Agilent 1290 Infinity I HPLC G4226A Autosampler

The Agilent 1290 Infinity I HPLC G4226A Autosampler is engineered for high-speed, high-pressure liquid chromatography applications that require reliable sample handling and minimal carryover. Designed to support modern narrow-bore column technologies, the autosampler delivers robust performance under demanding conditions while maintaining efficient sample throughput for advanced analytical workflows.

The G4226A uses an X-Z-theta robotic transport mechanism driven by stepper motors and monitored by optical encoders to ensure precise positioning of the sampling arm for both vials and well plates. Samples are drawn into the needle by an integrated metering device and flushed directly onto the column through a controlled injection sequence. A vial and plate pusher mechanism stabilizes the sample container during needle withdrawal, supporting reliable operation when septa are used.

To reduce carryover, the autosampler incorporates a flow-through design that flushes the complete flow path with mobile phase after injection, along with a dedicated needle flush station that rinses the exterior of the needle using a peristaltic pump. A high-speed injection valve directs flow either through the autosampler or directly to the column, ensuring efficient sample transfer and clean system conditions between runs. Together, these design elements make the Agilent 1290 Infinity I G4226A Autosampler a dependable solution for high-throughput, low-carryover LC analysis in demanding laboratory environments.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Agilent 1290 Infinity I HPLC G4226A Autosampler Specifications

Pressure	Up to 1200 bar
GLP Features	Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits and feedback messages. Electronic records of maintenance and errors
Communications	Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, optional four external contact closures and BCD vial number output
Safety Features	Leak detection and safe leak handling, leak output signal for shutdown of pumping system, low voltages in maintenance areas, error detection and display
Injection Range	0.1 - 20 uL in 0.1 uL increments
Precision	Typically < 0.25% RSD of peak areas from 5 to 20 uL Typically < 0.5% RSD of peak areas from 2 to 5 uL
Carryover	Typically < 0.004%
Sample Viscosity Range	0.2 - 5 cp
Metering Devices	Metering pump in high pressure flow path
Sample Capacity	2 x well plates (MTP) + 10 x 2 mL vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate 100 Micro vial tray, plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2 mL) in 2 x 27 Eppendorf tube plate
Injection Cycle Time	Typically < 21 s with draw speed of 100 uL/min and ejection speed of 100 uL/min with injection volume of 5 uL
Ambient Operating Temperature	4 to 55°C
Dimensions (W x D x H)	13.5 x 17 x 8 in. (34.5 x 44 x 20 cm)
Weight	34.2 lb. (15.5 kg)
Electrical Requirements	100-240V, 50/60Hz, 200W