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Agilent 1260 Infinity II LC System w/ VWD
Agilent 1260 Infinity II LC System w/ VWD

Our Price: $43,995.00

Price with Selected Options: $43,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report

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Product Code: AG-1260HPLCVWDII

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Recertification Protocol
Technical Specification Sheet

Agilent 1260 Infinity II LC System w/ VWD

The Agilent 1260 Infinity II LC System w/ VWD provides a liquid chromatograph offering ultra fast and high resolution separation capability for consistently accurate results. The 1260 Infinity II LC System delivers several ease-of-use features, making it a standard system for routine analysis. With an operating pressure of up to 600 bar the 1260 Infinity II Quaternary Pump is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID columns over the flow rate range (up to 5 mL/min) and semi preparative analysis due to flow up to 10 mL/min. The 1260 Infinity II Multisampler can handle both vials and microtiter plates with ease and efficiency, and is designed for low carryover, as you can take clean to a whole new level with the multi-wash capability, cleaning all relevant injection parts between runs. This integrated feature flushes the injection needle outside with three solvents, and uses seat back flush procedures to reduce carryover to less than 9 ppm.

The Agilent 1260 Infinity II Variable Wavelength Detector (VWD) is the most sensitive and fastest detector in its class. The detector features time-programmable wavelength switching provides sensitivity and selectivity for your applications. More sample information can be acquired in the dual wavelength mode. Low detector noise (< ± 2.5 AU) and baseline drift (< 1 x 10^-4 AU/h) facilitates precise quantification of trace levels components. High productivity can be achieved with fast analysis at up to 120 Hz data rates. The Agilent 1260 Infinity II Multicolumn Thermostat (MCT) allows precise column thermostatting over a broad temperature range: from cooling down to 10 degrees below ambient temperature up to 85°C, providing high flexibility for optimized speed and selectivity of LC separation. For bio-inert applications bio-inert heat exchangers and a selection of bio-inert valves are offered by Agilent.

The Agilent 1260 Infinity II LC System w/ VWD can be fully controlled from the Agilent OpenLab CDS Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.

This Agilent system is in excellent condition and will be fully tested to perform at factory specifications before being shipped.

The Agilent 1260 Infinity II LC System w/ VWD includes:
  • > G7111B Quaternary Pump (With Integrated Degasser)
  • > G7114A VWD Detector
  • > G7167A Multisampler
  • > G7116A Multicolumn Thermostat
  • > Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
  • > OpenLab CDS Software Installed
  • > Can/Crossover Cables
  • > Agilent Lan Interface Card Built-In
  • > Solvent Tubing
  • > 4 x Bottles, Caps and Solvent Filters
  • > New Stainless Steel Capillaries
  • > 1/4" Wrench & Mobile Phase Priming Syringe
  • > Waste Tubing
  • > All Power Cords
  • > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
  • > Full 180 Day Parts and Labor Warranty

Below is a List of Diagnostic Tests Performed On This System
G7114A Variable Wavelength Detector Diagnostic Tests:
  • Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
  • Dark Current Test: The test measures the dark current from the detector optic.
  • Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
  • Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
  • Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
  • Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
G7111B Quaternary Pump Diagnostic Tests:
  • Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
  • Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
G7116A Multicolumn Thermostat Diagnostic Tests:
  • Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
G7167A Multisampler Diagnostic Tests:
  • Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
System Tests:
  • 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the Agilent 1260 Infinity II LC System w/ VWD.

Agilent 1260 Infinity II LC System w/ VWD Specifications

VWD Detector Specification

Detection Type

Double-beam photometer
Light Source Deuterium lamp
Wavelength Range 190 - 600 nm

< ± 0.25 x 10^-5 AU, at 230 nm (single wavelength detection)

< ± 0.80 x 10^-5 AU, at 230 nm and 254 nm (dual wavelength detection)

Drift < 1 x 10^-4 AU/h at 230 nm
Linearity > 2.5 AU upper limit
Wavelength Accuracy ± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
Wavelength Precision < ± 0.1 nm
Slit Width 6.5nm typical over whole wavelength range
Time Programmable Wavelength, polarity, peak width, lamp on/off
Flow Cell Standard: 14 µl volume, 10 mm cell path length and 40 bar (588 psi) pressure maximum
Spectral Tools Stop-flow wavelength scan

Click Here to View More Specifications for the Agilent 1260 Infinity II LC System w/ VWD

Quaternary Pump Specification
Hydraulic System Dual piston in series pump with servo-controlled variable stroke driven, powe transmission by gears and ball screws, floating pistons
Settable Flow Range Set points 0.001 - 10 ml/min, in 0.001 ml/min increments
Recommended Flow Range

0.02 - 10.0 ml/min

Flow Precision ≤ 0.07% RSD or ≤ 0.02 min SD
Flow Accuracy ± 1% or 10 µl/min, whichever is greater, pumping degassed H2O at 10 MPa (100 bar)
Pressure Operating Range

Up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min

Up to 20 MPa (200 bar, 2950 psi) up to 10 ml/min

Pressure Pulsation < 2% amplitude (typically < 1.0%) or < 0.3 MPa (3 bar, 44 psi), whichever is greater
Compressibility Compensation User-selectable, based on mobile phase compressibility
Recommended pH Range 1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient Formation Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay Volume 600 - 900 µl, dependent on back pressure; measured with water at 1 ml/min (water/caffeine tracer)
Settable Composition Range 0 - 100% in 0.1% increments
Composition Precision < 0.2% RSD or < 0.04 min SD, whatever is greater
Integrated Degassing Unit

Number of channels: 4

Internal volume per channel: 1.5 ml

Multisampler Specification
Injection Range 0.1 - 90 µl in 0.1 µl increments
Injection Precision < 0.15% RSD or SD < 10nL, whatever is greater
Injection Linearity 0.9999 in the range of 0.1 - 100 µl
Pressure Range Up to 800 bar
Sample Viscosity Range 0.2 - 5 cp
Sample Capacity 1H Drawer: up to 8 drawers and 16 positions
Shallow well plates (MTP)

2H Drawer: up to 4 drawers and 8 positions
MTP, deep well plates, vials, Eppendorf

3H Drawer: up to 2 drawers and 4 positions
MTP, deep well plates, vials up to 6 ml, Eppendorf
Injection Cycle Time < 10 seconds using following conditions:

Default draw speed: 100 ul/min
Default eject speed: 400 ul/min
Injection volume: 1 ul
Multiwash Outer needle wash and seat backflush for carryover reduction with up to 3 different solvents
Carry Over < 0.003% (30 ppm) Multisampler Standard Needle

<0.0009% (3 ppm) Multisampler Multiwash
Multicolumn Thermostat Specification
Temperature Range Ambient -10°C to 85°C (minimum 4°C)
Temperature Stability ± 0.1°C
Temperature Accuracy ± 0.5°C (with calibration for 40°C)
Temperature Precision 0.05°C
Independent Temperature Zones 2 in single device
Column Capacity 4 columns of up to 300 mm length plus InfinityLab Quick-Connect fittings or pre-column The number of precolumn Quick-Connect Heat Exchangers is scalable - each column can be equipped with individual heat exchanger for best performance 4-column selector valve is available to access each column without replumbing
Heat-Up / Cool-Down Time 5 min from ambient to 40°C
10 min from 40°C to 20°C
< 25 min from 25°C to 85°C

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All Lab Equipment is Fully Recertified and Tested

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