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Agilent 1260 Infinity I HPLC System w/ VWD & FLD
Agilent 1260 Infinity I HPLC System w/ VWD & FLD

Our Price: $47,595.00
Sale Price: $39,995.00
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Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report

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Quantity in Stock:1

Product Code: AG-1260HPLCFLD

Recertification Protocol
Technical Specification Sheet

Agilent 1260 Infinity I HPLC System w/ VWD & FLD

The Agilent 1260 Infinity I HPLC System w/ VWD & FLD offers the most flexibility for solvent selection and automation in HPLC method development, research and all HPLC applications requiring continuous access to a wide range of solvent choices. This system is ideally suited for multi-method, high-throughput workflows and offers convenient access to two solvents for isocratic or gradient analysis, for rapid method development and speeding up preparation of mobile phases and flushing the HPLC system.

The Agilent 1260 Infinity HPLC System's binary pump is comprises two identical pumps integrated into one housing. Binary gradients are created by high-pressure mixing. Typical applications are high throughput methods with fast gradients on high resolution 2.1 mm columns. The pump is capable of delivering flow in the range of 0.1 - 5 mL/min against up to 600 bar. Degassing of the solvents is done with the High Performance Degasser, which comprises four separate vacuum chambers with semipermeable tubings, a vacuum pump, and control assembly. The 1260 Infinity Autosampler is designed to offer the well-established flow-through design with variable volume injection and to achieve extremely low carryover, providing increased analysis speed with sensitivity, resolution, and precision. Paired with the autosampler is a thermostatted column compartment and ALS thermostat to serve as a temperature management system providing heating and cooling in order to meet extreme requirements of retention time reproducibility. The featured Variable Wavelength Detector (VWD) is designed for the highest optical UV performance, GLP compliance, and easy maintenance. The VWD features a deuterium lamp for the highest intensity and lowest detection limit over a wavelength range of 190 to 600 nm. This detector also features a dual-wavelength mode to acquire even more sample information, along with time-programmable wavelength switching to provide optimum sensitivity and selectivity for your applications. The featured Fluorescence Detector (FLD) brings high-sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. Some of the features of the FLD Detector include; a flash lamp for highest intensity and lowest detection limit, multi-wavelength mode for on-line spectra, spectra acquisition and simultaneous multi-signal detection, easy front access to the flow cell for fast replacement, and built-in wavelength accuracy verification. Users may also use an optional cuvette for off-line measurements.

The Agilent 1260 Infinity I HPLC System w/ VWD & FLD can be fully controlled from the included Agilent ChemStation Workstation Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.

This Agilent system is in excellent condition and will be fully tested to perform at factory specifications before being shipped.

The Agilent 1260 Infinity I HPLC System w/ VWD & FLD includes:
  • > G1312B Binary Pump
  • > G1329B Autosampler
  • > G1330B ALS Thermostat
  • > G1316C TCC
  • > G4225A HiP Degasser
  • > G1314C VWD Detector
  • > G1321B FLD Detector
  • > Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
  • > ChemStation Version 4.3 Software Installed
  • > Can/Crossover Cables
  • > Agilent Lan Interface Card Built-In
  • > Solvent Tubing
  • > 4 x Bottles, Caps and Solvent Filters
  • > New Stainless Steel Capillaries
  • > 1/4" Wrench & Mobile Phase Priming Syringe
  • > Waste Tubing
  • > All Power Cords
  • > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
  • > Full 180 Day Parts and Labor Warranty

Below is a List of Diagnostic Tests Performed On This System
G1314C Variable Wavelength Detector & G1321B Fluorescence Detector Diagnostic Tests:
  • Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
  • Dark Current Test: The test measures the dark current from the detector optic.
  • Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
  • Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
  • Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
  • Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
G1312B Binary Pump Diagnostic Tests:
  • Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
  • Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
G1316C Column Compartment Diagnostic Tests:
  • Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
G1329B Autosampler Diagnostic Tests:
  • Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
System Tests:
  • 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1260 Infinity HPLC System.

Agilent 1260 Infinity I HPLC System w/ VWD & FLD Specifications

VWD Detector Specification
Detection Type Double-beam photometer
Light Source Deuterium lamp
Wavelength Range 190 - 600 nm
Short Term Noise (ASTM) Single and Multi-Wavelength < ± 0.5 × 10^-5 AU at 254 nm
Drift 3 × 10^-4 AU/hr at 254 nm
Linearity > 2.0 AU (5%) upper limit
Band Width 6.5 nm typical
Maximum Data Rate 55 Hz
Signal Data Rate Up to 80 Hz
Spectra Data Rate Up to 80 Hz
Flow Cell

Standard: 14 µL volume, 10 mm cell path length and 40 bar (580 psi) pressure maximum

Control and Data Evaluation

Agilent ChemStation for LC

FLD Detector


Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h

Pulse frequency

296 Hz for single signal mode 74 Hz for economy mode

Excitation Monochromator

Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Reference System:

in-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Spectrum acquisition:

Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All

Wavelength characteristic

Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

Click Here to View More Specifications for the Agilent 1260 Infinity HPLC System

Binary Pump Specification
Hydraulic System Two dual piston in series pumps with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
Setable Flow Range 0.001 - 5 mL/min, in 0.001 mL/min increments
Flow Range

0.05 - 5.0 mL/min

Flow Precision < 0.07% RSD or < 0.02 min SD, whatever is greater
Flow Accuracy ± 1% or 10 µl/min, what ever is greater
Pressure Operating Range 0 - 60 MPa (0 - 600 bar, 0 - 8,700 psi) up to 5 mL/min
Pressure Pulsation < 2% amplitude or < 3 bar at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Compressibility Compensation Pre-defined, based on mobile phase compressibility
Recommended pH Range 1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient Formation High-pressure binary mixing
Delay Volume Standard delay volume configuration: 600 - 800 µl, (includes 400 µl mixer), dependent on back pressure

Low delay volume configuration: 120 µl
Autosampler Specification
Injection Range 0.1 - 100 µl in 0.1 µl increments
Precision < 0.25% from 5 - 40µl
< 0.5% from 2 - 5µl
< 0.7% from 1 - 2µl
< 1.5% from 0.5 - 1µl
Pressure Range Up to 600 bar (8,700 psi)
Sample Viscosity Range 0.2 - 5 cp
Sample Capacity 2 x well plates (MTP) + 10 x 2 ml vials
108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 ml vials
30 x 6 ml vials in 2 x 15 vial plate
100 Micro vial tray plus 10 additional 2 ml vials
54 Eppendorf tubes (0.5/1.5/2 ml) in 2 x 27 Eppendorf tube plates
Injection Cycle Time Typically < 21 seconds using default conditions and injection volume of 5µl
Carryover Typically < 0.004%
ALS Thermostat Specification
Temperature Range 4°C to 40°C in 1°C increments
Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50% 3°C to 8°C at a setpoint of 4°C
Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60% 3°C to 9°C at a setpoint of 4°C
Column Compartment Specification
Temperature Range Ambient -10°C to 80°C at flow rates up to 5 mL/min
Temperature Stability ± 0.15°C
Temperature Accuracy ± 0.8°C
Warm-Up/Cool-Down Time 5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
High Performance Degasser Specification
Number of Solvent Channels 4
Flow Range 0 - 10 mL/min per channel
Internal Volume Per Channel 0.45 mL per channel
Materials in Contact with Solvent TFE/PDD Copolymer, FEP, PEEK
pH Range 1 - 14

Computer Specifications


Dell Optiplex 7010 Desktop PC


Intel Core i5-3470 Processor 3.2GHz



Hard Drive

250GB Internal SSD

Operating System

Windows 10 Professional, 64bit


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