System
Mass Range
m/z 100 to 10,000
Mass Accuracy
Less than 0.8 ppm RMS
Spectral Acquisition Rate
50 spectra/second
- Raises the standard for mid-range Q-TOF instruments with significantly higher resolving power and sensitivity
- Up to five times increased sensitivity over previous models, ideal for small molecule analysis
- Features a fast and powerful new autotune capability to optimize performance effortlessly
- Incorporates robust ion optics and beam shaping technology to extend maintenance intervals and enhance consistency
- Equipped with new high voltage pulsers and power supplies for enhanced mass resolution and accuracy up to 0.8 ppm
- Includes the advanced MassHunter software suite with powerful data-mining tools for faster, more accurate analyses
- Offers a wide dynamic range of up to five decades for comprehensive sample analysis
- Quick switching between positive and negative ion modes, optimizing time efficiency in analytical workflows
- Enhanced electronics and software algorithms support exceedingly high data acquisition rates
- Automated slicer assembly and continuous refinement in ion optics design combine to deliver robust performance
- New-generation detector offers significantly improved longevity, maintaining high sensitivity and a wide dynamic range
The Agilent 6545A Q-TOF Mass Spectrometer w/ 1260 Infinity I LC System represents a significant advancement in mid-range quadrupole time-of-flight mass spectrometry, offering an unparalleled combination of resolving power and sensitivity, which is estimated to be up to five times greater than previous models. This instrument is expertly designed for diverse applications including pharmaceutical research, food safety, environmental testing, metabolomics, lipidomics, and other small molecule analyses. Featuring a newly developed fast autotune technology, the 6545A enhances sensitivity specifically for small molecules, improving detection limits and maintaining the integrity of fragile compounds. This innovative autotune also streamlines the optimization process, making it faster and more efficient, like an autofocus system in cameras but for mass spectrometry.
The system's robust ion optics are engineered to provide greater durability and consistency in results, extending the intervals between required maintenance. The introduction of new ion beam shaping optics further stabilizes the ion flow, enhancing analytical consistency and prolonging instrument uptime. Highly sophisticated new-generation electronics and power supplies increase the mass resolution performance and enhance mass accuracy to an impressive 0.8 ppm. These features ensure high precision in mass determination, crucial for complex sample analyses.
Agilent has also integrated cutting-edge enhancements in the MassHunter software suite with this system. These advancements include powerful data-mining capabilities that allow users to rapidly and accurately process and interpret analytical data, not just identifying peaks and data points, but also providing comprehensive answers based on the chemical data. The 6545A Q-TOF supports an extensive dynamic range of up to five decades, accommodating a wide variety of sample concentrations within a single analysis. It covers a broad mass range from m/z 50 to 10,000, allowing for flexible and extensive molecular weight analysis. The system's spectral acquisition rate is notably high, capturing 50 spectra per second in MS mode and 30 MS/MS spectra per second, which facilitates high-throughput screening and detailed product ion analysis in MS/MS mode.
Transitioning between positive and negative ion modes is seamless and swift, with a cycle completion time of just 1.5 seconds. This feature is particularly valuable in labs performing comprehensive compound analyses, as it minimizes downtime and maximizes productivity. Overall, the Agilent 6545A Q-TOF is designed to boost laboratory efficiency and analytical confidence, providing robust, high-productivity, and high-sensitivity performance across a variety of demanding applications. It sets a new standard for Q-TOF instruments, making it an essential tool for scientists seeking to push the boundaries of what is possible in their research.
This Agilent LC/MS system was recertified and calibrated by a factory trained engineer and is ready to ship on demand. This system comes with everything needed for easy installation and setup. All of our equipment is fully tested to perform at factory specifications before being shipped.
The Agilent 6545A Q-TOF Mass Spectrometer w/ 1260 Infinity I LC System includes:
- > Agilent 6545A Q-TOF Mass Spectrometer
- > Agilent MS40+ Rotary Vane Vacuum Pump
- > Agilent G1958B Jet Stream Electrospray Ionization Source
- > G1312B Binary Pump
- > G1367E Autosampler
- > G1330B ALS Thermostat
- > G1316A TCC
- > G4225A Degasser
- > G1314F VWD Detector
- > Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
- > Agilent MassHunter B.09.01 Acquisition and Data Analysis Software Installed
- > Agilent MassHunter BioConfirm Software Installed
- > All Power Cords
- > Can/Crossover Cables
- > Agilent Lan Interface Card Built-In
- > Solvent Tubing
- > Capillaries
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report & Mass Spec Tuning Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1260 Infinity I LC System.
Agilent 6545A Q-TOF Mass Spectrometer w/ 1260 Infinity I LC System Specifications
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Sensitivity, MS Mode, Electrospray On-Column, 400 uL/min Flow Rate
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500:1 RMS (1 pg LC/MS injection of reserpine. Signal-to-noise for the reserpine (M+H)+ at m/z 609.2807 while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode)
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Sensitivity, MS/MS Mode, Electrospray On-Column, 400 uL/min Flow Rate
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1,500:1 RMS (1 pg LC/MS injection of reserpine. Signal-to-noise for most intense product ions (174, 195, 397, and 448 m/z) while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode)
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Mass Resolving Power
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45,000 FWHM at m/z 2,722 (measured after automatic tuning procedure)
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Mass Accuracy - MS Mode, Electrospray On-Column, 400 uL/min
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Less than 0.8 ppm RMS as measured from 10 repeat injections (measured at the (M+H)+ ion of reserpine (m/z 609.2807) using an internal mass reference)
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Mass Accuracy - MS/MS Mode, Electrospray On-Column, 400 uL/min
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Less than 2 ppm RMS on m/z 397 as measured from 10 repeat injections (product ion m/z 397 for reserpine)
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Dynamic Range
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Up to 5 decades (intrascan dynamic range on co-eluting components)
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Mass Range
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m/z 100 to 10,000 for extended mass range
m/z 50 to 1,700 or 50 to 3,200 for both high resolution and extended dynamic range modes
Quadrupole up to m/z 4,000
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Spectral Acquisition Rate, MS Mode
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50 spectra/second (m/z 50 to 1,700 in MS mode while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode)
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Spectral Acquisition Rate, MS/MS Mode
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30 MS/MS spectra/second (m/z 50 to 1,700 in MS/MS mode while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode)
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Positive to Negative Switching
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1.5 seconds (complete cycle switching from positive to negative and positive modes allow for stabilization time)
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Dimensions (H x D x W)
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76 x 30 x 48 in. (193 x 76.2 x 121.9 cm)
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Weight
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375 lb. (170 kg)
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Electrical Requirements
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200-240VAC, 50/60Hz
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Click Here to View Specifications for the Agilent 1260 Infinity I LC System
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VWD Detector
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Specification
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Detection Type
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Double-beam photometer
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Light Source
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Deuterium lamp
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Wavelength Range
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190 - 600 nm
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Short Term Noise (ASTM) Single and Multi-Wavelength
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< ± 0.5 × 10^-5 AU at 254 nm
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Drift
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3 × 10^-4 AU/hr at 254 nm
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Linearity
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> 2.0 AU (5%) upper limit
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Band Width
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6.5 nm typical
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Maximum Data Rate
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55 Hz
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Signal Data Rate
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Up to 80 Hz
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Spectra Data Rate
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Up to 80 Hz
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Flow Cell
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Standard: 14 µL volume, 10 mm cell path length and 40 bar (580 psi) pressure maximum
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Binary Pump
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Specification
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Hydraulic System
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Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
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Setable Flow Range
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0.001 – 5 ml/min, in 0.001 ml/min increments
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Flow Range
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0.5 - 5.0 mL/min
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Flow Precision
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≤ 0.07% RSD or ≤ 0.02 min SD, whatever is greater
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Flow Accuracy
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± 1% or 10 µl/min, what ever is greater
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Pressure Operating Range
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Operating range 0 to 600 bar (0 – 7800 psi) up to 5 ml/min
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Pressure Pulsation
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< 2 % amplitude (typically < 1.3 %), or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
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Compressibility Compensation
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User-selectable, based on mobile phase compressibility
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Recommended pH Range
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1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless stee
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Gradient Formation
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High-pressure binary mixing
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Delay Volume
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600 to 800 µl, dependent on back pressure
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Composition Range
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0 to 100% (1 to 99% or 5 µl/min per channel, whatever is greater is recommended)
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Composition Precision
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< 0.15% RSD or < 0.04 min SD whatever is greater
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Composition Accuracy
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± 0.35% absolute at 2 mL/min, at 10 MPa (100 bar)
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Autosampler
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Specification
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Injection Range
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0.1 - 100 µl in 0.1 µl increments
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Precision
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< 0.25% from 5 to 40 uL
< 0.5% from 2 to 5 uL
< 0.7% from 1 to 2 uL
< 1.5% from 0.5 to 1 uL
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Pressure Range
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Operating range 0 - 60 MPa (0 - 600 bar, 0 - 8850 psi)
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Sample Viscosity Range
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0.2 to 5 cp
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Sample Capacity
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2 x well plates (MTP) + 10 x 2 ml vials
108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 ml vials
30 x 6 ml vials in 2 x 15 vial plate
100 micro vial tray plus 10 additional 2 ml vials
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Injection Cycle Time
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Typically < 21 seconds using default conditions and injection volume of 5 uL
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Carryover
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Typically < 0.004%
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ALS Thermostat
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Specification
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Temperature Range
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4°C to 40°C in 1°C increments
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Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50%
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3°C to +8°C at a setpoint of 4°C
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Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60%
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3°C to +9°C at a setpoint of 4°C
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Column Compartment
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Specification
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Temperature Range
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Ambient -10°C to 80°C at flow rates up to 5 mL/min
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Temperature Stability
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± 0.15 °C
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Temperature Accuracy
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± 0.8°C (± 0.5°C with calibration)
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Column Capacity
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Three 30 cm
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Warm-Up/Cool-Down Time
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5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
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Dead Volume
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3 µl left heat exchanger
6 µl right heat exchanger
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Degasser
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Specification
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Number of Solvent Channels
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4
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Maximum Flow Rate
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0 - 5 mL/min per channel
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Internal Volume Per Channel
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Typically 1 ml per channel
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Materials in Contact with Solvent
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PTFE, PEEK
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pH Range
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1 - 14
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Computer Specifications
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Model
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Dell Optiplex 7010 Desktop PC
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Processor
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Intel Core i5-6500 Processor 3.2GHz
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RAM
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16GB
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Hard Drive
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250GB Internal SSD
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Operating System
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Windows 10 Professional, 64bit
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