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Amersham Biosciences AKTA FPLC System
Amersham Biosciences AKTA FPLC System
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Amersham Biosciences AKTA FPLC System


The Amersham Biosciences AKTA FPLC System is a biocompatible, high performance liquid chromatography system for the fast and easy purification of proteins. Providing flexibility and reliability in every run, the AKTA system provides a unique combination of flow configuration and methods support for all chromatographic techniques including gel filtration (size exclusion) chromatography, anion exchange chromatography, cation exchange chromatography, hydrophobic interaction chromatography, and affinity chromatography. The compact and flexible design with an expandable rack allows the use of longer chromatographic columns and automation kits. The AKTA FPLC fully meets the demands of modern purification media by utilizing AKTAdesign and UNICORN to provide reliable results and system versatility. This system features flow rates up to 20 ml/min and pressures up to 5 MPa across one working platform for scale-up production.

The P-920 pump provides highly accurate and reproducible flow rates over the whole pressure range. Its chemical resistance makes it possible to use with corrosive liquids, such as organic solvents, as well as with high salt aqueous solutions. The P-920's high flow range makes it suitable for both analytical and preparative chromatography, and it is designed to work with a wide range of columns and gels from the UPC-900 monitor. The monitor provides precise on-line measurements of UV absorption, pH, and conductivity in liquid chromatography. The UPC-900 offers fixed wavelengths of 214 nm, 254 nm, or 280 nm with fast response. Also included is the M-925 mixer for a dynamic, single chamber unit which provides accuracy and reproducibility. The seven-port motorized valve, INV-907, can easily be used to load or wash the sample loop or wash the pump. The Frac-920 provides fraction collection up to 175 fractions in 12 mm tubes, or 95 fractions using 10-18mm tubes, or 40 fractions with 30 mm diameter tubes, depending on which rack is being used.


All of our equipment is fully tested to perform at factory specifications before being shipped.


Amersham Biosciences AKTA FPLC System Includes: > IBM Windows 7 workstation loaded with Unicorn 5.31 Software > UPC-900 module (U=UV; P=pH; C=Conductivity) > P-920 module (with syringe pump) > CU 950 Communications Module > M-925 Mixer (Outlet Valves) > INV-907 (Injection Valve) > Conductivity Flow Cell > PH Probe > Optical Module > Frac-920 (Fraction Collector) > Gradient Linearity and Step Response Tests > All Cables and Power Cords > Full 90 Day Parts and Labor Warranty


Amersham Biosciences AKTA FPLC Specifications

Pump P-920

Flow rate range isocratic mode gradient mode

0.05–20 ml/min in steps of 10 µl/min 0.1–20 ml/min in steps of 10 µl/min

Pressure range

0–5 MPa (50 bar, 725 psi)

Pressure pulsation

Max. 6% (dP/P) during pump stroke

pH stability range

1–13 (1–14 < 1 day exposure)

Viscosity

< 10 ml/min

> 10 ml/min

Max. 10cP

Max. 5 cP

Flow rate reproducibility

flow rate 0.5–10 ml/min

flow rate 10–20 ml/min

rsd < 0.2%

rsd < 0.5%

Gradient composition

accuracy between turnings

accuracy during turnings

reproducibility

± 2% at 0.5–5 ml/min and < 5 MPa

± 2% at 0.5–5 ml/min and 0.5–2.0 MPa

rsd < 0.5% at 0.5–20 ml/min and < 5 MPa

Leakage

< 0.5 µl/min (pump module A and B each)

Monitor UPC-900 UV measurement

Absorbance range

0.01–5.0 AU (full scale)

Autozero range

-0.2–2.0 AU

Baseline adjust

Adjustable 0–100% of full scale

Wavelengths

Hg lamp, fixed

by changing filter

Zn lamp

254 and 280 nm

313, 365, 405, 436 and 546 nm

214 nm

UV Flow cell, 2 mm

Flow rate

0–100 ml/min

Max. pressure

4.0 MPa (40 bar, 580 psi)

Max. back-pressure

0.05 MPa at 100 ml/min

Liquid temperature range

+4 to +60 °C

Optical path length

2 mm

Cell volume

2 µl (30 µl detector volume)

UV flow cell, 5 mm

Flow rate

0–20 ml/min

Max. pressure

4.0 MPa (40 bar, 580 psi)

Max. back-pressure

0.02 MPa at 20 ml/min

Optical path length

5 mm

Cell volume

6 µl (10 µl detector volume)

Conductivity measurement

Flow rate

0–100 ml/min

Max. pressure

5 MPa (50 bar, 725 psi)

Max. back-pressure

0.01 MPa at 100 ml/min

pH measurement

pH range

0 to 14



Amersham Biosciences AKTA Manual

Click Here to View all the Technical Specs for the AKTA FPLC System

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Full 90-Day Parts & Labor Protection

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