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Amersham Biosciences AKTA FPLC System
Amersham Biosciences AKTA FPLC System
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Condition: Refurbished and Tested to Manufacturer Spec
Warranty: 90 Day Full Parts and Labor (Click for Info)
Typically Ships Within: 5 to 7 Business Days

Quantity in Stock:2
Product Code: AK-FPB

Description Marshall Scientific FAQ's

Amersham Biosciences AKTA FPLC System

The Amersham Biosciences AKTA FPLC System is a biocompatible, high performance liquid chromatography system for the fast and easy purification of proteins. Providing flexibility and reliability in every run, the AKTA system provides a unique combination of flow configuration and methods support for all chromatographic techniques including gel filtration (size exclusion) chromatography, anion exchange chromatography, cation exchange chromatography, hydrophobic interaction chromatography, and affinity chromatography. The compact and flexible design with an expandable rack allows the use of longer chromatographic columns and automation kits. The AKTA FPLC fully meets the demands of modern purification media by utilizing AKTAdesign and UNICORN to provide reliable results and system versatility.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Amersham Biosciences AKTA FPLC System Includes: > IBM Windows 7 workstation loaded with Unicorn 5.31 Software > UPC-900 module (U=UV; P=pH; C=Conductivity) > P-920 module (with syringe pump) > CU 950 Communications Module > M-925 Mixer (Outlet Valves) > INV-907 (Injection Valve) > Conductivity Flow Cell > PH Probe > Optical Module > Frac-920 (Fraction Collector) > Gradient Linearity and Step Response Tests > All Cables and Power Cords > Full 90 Day Parts and Labor Warranty

Amersham Biosciences AKTA FPLC Specifications

Pump P-920

Flow rate range isocratic mode gradient mode

0.05–20 ml/min in steps of 10 µl/min 0.1–20 ml/min in steps of 10 µl/min

Pressure range

0–5 MPa (50 bar, 725 psi)

Pressure pulsation

Max. 6% (dP/P) during pump stroke

pH stability range

1–13 (1–14 < 1 day exposure)


< 10 ml/min

> 10 ml/min

Max. 10cP

Max. 5 cP

Flow rate reproducibility

flow rate 0.5–10 ml/min

flow rate 10–20 ml/min

rsd < 0.2%

rsd < 0.5%

Gradient composition

accuracy between turnings

accuracy during turnings


± 2% at 0.5–5 ml/min and < 5 MPa

± 2% at 0.5–5 ml/min and 0.5–2.0 MPa

rsd < 0.5% at 0.5–20 ml/min and < 5 MPa


< 0.5 µl/min (pump module A and B each)

Monitor UPC-900 UV measurement

Absorbance range

0.01–5.0 AU (full scale)

Autozero range

-0.2–2.0 AU

Baseline adjust

Adjustable 0–100% of full scale


Hg lamp, fixed

by changing filter

Zn lamp

254 and 280 nm

313, 365, 405, 436 and 546 nm

214 nm

UV Flow cell, 2 mm

Flow rate

0–100 ml/min

Max. pressure

4.0 MPa (40 bar, 580 psi)

Max. back-pressure

0.05 MPa at 100 ml/min

Liquid temperature range

+4 to +60 °C

Optical path length

2 mm

Cell volume

2 µl (30 µl detector volume)

UV flow cell, 5 mm

Flow rate

0–20 ml/min

Max. pressure

4.0 MPa (40 bar, 580 psi)

Max. back-pressure

0.02 MPa at 20 ml/min

Optical path length

5 mm

Cell volume

6 µl (10 µl detector volume)

Conductivity measurement

Flow rate

0–100 ml/min

Max. pressure

5 MPa (50 bar, 725 psi)

Max. back-pressure

0.01 MPa at 100 ml/min

pH measurement

pH range

0 to 14

Click Here to View all the Technical Specs for the AKTA FPLC System
Warranty Information

  • This item, purchased from Marshall Scientific, is covered by a 90-day full parts and labor warranty. This warranty covers any defects in material or workmanship within the 90-day period. Marshall Scientific will repair or replace the defective items with OEM parts at no charge. To receive warranty service, please contact us to determine the problem and best solution possible for you. To view Marshall Scientific's warranty certificate Click Here.

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