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BD FACSCAlibur 2 Laser, 4 Color Flow Cytometer
BD FACSCAlibur 2 Laser, 4 Color Flow Cytometer
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Our Price: $18,995.00

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Condition: Refurbished and Tested to Manufacturer Spec
Warranty: 90 Day Full Parts and Labor (Click for Info)
Typically Ships Within: 7 to 10 Business Days

Quantity in Stock:1
Product Code: BF-FACS

Description Operator's Guide Marshall Scientific FAQ's

BD FACSCalibur 2 Laser, 4 Color Flow Cytometer

The Becton Dickinson FACSCalibur Flow Cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. The BD FACSCalibur is a fully integrated multiparameter system that has the performance and sensitivity to ensure objective and reproducible results through the use of dual-laser technology, interbeam compensation, and an alignment-free optical design. Efficient collection optics, coupled to the flow cell with an optical gel, permit the use of a low-powered, air-cooled argon laser and a red diode laser to ensure the high sensitivity required for analysis of low-density antigens.

The BD High Throughput Sampler (HTS) option provides flexible, easy-to-use automation. The HTS option allows users to rapidly acquire samples with the BD FACSCalibur flow cytometer in a microtiter plate. The HTS option supports 96 U-, V-, and flat-bottom plates as well as 384-well microtiter plates.

The BD FACSCaliber system is best used for routine cell analysis, assay development, verification, and identification of cellular populations of interest. Streamlined software analysis applications include enumeration of lymphocyte subsets, stem cells, residual white blood cells, reticulocytes, DNA analysis, immune function studies, bead based immunoassays, and multiplexed analysis of signal transduction and phosphorylation targets.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Includes: > Mac Computer Running CellQuest Pro Software > Low-Hours FacsFlo System > Optional High Throughput Sampler > Full 90 Day Parts and Labor Warranty

BD FACSCalibur 2 Laser, 4 Color Flow Cytometer Specifications

Fluorescence Sensitivity

Estimated detection limit is 750 molecules of equivalent soluble fluorescein

Fluorescence Resolution

Coefficient of variation in FL2-Area of <3%, full peak for propidium iodide-stained chicken erythrocyte nuclei

Forward and Side Scatter Sensitivity

Sensitivity enables the separation of fixed platelets from noise

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes

Optical Platform

Fixed optical assembly


15 milliwatt 488 nm, air-cooled Argon-ion laser; life expectancy >5,000 hours Optional second laser: nominally 635 nm

Beam Geometry

Prismatic expander and achromatic spherical lens provide 22 µm x 66 µm elliptical beam for argon ion laser, and nominally 15 µm x 61 µm elliptical beam for red diode laser

Optical Coupling

Quartz cuvette is coupled to emission lens by refractive index matching optical gel for optimum collection efficiency

Background Rejection

Obscuration blade and slit minimize unwanted laser radiation at the detector

Forward Scatter Detector and Filter

High performance solid state silicon detector with 488 nm band pass filter for clear signal detection, and red diode laser signal rejection

Side Scatter Detector

High performance photomultiplier using Brewster angle beam splitter in the emission optical train

Fluorescence Detectors and Filters

Four high performance, high dynamic range photomultipliers with band pass filters: 530 nm (FITC), 585 nm (PE/PI), 661 nm (APC), and >650 nm (PerCP) with base unit, >670 nm (PerCP) with FL4 option

BD FACSCalibur 2 Cytometer Manual

Click Here to View all the Technical Specs for the FACSCalibur
Warranty Information

  • This item, purchased from Marshall Scientific, is covered by a 90-day full parts and labor warranty. This warranty covers any defects in material or workmanship within the 90-day period. Marshall Scientific will repair or replace the defective items with OEM parts at no charge. To receive warranty service, please contact us to determine the problem and best solution possible for you. To view Marshall Scientific's warranty certificate Click Here.

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