BD FACSCalibur 2 Laser, 4 Color Flow Cytometer
The Becton Dickinson FACSCalibur Flow Cytometer offers a
unique approach to flow cytometry, allowing users to perform both cell analysis
and cell sorting in a single system, providing flexibility for a variety of
research and clinical applications. The BD FACSCalibur is a fully integrated
multiparameter system that has the performance and sensitivity to ensure objective
and reproducible results through the use of dual-laser technology, interbeam
compensation, and an alignment-free optical design. Efficient collection
optics, coupled to the flow cell with an optical gel, permit the use of a
low-powered, air-cooled argon laser and a red diode laser to ensure the high
sensitivity required for analysis of low-density antigens.
The BD High Throughput Sampler (HTS) option provides flexible,
easy-to-use automation. The HTS option allows users to rapidly acquire samples
with the BD FACSCalibur flow cytometer in a microtiter plate. The HTS option
supports 96 U-, V-, and flat-bottom plates as well as 384-well microtiter
plates.
The BD FACSCaliber system is best used for routine cell
analysis, assay development, verification, and identification of cellular
populations of interest. Streamlined software analysis applications include enumeration
of lymphocyte subsets, stem cells, residual white blood cells, reticulocytes,
DNA analysis, immune function studies, bead based immunoassays, and multiplexed
analysis of signal transduction and phosphorylation targets.
All of our equipment is fully tested to perform at factory
specifications before being shipped.
Becton Dickinson Facscalibur Flow Cytometer Includes:
Includes:
- Mac G5 Computer w/ CellQuest Pro Software
- Software Dongle
- 2 Lasers, 4 Colors
- Flatscreen Monitor
- Calibration Report
- All Power Cords, Tubing, Hoses
- Full 180 Day Parts and Labor Warranty
BD FACSCalibur 2 Laser, 4 Color Flow Cytometer Specifications
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Fluorescence Sensitivity
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Estimated detection limit is 750 molecules of equivalent soluble
fluorescein
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Fluorescence Resolution
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Coefficient of variation in FL2-Area of <3%, full peak for
propidium iodide-stained chicken erythrocyte nuclei
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Forward and Side Scatter Sensitivity
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Sensitivity enables the separation of fixed platelets from noise
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Forward and Side Scatter Resolution
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Scatter performance is optimized for resolving lymphocytes,
monocytes, and granulocytes
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Optical Platform
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Fixed optical assembly
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Lasers
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15 milliwatt 488 nm, air-cooled Argon-ion laser; life expectancy
>5,000 hours Optional second laser: nominally 635 nm
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Beam Geometry
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Prismatic expander and achromatic spherical lens provide 22 µm x 66
µm elliptical beam for argon ion laser, and nominally 15 µm x 61 µm
elliptical beam for red diode laser
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Optical Coupling
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Quartz cuvette is coupled to emission lens by
refractive index matching optical gel for optimum collection efficiency
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Background Rejection
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Obscuration blade and slit minimize unwanted laser radiation at the
detector
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Forward Scatter Detector and Filter
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High performance solid state silicon detector with 488 nm band pass
filter for clear signal detection, and red diode laser signal rejection
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Side Scatter Detector
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High performance photomultiplier using Brewster angle beam splitter
in the emission optical train
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Fluorescence Detectors and Filters
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Four high performance, high dynamic range photomultipliers with band
pass filters: 530 nm (FITC), 585 nm (PE/PI), 661 nm (APC), and >650 nm
(PerCP) with base unit, >670 nm (PerCP) with FL4 option
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BD FACSCalibur 2 Cytometer Manual
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