Agilent 1200 HPLC G1321A Fluorescence Detector

Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W

Pulse frequency

296 Hz for single signal mode 74 Hz for spectral mode

Excitation Monochromator

Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm

Reference System:

in-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Spectrum acquisition:

Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All

Wavelength characteristic

Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

Control and data evaluation

Agilent ChemStation for LC, Agilent Instant Pilot G4208A or Agilent Control Module G1323B with limited spectral data analysis and printing of spectra

Communications

Controller-area network (CAN), GPIB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals