Agilent 1200 HPLC System with Variable Wavelength Detector and Fluorescence Detector

Standard: 13 µl volume, 10 mm cell

path length and 120 bar (1760 psi)

pressure maximum

output range 0.001 – 2 AU, two

outputs

Controller-area network (CAN), GPIB,

RS-232C, APG Remote: ready, start,

stop and shut-down signals, LAN

and display (through control module

and ChemStation), leak detection, safe

leak handling, leak output signal for

shutdown of pumping system. Low

voltages in major maintenance areas.

continuous tracking of instrument

usage in terms of lamp burn time with

user-settable limits and feedback

messages. Electronic records of

maintenance and errors. Verification of

wavelength accuracy with built-in

holmium oxide filter.

Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W

Pulse frequency

296 Hz for single signal mode 74 Hz for spectral mode

Excitation Monochromator

Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm

Reference System:

In-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Spectrum acquisition:

Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All

Wavelength characteristic

Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

Control and data evaluation

Agilent ChemStation for LC, Agilent Instant Pilot G4208A or Agilent Control Module G1323B with limited spectral data analysis and printing of spectra

Communications

Controller-area network (CAN), GPIB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals

Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W

Pulse frequency

296 Hz for single signal mode 74 Hz for spectral mode

Computer Specifications

Model

Dell Optiplex 7010 Desktop PC

Processor

Intel Core I5-6500 Processor 3.2GHz

RAM

16GB

Hard Drive

250GB Internal SSD

Operating System

Windows 10 Professional, 64bit