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BD FACSCAlibur 2 Laser, 4 Color Flow Cytometer
BD FACSCAlibur 2 Laser, 4 Color Flow Cytometer
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Our Price: $21,995.00

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Condition: Refurbished and Tested to Specifications (Click for Info)
Warranty: 90-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 5 to 10 Business Days

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Quantity in Stock:1

Product Code: BF-FACS

Description Operator's Guide Marshall Scientific FAQ's

BD FACSCalibur 2 Laser, 4 Color Flow Cytometer

The Becton Dickinson FACSCalibur Flow Cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. The BD FACSCalibur is a fully integrated multiparameter system that has the performance and sensitivity to ensure objective and reproducible results through the use of dual-laser technology, interbeam compensation, and an alignment-free optical design. Efficient collection optics, coupled to the flow cell with an optical gel, permit the use of a low-powered, air-cooled argon laser and a red diode laser to ensure the high sensitivity required for analysis of low-density antigens.

The BD High Throughput Sampler (HTS) option provides flexible, easy-to-use automation. The HTS option allows users to rapidly acquire samples with the BD FACSCalibur flow cytometer in a microtiter plate. The HTS option supports 96 U-, V-, and flat-bottom plates as well as 384-well microtiter plates.

The BD FACSCaliber system is best used for routine cell analysis, assay development, verification, and identification of cellular populations of interest. Streamlined software analysis applications include enumeration of lymphocyte subsets, stem cells, residual white blood cells, reticulocytes, DNA analysis, immune function studies, bead based immunoassays, and multiplexed analysis of signal transduction and phosphorylation targets.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Includes: > Mac Computer Running CellQuest Pro Software > Low-Hours FacsFlo System > Optional High Throughput Sampler > Full 90 Day Parts and Labor Warranty

BD FACSCalibur 2 Laser, 4 Color Flow Cytometer Specifications

Fluorescence Sensitivity

Estimated detection limit is 750 molecules of equivalent soluble fluorescein

Fluorescence Resolution

Coefficient of variation in FL2-Area of <3%, full peak for propidium iodide-stained chicken erythrocyte nuclei

Forward and Side Scatter Sensitivity

Sensitivity enables the separation of fixed platelets from noise

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes

Optical Platform

Fixed optical assembly


15 milliwatt 488 nm, air-cooled Argon-ion laser; life expectancy >5,000 hours Optional second laser: nominally 635 nm

Beam Geometry

Prismatic expander and achromatic spherical lens provide 22 µm x 66 µm elliptical beam for argon ion laser, and nominally 15 µm x 61 µm elliptical beam for red diode laser

Optical Coupling

Quartz cuvette is coupled to emission lens by refractive index matching optical gel for optimum collection efficiency

Background Rejection

Obscuration blade and slit minimize unwanted laser radiation at the detector

Forward Scatter Detector and Filter

High performance solid state silicon detector with 488 nm band pass filter for clear signal detection, and red diode laser signal rejection

Side Scatter Detector

High performance photomultiplier using Brewster angle beam splitter in the emission optical train

Fluorescence Detectors and Filters

Four high performance, high dynamic range photomultipliers with band pass filters: 530 nm (FITC), 585 nm (PE/PI), 661 nm (APC), and >650 nm (PerCP) with base unit, >670 nm (PerCP) with FL4 option

BD FACSCalibur 2 Cytometer Manual

Click Here to View all the Technical Specs for the FACSCalibur

Why Buy From Marshall Scientific?

With over 3,500 university, research, and hospital labs served, Marshall Scientific boasts a 99.8% customer satisfaction rate with less than 1% instrument failure in the field.

Our A+ Better Business Bureau rating confirms our business integrity. Marshall Scientific promises to be transparent, honest, and responsive throughout the purchase process.

By outfitting your lab with Marshall Scientific, you can save up to 70% with our refurbished instrument inventory - all guaranteed to work and under our full parts and labor warranty.

Marshall Scientific is not an equipment broker or an auction company. All of our products are evaluated and refurbished by our trained technicians.

Unlike other used or refurbished sellers, Marshall Scientific offers completely transparent product stock and pricing. You can easily see what we have and how much it costs.

All of Marshall Scientific’s products are carefully packaged, crated, and insured to protect your equipment and will be ready for immediate use in your lab.

Rest assured, our devoted technical and customer service team is with you before, during, and after your purchase to insure everything works the way it should.

Full 90-Day Parts & Labor Protection

Marshall Scientific's Refurbishment Protocol


Our technicians decontaminate and clean all equipment upon arrival and upon purchase to create a neutral environment for lab use.

Preliminary testing is conducted to identify potential product defects. Upon completion, we replace all faulty and/or under-performing parts with original manufacturer parts that perform up to specification.

Any parts known to fail or malfunction from excessive use are removed and replaced to insure the extended lifetime of your equipment.

Any necessary preventative maintenance is performed alongside repairs, so your purchase will be ready to go as soon as it arrives.

All electronics and boards are tested for proper function and response by our industry trained technicians. Any electronic deficiencies detected are immediately repaired.

Final testing cycles ensure your instrument performs fully at manufacturer specifications, guaranteeing you receive a properly working product ready for use.

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